QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (100) Citing Articles via Google Scholar Google Scholar Articles by Boehm, S. Search for Related Content PubMed PubMed Citation Articles by Boehm, S.

 Previous Article  |  Next Article 

The Journal of Neuroscience, January 15, 1999, 19(2):737-746

ATP Stimulates Sympathetic Transmitter Release via Presynaptic P2X Purinoceptors

Stefan Boehm

Department of Neuropharmacology, University of Vienna, A-1090 Vienna, Austria

ATP is a fast transmitter in sympathetic ganglia and at the sympathoeffector junction. In primary cultures of dissociated rat superior cervical ganglion neurons, ATP elicits noradrenaline release in an entirely Ca²⁺-dependent manner. Nevertheless, ATP-evoked noradrenaline release was only partially reduced (by ~50%) when either Na⁺ or Ca²⁺ channels were blocked, which indicates that ATP receptors themselves mediated transmembrane Ca²⁺ entry. An "axonal" preparation was obtained by removing ganglia from explant cultures, which left a network of neurites behind; immunostaining for axonal and dendritic markers revealed that all of these neurites were axons. In this preparation, ATP raised intraaxonal Ca²⁺ and triggered noradrenaline release, and these actions were not altered when Ca²⁺ channels were blocked by Cd²⁺. Hence, Ca²⁺-permeable ATP-gated ion channels, i.e., P2X purinoceptors, are located at presynaptic sites and directly mediate Ca²⁺-dependent transmitter release. These presynaptic P2X receptors displayed a rank order of agonist potency of ATP  2-methylthio-ATP > ATPS ,-methylene-ATP  ,-methylene-L-ATP and were blocked by suramin or PPADS. ATP, 2-methylthio-ATP, and ATPS also evoked inward currents measured at neuronal somata, but there these agonists were equipotent. Hence, presynaptic P2X receptors resemble the cloned P2X2 subtype, but they appear to differ from somatodendritic P2X receptors in terms of agonist sensitivity. Suramin reduced depolarization-evoked noradrenaline release by up to 20%, when autoinhibitory mechanisms were inactivated by pertussis toxin. These results indicate that presynaptic P2X purinoceptors mediate a positive, whereas G-protein-coupled P2Y purinoceptors mediate a negative, feedback modulation of sympathetic transmitter release.

Key words: rat superior cervical ganglion neurons; noradrenaline release; ATP; P2X receptors; presynaptic modulation; intracellular Ca²⁺

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/192737-10$05.00/0

This article has been cited by other articles:

				Z. G. Huang, K. J. S. Griffioen, X. Wang, O. Dergacheva, H. Kamendi, C. Gorini, and D. Mendelowitz Nicotinic Receptor Activation Occludes Purinergic Control of Central Cardiorespiratory Network Responses to Hypoxia/Hypercapnia J Neurophysiol, October 1, 2007; 98(4): 2429 - 2438. [Abstract] [Full Text] [PDF]

				G. Burnstock Physiology and Pathophysiology of Purinergic Neurotransmission Physiol Rev, April 1, 2007; 87(2): 659 - 797. [Abstract] [Full Text] [PDF]

				Y. Ren, X. Zou, L. Fang, and Q. Lin Involvement of Peripheral Purinoceptors in Sympathetic Modulation of Capsaicin-Induced Sensitization of Primary Afferent Fibers J Neurophysiol, November 1, 2006; 96(5): 2207 - 2216. [Abstract] [Full Text] [PDF]

				H. A. Kluess, J. B. Buckwalter, J. J. Hamann, D. S. DeLorey, and P. S. Clifford Frequency and pattern dependence of adrenergic and purinergic vasoconstriction in rat skeletal muscle arteries Exp Physiol, November 1, 2006; 91(6): 1051 - 1058. [Abstract] [Full Text] [PDF]

				L. A. P. Chaves, E. P. Pontelli, and W. A. Varanda P2X receptors in mouse Leydig cells Am J Physiol Cell Physiol, April 1, 2006; 290(4): C1009 - C1017. [Abstract] [Full Text] [PDF]

				S. G. Lechner, S. Hussl, K. W. Schicker, H. Drobny, and S. Boehm Presynaptic Inhibition via a Phospholipase C- and Phosphatidylinositol Bisphosphate-Dependent Regulation of Neuronal Ca2+ Channels Mol. Pharmacol., November 1, 2005; 68(5): 1387 - 1396. [Abstract] [Full Text] [PDF]

				T. Machida, P. M. Heerdt, A. C. Reid, U. Schafer, R. B. Silver, M. J. Broekman, A. J. Marcus, and R. Levi Ectonucleoside Triphosphate Diphosphohydrolase1/CD39, Localized in Neurons of Human and Porcine Heart, Modulates ATP-Induced Norepinephrine Exocytosis J. Pharmacol. Exp. Ther., May 1, 2005; 313(2): 570 - 577. [Abstract] [Full Text] [PDF]

				F. Saitow, T. Murakoshi, H. Suzuki, and S. Konishi Metabotropic P2Y Purinoceptor-Mediated Presynaptic and Postsynaptic Enhancement of Cerebellar GABAergic Transmission J. Neurosci., February 23, 2005; 25(8): 2108 - 2116. [Abstract] [Full Text] [PDF]

