 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, January 15, 1999, 19(2):674-683
Modulation of Synaptic GABAA Receptor Function by PKA and PKC in Adult Hippocampal Neurons
Pierrick Poisbeau2, Michael C. Cheney1, Michael D. Browning3, and Istvan Mody1 1 Departments of Neurology and Physiology, University of California at Los Angeles, School of Medicine, Los Angeles, California 90095, 2 Laboratoire de Neurophysiologie Cellulaire et Intégrée, Centre National de la Recherche Scientifique UMR 7519, Université Louis Pasteur, 67084 Strasbourg, France, and 3 Department of Pharmacology, University of Colorado Health Science Center, Denver, Colorado 80262
Several protein kinases are known to phosphorylate Ser/Thr residues of certain GABAA receptor subunits. Yet, the effect of phosphorylation on GABAA receptor function in neurons remains controversial, and the functional consequences of phosphorylating synaptic GABAA receptors of adult CNS neurons are poorly understood. We used whole-cell patch-clamp recordings of GABAA receptor-mediated miniature IPSCs (mIPSCs) in CA1 pyramidal neurons and dentate gyrus granule cells (GCs) of adult rat hippocampal slices to determine the effects of cAMP-dependent protein kinase (PKA) and Ca2+/phospholipiddependent protein kinase (PKC) activation on the function of synaptic GABAA receptors. The mIPSCs recorded in CA1 pyramidal cells and in GCs were differentially affected by PKA and PKC. In pyramidal cells, PKA reduced mIPSC amplitudes and enhanced the fraction of events decaying with a double exponential, whereas PKC was without effect. In contrast, in GCs PKA was ineffective, but PKC increased the peak amplitude of mIPSCs and also favored double exponential decays. Intracellular perfusion of the phosphatase inhibitor microcystin revealed that synaptic GABAA receptors of pyramidal cells, but not those of GCs, are continually phosphorylated by PKA and conversely, dephosphorylated, most likely by phosphatase 1 or 2A. This differential, brain region-specific phosphorylation of GABAA receptors may produce a wide dynamic range of inhibitory synaptic strength in these two regions of the hippocampal formation.
Key words: GABAA; miniature IPSCs; phosphorylation; receptors; PKA; PKC
Copyright © 1999 Society for Neuroscience 0270-6474/99/192674-10$05.00/0
This article has been cited by other articles:
S. Kumar, B. M. Lane, and A. L. Morrow
Differential Effects of Systemic Ethanol Administration on Protein Kinase C{epsilon}, {gamma}, and beta Isoform Expression, Membrane Translocation, and Target Phosphorylation: Reversal by Chronic Ethanol Exposure
J. Pharmacol. Exp. Ther., December 1, 2006; 319(3): 1366 - 1375.
[Abstract] [Full Text] [PDF]
B. Sahin, H. Shu, J. Fernandez, A. El-Armouche, J. D. Molkentin, A. C. Nairn, and J. A. Bibb
Phosphorylation of Protein Phosphatase Inhibitor-1 by Protein Kinase C
J. Biol. Chem., August 25, 2006; 281(34): 24322 - 24335.
[Abstract] [Full Text] [PDF]
M. M. Huntsman and J. R. Huguenard
Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor {beta}1 subunit
J. Physiol., April 15, 2006; 572(2): 459 - 475.
[Abstract] [Full Text] [PDF]
I. Mody
Aspects of the homeostaic plasticity of GABAA receptor-mediated inhibition
J. Physiol., January 1, 2005; 562(1): 37 - 46.
[Abstract] [Full Text] [PDF]
I. Aradi and G. Maccaferri
Cell Type-Specific Synaptic Dynamics of Synchronized Bursting in the Juvenile CA3 Rat Hippocampus
J. Neurosci., October 27, 2004; 24(43): 9681 - 9692.
[Abstract] [Full Text] [PDF]
J. J. Laschet, F. Minier, I. Kurcewicz, M. H. Bureau, S. Trottier, F. Jeanneteau, N. Griffon, B. Samyn, J. Van Beeumen, J. Louvel, et al.
