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The Journal of Neuroscience, January 15, 1999, 19(2):674-683
Modulation of Synaptic GABAA Receptor Function by PKA and PKC in Adult Hippocampal Neurons
Pierrick Poisbeau2, Michael C. Cheney1, Michael D. Browning3, and Istvan Mody1 1 Departments of Neurology and Physiology, University of California at Los Angeles, School of Medicine, Los Angeles, California 90095, 2 Laboratoire de Neurophysiologie Cellulaire et Intégrée, Centre National de la Recherche Scientifique UMR 7519, Université Louis Pasteur, 67084 Strasbourg, France, and 3 Department of Pharmacology, University of Colorado Health Science Center, Denver, Colorado 80262
Several protein kinases are known to phosphorylate Ser/Thr residues of certain GABAA receptor subunits. Yet, the effect of phosphorylation on GABAA receptor function in neurons remains controversial, and the functional consequences of phosphorylating synaptic GABAA receptors of adult CNS neurons are poorly understood. We used whole-cell patch-clamp recordings of GABAA receptor-mediated miniature IPSCs (mIPSCs) in CA1 pyramidal neurons and dentate gyrus granule cells (GCs) of adult rat hippocampal slices to determine the effects of cAMP-dependent protein kinase (PKA) and Ca2+/phospholipiddependent protein kinase (PKC) activation on the function of synaptic GABAA receptors. The mIPSCs recorded in CA1 pyramidal cells and in GCs were differentially affected by PKA and PKC. In pyramidal cells, PKA reduced mIPSC amplitudes and enhanced the fraction of events decaying with a double exponential, whereas PKC was without effect. In contrast, in GCs PKA was ineffective, but PKC increased the peak amplitude of mIPSCs and also favored double exponential decays. Intracellular perfusion of the phosphatase inhibitor microcystin revealed that synaptic GABAA receptors of pyramidal cells, but not those of GCs, are continually phosphorylated by PKA and conversely, dephosphorylated, most likely by phosphatase 1 or 2A. This differential, brain region-specific phosphorylation of GABAA receptors may produce a wide dynamic range of inhibitory synaptic strength in these two regions of the hippocampal formation.
Key words: GABAA; miniature IPSCs; phosphorylation; receptors; PKA; PKC
Copyright © 1999 Society for Neuroscience 0270-6474/99/192674-10$05.00/0
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