QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (88) Citing Articles via Google Scholar Google Scholar Articles by Tsuboi, A. Articles by Sakano, H. Search for Related Content PubMed PubMed Citation Articles by Tsuboi, A. Articles by Sakano, H.

 Previous Article  |  Next Article 

The Journal of Neuroscience, October 1, 1999, 19(19):8409-8418

Olfactory Neurons Expressing Closely Linked and Homologous Odorant Receptor Genes Tend to Project Their Axons to Neighboring Glomeruli on the Olfactory Bulb

Akio Tsuboi¹, Sei-ichi Yoshihara¹, Nika Yamazaki², Hiroaki Kasai², Hisae Asai-Tsuboi², Madoka Komatsu¹, Shou Serizawa¹, Tomohiro Ishii¹, Yoichi Matsuda³, Fumikiyo Nagawa¹, and Hitoshi Sakano^{1, 2}

¹ Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo 113-0032, Japan, ² Department of Cell Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan, and ³ Laboratory of Animal Genetics, Graduate School of Agricultural Sciences, Nagoya University, Nagoya 464-8601, Japan

We have characterized two separate odorant receptor (OR) gene clusters to examine how olfactory neurons expressing closely linked and homologous OR genes project their axons to the olfactory bulb. Murine OR genes, MOR28, MOR10, and MOR83, share 75-95% similarities in the amino acid sequences and are tightly linked on chromosome 14. In situ hybridization has demonstrated that the three genes are expressed in the same zone, at the most dorsolateral and ventromedial portions of the olfactory epithelium, and are rarely expressed simultaneously in individual neurons. Furthermore, we have found that olfactory neurons expressing MOR28, MOR10, or MOR83 project their axons to very close but distinct subsets of glomeruli on the medial and lateral sides of the olfactory bulb. Similar results have been obtained with another murine OR gene cluster for A16 and MOR18 on chromosome 2, sharing 91% similarity in the amino acid sequences. These results may indicate an intriguing possibility that olfactory neurons expressing homologous OR genes within a cluster tend to converge their axons to proximal but distinct subsets of glomeruli. These lines of study will shed light on the molecular basis of topographical projection of olfactory neurons to the olfactory bulb.

Key words: axonal projection; odorant receptor gene; olfactory bulb; olfactory epithelium; olfactory sensory map; gene cluster

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19198409-10$05.00/0

This article has been cited by other articles:

				H. Nishizumi, K. Kumasaka, N. Inoue, A. Nakashima, and H. Sakano Deletion of the core-H region in mice abolishes the expression of three proximal odorant receptor genes in cis PNAS, December 11, 2007; 104(50): 20067 - 20072. [Abstract] [Full Text] [PDF]

				J. H. Cho, M. Lepine, W. Andrews, J. Parnavelas, and J.-F. Cloutier Requirement for Slit-1 and Robo-2 in Zonal Segregation of Olfactory Sensory Neuron Axons in the Main Olfactory Bulb J. Neurosci., August 22, 2007; 27(34): 9094 - 9104. [Abstract] [Full Text] [PDF]

				K. Mori, Y. K. Takahashi, K. M. Igarashi, and M. Yamaguchi Maps of Odorant Molecular Features in the Mammalian Olfactory Bulb Physiol Rev, April 1, 2006; 86(2): 409 - 433. [Abstract] [Full Text] [PDF]

				J. W. Scott Sniffing and Spatiotemporal Coding in Olfaction Chem Senses, February 1, 2006; 31(2): 119 - 130. [Abstract] [Full Text] [PDF]

				K. Miyamichi, S. Serizawa, H. M. Kimura, and H. Sakano Continuous and Overlapping Expression Domains of Odorant Receptor Genes in the Olfactory Epithelium Determine the Dorsal/Ventral Positioning of Glomeruli in the Olfactory Bulb J. Neurosci., April 6, 2005; 25(14): 3586 - 3592. [Abstract] [Full Text] [PDF]

				S.-i. Yoshihara, K. Omichi, M. Yanazawa, K. Kitamura, and Y. Yoshihara Arx homeobox gene is essential for development of mouse olfactory system Development, February 15, 2005; 132(4): 751 - 762. [Abstract] [Full Text] [PDF]

				K. M. Igarashi and K. Mori Spatial Representation of Hydrocarbon Odorants in the Ventrolateral Zones of the Rat Olfactory Bulb J Neurophysiol, February 1, 2005; 93(2): 1007 - 1019. [Abstract] [Full Text] [PDF]

				H. Lei, T. A. Christensen, and J. G. Hildebrand Spatial and Temporal Organization of Ensemble Representations for Different Odor Classes in the Moth Antennal Lobe J. Neurosci., December 8, 2004; 24(49): 11108 - 11119. [Abstract] [Full Text] [PDF]

				Y. K. Takahashi, M. Kurosaki, S. Hirono, and K. Mori Topographic Representation of Odorant Molecular Features in the Rat Olfactory Bulb J Neurophysiol, October 1, 2004; 92(4): 2413 - 2427. [Abstract] [Full Text] [PDF]

				H. Tian and M. Ma Molecular Organization of the Olfactory Septal Organ J. Neurosci., September 22, 2004; 24(38): 8383 - 8390. [Abstract] [Full Text] [PDF]

				A. A. Nikonov and J. Caprio Odorant Specificity of Single Olfactory Bulb Neurons to Amino Acids in the Channel Catfish J Neurophysiol, July 1, 2004; 92(1): 123 - 134. [Abstract] [Full Text] [PDF]

