 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, September 15, 1999, 19(18):7757-7769
Differential Expression of Brain-Derived Neurotrophic Factor, Neurotrophin-3, and Neurotrophin-4/5 in the Adult Rat Spinal Cord: Regulation by the Glutamate Receptor Agonist Kainic Acid
Isobel A. Scarisbrick1, 2, Paul J. Isackson1, and Anthony J. Windebank2 1 Department of Biochemistry and Molecular Biology, Mayo Clinic Jacksonville, Jacksonville, Florida 32224, and 2 Molecular Neuroscience Research, Mayo Clinic Rochester, Rochester, Minnesota 55905
Previous in vitro studies indicate that select members of the neurotrophin gene family, namely brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4/5), contribute to survival and differentiation of spinal cord motoneurons. To investigate the potential roles of these factors in the adult spinal cord, we examined their cellular localization and regulation after systemic exposure to an excitotoxic stimulus, kainic acid (KA).
Of the neurotrophins examined, NT-4/5 mRNA was most robustly expressed in the lumbosacral spinal cord of the normal adult rat, including expression by neurons throughout the gray matter, and in a subpopulation of white and gray matter glia. Both BDNF and NT-3 mRNAs were also densely expressed by
motoneurons of lamina IX, but were detected at lower levels elsewhere in the gray matter. NT-3 mRNA was additionally expressed by spinal cord glia, but was less widespread compared to NT-4/5. In response to systemic administration of KA, NT-4/5 and BDNF mRNAs were dramatically upregulated in a spatially and temporally restricted fashion, whereas levels of NT-3 mRNA were unchanged. These results provide strong in vivo evidence to support the idea that BDNF, NT-3, and in particular, NT-4/5, play a role in the normal function of the adult spinal cord. Furthermore, our results indicate that the actions of BDNF and NT-4/5 participate in the response of the cord to excitotoxic stimuli, and that those of NT-4/5 and NT-3 include both neurons and glia.
Key words: neurotrophin; motoneuron; glia; oligodendrocyte; kainic acid; spinal cord injury
Copyright © 1999 Society for Neuroscience 0270-6474/99/19187757-13$05.00/0
This article has been cited by other articles:
I. A. Scarisbrick, S. I. Blaber, C. F. Lucchinetti, C. P. Genain, M. Blaber, and M. Rodriguez
Activity of a newly identified serine protease in CNS demyelination
Brain, June 1, 2002; 125(6): 1283 - 1296.
[Abstract] [Full Text] [PDF]
O. Raineteau, K. Fouad, P. Noth, M. Thallmair, and M. E. Schwab
Functional switch between motor tracts in the presence of the mAb IN-1 in the adult rat
PNAS, May 24, 2001; (2001) 111165498.
[Abstract] [Full Text] [PDF]
J. Widenfalk, K. Lundstromer, M. Jubran, S. Brene, and L. Olson
Neurotrophic Factors and Receptors in the Immature and Adult Spinal Cord after Mechanical Injury or Kainic Acid
J. Neurosci., May 15, 2001; 21(10): 3457 - 3475.
[Abstract] [Full Text] [PDF]
V. L. Arvanov, B. S. Seebach, and L. M. Mendell
NT-3 Evokes an LTP-Like Facilitation of AMPA/Kainate Receptor-Mediated Synaptic Transmission in the Neonatal Rat Spinal Cord
J Neurophysiol, August 1, 2000; 84(2): 752 - 758.
[Abstract] [Full Text] [PDF]
O. Raineteau, K. Fouad, P. Noth, M. Thallmair, and M. E. Schwab
Functional switch between motor tracts in the presence of the mAb IN-1 in the adult rat
PNAS, June 5, 2001; 98(12): 6929 - 6934.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|