Glyceraldehyde-3-Phosphate Dehydrogenase Is a GABAA Receptor Kinase Linking Glycolysis to Neuronal Inhibition
J. Neurosci., September 1, 2004; 24(35): 7614 - 7622.
[Abstract] [Full Text] [PDF]
C. Leroy, P. Poisbeau, A. F. Keller, and A. Nehlig
Pharmacological plasticity of GABAA receptors at dentate gyrus synapses in a rat model of temporal lobe epilepsy
J. Physiol., June 1, 2004; 557(2): 473 - 487.
[Abstract] [Full Text] [PDF]
D. J. Hinkle and R. L. Macdonald
{beta} Subunit Phosphorylation Selectively Increases Fast Desensitization and Prolongs Deactivation of {alpha}1{beta}1{gamma}2L and {alpha}1{beta}3{gamma}2L GABAA Receptor Currents
J. Neurosci., December 17, 2003; 23(37): 11698 - 11710.
[Abstract] [Full Text] [PDF]
A. B. BRUSSAARD and J.-J. KOKSMA
Conditional Regulation of Neurosteroid Sensitivity of GABAA Receptors
Ann. N.Y. Acad. Sci., December 1, 2003; 1007(1): 29 - 36.
[Abstract] [Full Text] [PDF]
L. P. LaGrange, G. M. Toney, and V. S. Bishop
Effect of Intravenous Angiotensin II Infusion on Responses to Hypothalamic PVN Injection of Bicuculline
Hypertension, December 1, 2003; 42(6): 1124 - 1129.
[Abstract] [Full Text] [PDF]
A. Bacci, U. Rudolph, J. R. Huguenard, and D. A. Prince
Major Differences in Inhibitory Synaptic Transmission onto Two Neocortical Interneuron Subclasses
J. Neurosci., October 22, 2003; 23(29): 9664 - 9674.
[Abstract] [Full Text] [PDF]
E. H. Chang, V. C. Kotak, and D. H. Sanes
Long-Term Depression of Synaptic Inhibition Is Expressed Postsynaptically in the Developing Auditory System
J Neurophysiol, September 1, 2003; 90(3): 1479 - 1488.
[Abstract] [Full Text] [PDF]
F.-C. Hsu, R. Waldeck, D. S. Faber, and S. S. Smith
Neurosteroid Effects on GABAergic Synaptic Plasticity in Hippocampus
J Neurophysiol, April 1, 2003; 89(4): 1929 - 1940.
[Abstract] [Full Text] [PDF]
J.-J. Koksma, R. E. van Kesteren, T. W. Rosahl, R. Zwart, A. B. Smit, H. Luddens, and A. B. Brussaard
Oxytocin Regulates Neurosteroid Modulation of GABAA Receptors in Supraoptic Nucleus around Parturition
J. Neurosci., February 1, 2003; 23(3): 788 - 797.
[Abstract] [Full Text] [PDF]
J. Wang, S. Liu, U. Haditsch, W. Tu, K. Cochrane, G. Ahmadian, L. Tran, J. Paw, Y. Wang, I. Mansuy, et al.
Interaction of Calcineurin and Type-A GABA Receptor gamma 2 Subunits Produces Long-Term Depression at CA1 Inhibitory Synapses
J. Neurosci., February 1, 2003; 23(3): 826 - 836.
[Abstract] [Full Text] [PDF]
B. K. Hoffpauir and E. L. Gleason
Activation of mGluR5 Modulates GABAA Receptor Function in Retinal Amacrine Cells
J Neurophysiol, October 1, 2002; 88(4): 1766 - 1776.
[Abstract] [Full Text] [PDF]
C. Henneberger, R. Juttner, T. Rothe, and R. Grantyn
Postsynaptic Action of BDNF on GABAergic Synaptic Transmission in the Superficial Layers of the Mouse Superior Colliculus
J Neurophysiol, August 1, 2002; 88(2): 595 - 603.
[Abstract] [Full Text] [PDF]
N. J. Brandon, J. N. Jovanovic, T. G. Smart, and S. J. Moss
Receptor for Activated C Kinase-1 Facilitates Protein Kinase C-Dependent Phosphorylation and Functional Modulation of GABAA Receptors with the Activation of G-Protein-Coupled Receptors
J. Neurosci., August 1, 2002; 22(15): 6353 - 6361.
[Abstract] [Full Text] [PDF]
J. M. Brundege and J. T. Williams
Differential Modulation of Nucleus Accumbens Synapses
J Neurophysiol, July 1, 2002; 88(1): 142 - 151.
