 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
The Journal of Neuroscience, January 15, 1999, 19(2):716-725
Lipopolysaccharide Injected into the Cerebral Ventricle Evokes Fever through Induction of Cyclooxygenase-2 in Brain Endothelial Cells
Chunyu Cao, Kiyoshi Matsumura, Masashi Ozaki, and Yasuyoshi Watanabe Department of Neuroscience, Osaka Bioscience Institute, Furuedai 6-2-4, Suita, Osaka 565-0874, Japan
Activation of the arachidonic acid cascade is an essential step for the development of fever during brain inflammation. We investigated the brain sites where this activation takes place by use of a rat model of brain inflammation. Intracerebroventricular administration of lipopolysaccharide but not of its vehicle evoked fever. The fever was markedly suppressed when the rats had been pretreated with a cyclooxygenase-2-specific inhibitor. In situ hybridization and immunohistochemical studies revealed that cyclooxygenase-2 mRNA and its protein were induced by lipopolysaccharide in blood vessels near the cerebral ventricles and in those in the subarachnoidal space. Double immunohistochemical staining revealed that these cyclooxygenase-2-positive cells were mostly endothelial cells. The time course of fever and that of cyclooxygenase-2 induction in the endothelial cells were in parallel. Cyclooxygenase-2 mRNA in a certain type of telencephalic neurons was also upregulated by the intracerebroventricular administration, but this neuronal response occurred both in vehicle-injected rats and in lipopolysaccharide-injected ones to the same extent. Therefore, the neuronal response was not essential to the development of fever. These results suggest that brain endothelial cells play a crucial role in the development of fever during brain inflammation by activating their arachidonic acid cascade.
Key words: brain inflammation; fever; meningitis; cyclooxygenase; prostaglandin; endothelial cells
Copyright © 1999 Society for Neuroscience 0270-6474/99/192716-10$05.00/0
This article has been cited by other articles:
A. Sapirstein, H. Saito, S. J. Texel, T. A. Samad, E. O'Leary, and J. V. Bonventre
Cytosolic phospholipase A2{alpha} regulates induction of brain cyclooxygenase-2 in a mouse model of inflammation
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1774 - R1782.
[Abstract] [Full Text] [PDF]
A. S. C. Fabricio, F. H. Veiga, R. Cristofoletti, P. Navarra, and G. E. P. Souza
The effects of selective and nonselective cyclooxygenase inhibitors on endothelin-1-induced fever in rats
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2005; 288(3): R671 - R677.
[Abstract] [Full Text] [PDF]
N. P. Turrin and S. Rivest
Unraveling the Molecular Details Involved in the Intimate Link between the Immune and Neuroendocrine Systems
Experimental Biology and Medicine, November 1, 2004; 229(10): 996 - 1006.
[Abstract] [Full Text] [PDF]
K. Imai-Matsumura, K. Matsumura, A. Terao, and Y. Watanabe
Attenuated fever in pregnant rats is associated with blunted syntheses of brain cyclooxygenase-2 and PGE2
Am J Physiol Regulatory Integrative Comp Physiol, December 1, 2002; 283(6): R1346 - R1353.
[Abstract] [Full Text] [PDF]
A. Mouihate, M-S. Clerget-Froidevaux, K. Nakamura, M. Negishi, J. L. Wallace, and Q. J. Pittman
Suppression of fever at near term is associated with reduced COX-2 protein expression in rat hypothalamus
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2002; 283(3): R800 - R805.
[Abstract] [Full Text] [PDF]
K. Nakamura, K. Matsumura, T. Kaneko, S. Kobayashi, H. Katoh, and M. Negishi
The Rostral Raphe Pallidus Nucleus Mediates Pyrogenic Transmission from the Preoptic Area
J. Neurosci., June 1, 2002; 22(11): 4600 - 4610.
[Abstract] [Full Text] [PDF]
A. Ledeboer, R. Binnekade, J. J. P. Breve, J. G. J. M. Bol, F. J. H. Tilders, and A.-M. Van Dam
Site-specific modulation of LPS-induced fever and interleukin-1beta expression in rats by interleukin-10
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2002; 282(6): R1762 - R1772.
[Abstract] [Full Text] [PDF]
K. Yamagata, K. Matsumura, W. Inoue, T. Shiraki, K. Suzuki, S. Yasuda, H. Sugiura, C. Cao, Y. Watanabe, and S. Kobayashi
Coexpression of Microsomal-Type Prostaglandin E Synthase with Cyclooxygenase-2 in Brain Endothelial Cells of Rats during Endotoxin-Induced Fever
J. Neurosci., April 15, 2001; 21(8): 2669 - 2677.
[Abstract] [Full Text] [PDF]
L. G. Fischer, M. W. Hollmann, D. J. Horstman, and G. F. Rich
Cyclooxygenase Inhibitors Attenuate Bradykinin-Induced Vasoconstriction in Septic Isolated Rat Lungs
Anesth. Analg., March 1, 2000; 90(3): 625 - 631.
[Abstract] [Full Text] [PDF]
S. Rivest, S. Lacroix, L. Vallières, S. Nadeau, J. Zhang, and N. Laflamme
How the Blood Talks to the Brain Parenchyma and the Paraventricular Nucleus of the Hypothalamus During Systemic Inflammatory and Infectious Stimuli
Experimental Biology and Medicine, January 1, 2000; 223(1): 22 - 38.
[Abstract] [Full Text]
N. Laflamme, S. Lacroix, and S. Rivest
An Essential Role of Interleukin-1beta in Mediating NF-kappa B Activity and COX-2 Transcription in Cells of the Blood-Brain Barrier in Response to a Systemic and Localized Inflammation But Not During Endotoxemia
J. Neurosci., December 15, 1999; 19(24): 10923 - 10930.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|