				D. A. Wilson, M. L. Fletcher, and R. M. Sullivan Acetylcholine and Olfactory Perceptual Learning Learn. Mem., January 1, 2004; 11(1): 28 - 34. [Abstract] [Full Text] [PDF]

				R. Hoppe, H. Frank, H. Breer, and J. Strotmann The Clustered Olfactory Receptor Gene Family 262: Genomic Organization, Promotor Elements, and Interacting Transcription Factors Genome Res., December 1, 2003; 13(12): 2674 - 2685. [Abstract] [Full Text] [PDF]

				M. L. Fletcher and D. A. Wilson Olfactory Bulb Mitral-Tufted Cell Plasticity: Odorant-Specific Tuning Reflects Previous Odorant Exposure J. Neurosci., July 30, 2003; 23(17): 6946 - 6955. [Abstract] [Full Text] [PDF]

				M. A. Carlsson and B. S. Hansson Dose-Response Characteristics of Glomerular Activity in the Moth Antennal Lobe Chem Senses, May 1, 2003; 28(4): 269 - 278. [Abstract] [Full Text] [PDF]

				M. Alenius and S. Bohm Differential function of RNCAM isoforms in precise target selection of olfactory sensory neurons Development, March 1, 2003; 130(5): 917 - 927. [Abstract] [Full Text] [PDF]

				H. Hamana, J. Hirono, M. Kizumi, and T. Sato Sensitivity-dependent Hierarchical Receptor Codes for Odors Chem Senses, February 1, 2003; 28(2): 87 - 104. [Abstract] [Full Text] [PDF]

				K. Kobayakawa, R. Hayashi, K. Morita, K. Miyamichi, Y. Oka, A. Tsuboi, and H. Sakano Stomatin-Related Olfactory Protein, SRO, Specifically Expressed in the Murine Olfactory Sensory Neurons J. Neurosci., July 15, 2002; 22(14): 5931 - 5937. [Abstract] [Full Text] [PDF]

				J. M. Young and B. J. Trask The sense of smell: genomics of vertebrate odorant receptors Hum. Mol. Genet., May 15, 2002; 11(10): 1153 - 1160. [Abstract] [Full Text] [PDF]

				B. Key and J. St John Axon Navigation in the Mammalian Primary Olfactory Pathway: Where to Next? Chem Senses, March 1, 2002; 27(3): 245 - 260. [Abstract] [Full Text] [PDF]

				R. P. Lane, J. C. Roach, I. Y. Lee, C. Boysen, A. Smit, B. J. Trask, and L. Hood Genomic Analysis of the Olfactory Receptor Region of the Mouse and Human T-Cell Receptor alpha /delta Loci Genome Res., January 1, 2002; 12(1): 81 - 87. [Abstract] [Full Text] [PDF]

				S. H. Fuss and S. I. Korsching Odorant Feature Detection: Activity Mapping of Structure Response Relationships in the Zebrafish Olfactory Bulb J. Neurosci., November 1, 2001; 21(21): 8396 - 8407. [Abstract] [Full Text] [PDF]

				M. Pyrski, Z. Xu, E. Walters, D. J. Gilbert, N. A. Jenkins, N. G. Copeland, and F. L. Margolis The OMP-lacZ Transgene Mimics the Unusual Expression Pattern of OR-Z6, a New Odorant Receptor Gene on Mouse Chromosome 6: Implication for Locus-Dependent Gene Expression J. Neurosci., July 1, 2001; 21(13): 4637 - 4648. [Abstract] [Full Text] [PDF]

				L. Belluscio and L. C. Katz Symmetry, Stereotypy, and Topography of Odorant Representations in Mouse Olfactory Bulbs J. Neurosci., March 15, 2001; 21(6): 2113 - 2122. [Abstract] [Full Text] [PDF]

				J. M. Christie, N. E. Schoppa, and G. L. Westbrook Tufted Cell Dendrodendritic Inhibition in the Olfactory Bulb Is Dependent on NMDA Receptor Activity J Neurophysiol, January 1, 2001; 85(1): 169 - 173. [Abstract] [Full Text] [PDF]

				A. Ehlers, S. Beck, S. A. Forbes, J. Trowsdale, A. Volz, R. Younger, and A. Ziegler MHC-Linked Olfactory Receptor Loci Exhibit Polymorphism and Contribute to Extended HLA/OR-Haplotypes Genome Res., December 1, 2000; 10(12): 1968 - 1978. [Abstract] [Full Text]

				J. Strotmann, S. Conzelmann, A. Beck, P. Feinstein, H. Breer, and P. Mombaerts Local Permutations in the Glomerular Array of the Mouse Olfactory Bulb J. Neurosci., September 15, 2000; 20(18): 6927 - 6938. [Abstract] [Full Text] [PDF]

				F. Xu, I. Kida, F. Hyder, and R. G. Shulman Assessment and discrimination of odor stimuli in rat olfactory bulb by dynamic functional MRI PNAS, September 5, 2000; (2000) 180321397. [Abstract] [Full Text]

				R. P. Lane, T. Cutforth, R. Axel, L. Hood, and B. J. Trask Sequence analysis of mouse vomeronasal receptor gene clusters reveals common promoter motifs and a history of recent expansion PNAS, January 8, 2002; 99(1): 291 - 296. [Abstract] [Full Text] [PDF]

				F. Xu, I. Kida, F. Hyder, and R. G. Shulman Assessment and discrimination of odor stimuli in rat olfactory bulb by dynamic functional MRI PNAS, September 12, 2000; 97(19): 10601 - 10606. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help