[Abstract] [Full Text] [PDF]
C. Gonzalez-Islas and J. J. Hablitz
Dopamine Inhibition of Evoked IPSCs in Rat Prefrontal Cortex
J Neurophysiol, December 1, 2001; 86(6): 2911 - 2918.
[Abstract] [Full Text] [PDF]
A. F. Keller, J. A. M. Coull, N. Chery, P. Poisbeau, and Y. De Koninck
Region-Specific Developmental Specialization of GABA-Glycine Cosynapses in Laminas I-II of the Rat Spinal Dorsal Horn
J. Neurosci., October 15, 2001; 21(20): 7871 - 7880.
[Abstract] [Full Text] [PDF]
T.-p. Yu, S. McKinney, H. A. Lester, and N. Davidson
gamma -Aminobutyric acid type A receptors modulate cAMP-mediated long-term potentiation and long-term depression at monosynaptic CA3-CA1 synapses
PNAS, April 5, 2001; (2001) 91093998.
[Abstract] [Full Text]
J. Flores-Hernandez, S. Hernandez, G. L. Snyder, Z. Yan, A. A. Fienberg, S. J. Moss, P. Greengard, and D. J. Surmeier
D1 Dopamine Receptor Activation Reduces GABAA Receptor Currents in Neostriatal Neurons Through a PKA/DARPP-32/PP1 Signaling Cascade
J Neurophysiol, May 1, 2000; 83(5): 2996 - 3004.
[Abstract] [Full Text] [PDF]
A. Fancsik, D. M. Linn, and J. G. Tasker
Neurosteroid Modulation of GABA IPSCs Is Phosphorylation Dependent
J. Neurosci., May 1, 2000; 20(9): 3067 - 3075.
[Abstract] [Full Text] [PDF]
S. Wang, Q. Cheng, S. Malik, and J. Yang
Interleukin-1beta Inhibits gamma -Aminobutyric Acid Type A (GABAA) Receptor Current in Cultured Hippocampal Neurons
J. Pharmacol. Exp. Ther., February 1, 2000; 292(2): 497 - 504.
[Abstract] [Full Text]
M. I. Banks and R. A. Pearce
Kinetic Differences between Synaptic and Extrasynaptic GABAA Receptors in CA1 Pyramidal Cells
J. Neurosci., February 1, 2000; 20(3): 937 - 948.
[Abstract] [Full Text] [PDF]
M. M. Huntsman and J. R. Huguenard
Nucleus-Specific Differences in GABAA-Receptor-Mediated Inhibition Are Enhanced During Thalamic Development
J Neurophysiol, January 1, 2000; 83(1): 350 - 358.
[Abstract] [Full Text] [PDF]
E. I. Tietz, X. J. Zeng, S. Chen, S. M. Lilly, H. C. Rosenberg, and P. Kometiani
Antagonist-Induced Reversal of Functional and Structural Measures of Hippocampal Benzodiazepine Tolerance
J. Pharmacol. Exp. Ther., December 1, 1999; 291(3): 932 - 942.
[Abstract] [Full Text]
N. J. Brandon, P. Delmas, J. T. Kittler, B. J. McDonald, W. Sieghart, D. A. Brown, T. G. Smart, and S. J. Moss
GABAA Receptor Phosphorylation and Functional Modulation in Cortical Neurons by a Protein Kinase C-dependent Pathway
J. Biol. Chem., December 1, 2000; 275(49): 38856 - 38862.
[Abstract] [Full Text] [PDF]
A. B. Brussaard, J. Wossink, J. C. Lodder, and K. S. Kits
Progesterone-metabolite prevents protein kinase C-dependent modulation of gamma -aminobutyric acid type A receptors in oxytocin neurons
PNAS, March 28, 2000; 97(7): 3625 - 3630.
[Abstract] [Full Text] [PDF]
T.-p. Yu, S. McKinney, H. A. Lester, and N. Davidson
gamma -Aminobutyric acid type A receptors modulate cAMP-mediated long-term potentiation and long-term depression at monosynaptic CA3-CA1 synapses
PNAS, April 24, 2001; 98(9): 5264 - 5269.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|