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The Journal of Neuroscience, October 1, 1999, 19(19):8260-8270
An Olfactory Sensory Neuron Line, Odora, Properly Targets Olfactory Proteins and Responds to Odorants
Julie R. Murrell1 and Dale D. Hunter1, 2 1 Program in Cell,
Molecular, and Developmental Biology and Departments of Neuroscience, Anatomy and Cell Biology, and 2 Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts 02111 The site for interactions between the nervous system and much of the chemical world is in the olfactory sensory neuron (OSN). Odorant receptor proteins (ORPs) are postulated to mediate these interactions. However, the function of most ORPs has not been demonstrated in vivo or in vitro. For this and other reasons, we created a conditionally immortalized cell line derived from the OSN lineage, which we term odora. Odora cells, under control conditions, are phenotypically similar to the OSN progenitor, the globose basal cell. After differentiation, odora cells more closely resemble OSNs. Differentiated odora cells express neuronal and olfactory markers, including components of the olfactory signal transduction pathway. Unlike other cell lines, they also efficiently target exogenous ORPs to their surface. Strikingly, differentiated odora cells expressing ORPs respond to odorants, as measured by an influx of calcium. In particular, cells expressing one ORP demonstrate a specific response to only one type of tested odorant. Odora cells, therefore, are ideal models to examine the genesis and function of olfactory sensory neurons.
Key words: olfactory epithelium; globose basal cell; olfactory sensory neuron; olfactory receptor protein; odorant signal transduction; conditional immortalization
Copyright © 1999 Society for Neuroscience 0270-6474/99/19198260-11$05.00/0
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