				L. Papp, T. Balazsa, A. Kofalvi, F. Erdelyi, G. Szabo, E. S. Vizi, and B. Sperlagh P2X Receptor Activation Elicits Transporter-Mediated Noradrenaline Release from Rat Hippocampal Slices J. Pharmacol. Exp. Ther., September 1, 2004; 310(3): 973 - 980. [Abstract] [Full Text] [PDF]

				J. H. Kim, J. H. Nam, M.-H. Kim, D.-S. Koh, S.-J. Choi, S. J. Kim, J. E. Lee, K. M. Min, D.-Y. Uhm, and S. J. Kim Purinergic Receptors Coupled to Intracellular Ca2+ Signals and Exocytosis in Rat Prostate Neuroendocrine Cells J. Biol. Chem., June 25, 2004; 279(26): 27345 - 27356. [Abstract] [Full Text] [PDF]

				J. A. Calvert and R. J. Evans Heterogeneity of P2X Receptors in Sympathetic Neurons: Contribution of Neuronal P2X1 Receptors Revealed Using Knockout Mice Mol. Pharmacol., January 1, 2004; 65(1): 139 - 148. [Abstract] [Full Text] [PDF]

				S. G Lechner, M. Mayer, and S. Boehm Activation of M1 muscarinic receptors triggers transmitter release from rat sympathetic neurons through an inhibition of M-type K+ channels J. Physiol., December 15, 2003; 553(3): 789 - 802. [Abstract] [Full Text] [PDF]

				G. Queiroz, C. Talaia, and J. Goncalves ATP Modulates Noradrenaline Release by Activation of Inhibitory P2Y Receptors and Facilitatory P2X Receptors in the Rat Vas Deferens J. Pharmacol. Exp. Ther., November 1, 2003; 307(2): 809 - 815. [Abstract] [Full Text] [PDF]

				J. G. Gu P2X Receptor-Mediated Modulation of Sensory Transmission to the Spinal Cord Dorsal Horn Neuroscientist, October 1, 2003; 9(5): 370 - 378. [Abstract] [PDF]

				E. Moskvina, U. Unterberger, and S. Boehm Activity-Dependent Autocrine-Paracrine Activation of Neuronal P2Y Receptors J. Neurosci., August 20, 2003; 23(20): 7479 - 7488. [Abstract] [Full Text] [PDF]

				A. J. Marcus, M. J. Broekman, J. H. F. Drosopoulos, N. Islam, D. J. Pinsky, C. Sesti, and R. Levi Metabolic Control of Excessive Extracellular Nucleotide Accumulation by CD39/Ecto-Nucleotidase-1: Implications for Ischemic Vascular Diseases J. Pharmacol. Exp. Ther., April 1, 2003; 305(1): 9 - 16. [Abstract] [Full Text]

				S. A. Masino, L. Diao, P. Illes, N. R. Zahniser, G. A. Larson, B. Johansson, B. B. Fredholm, and T. V. Dunwiddie Modulation of Hippocampal Glutamatergic Transmission by ATP Is Dependent on Adenosine A1 Receptors J. Pharmacol. Exp. Ther., October 1, 2002; 303(1): 356 - 363. [Abstract] [Full Text] [PDF]

				R. A. North Molecular Physiology of P2X Receptors Physiol Rev, October 1, 2002; 82(4): 1013 - 1067. [Abstract] [Full Text] [PDF]

				T. Scholze, E. Moskvina, M. Mayer, H. Just, H. Kubista, and S. Boehm Sympathoexcitation by Bradykinin Involves Ca2+-Independent Protein Kinase C J. Neurosci., July 15, 2002; 22(14): 5823 - 5832. [Abstract] [Full Text] [PDF]

				S. Boehm and H. Kubista Fine Tuning of Sympathetic Transmitter Release via Ionotropic and Metabotropic Presynaptic Receptors Pharmacol. Rev., March 1, 2002; 54(1): 43 - 99. [Abstract] [Full Text] [PDF]

				S. A. Deuchars, L. Atkinson, R. E. Brooke, H. Musa, C. J. Milligan, T. F. C. Batten, N. J. Buckley, S. H. Parson, and J. Deuchars Neuronal P2X7 Receptors Are Targeted to Presynaptic Terminals in the Central and Peripheral Nervous Systems J. Neurosci., September 15, 2001; 21(18): 7143 - 7152. [Abstract] [Full Text] [PDF]

				B. S. Khakh, G. Burnstock, C. Kennedy, B. F. King, R. A. North, P. Seguela, M. Voigt, and P. P. A. Humphrey International Union of Pharmacology. XXIV. Current Status of the Nomenclature and Properties of P2X Receptors and Their Subunits Pharmacol. Rev., March 1, 2001; 53(1): 107 - 118. [Abstract] [Full Text] [PDF]

				E. Bofill-Cardona, N. Vartian, C. Nanoff, M. Freissmuth, and S. Boehm Two Different Signaling Mechanisms Involved in the Excitation of Rat Sympathetic Neurons by Uridine Nucleotides Mol. Pharmacol., June 1, 2000; 57(6): 1165 - 1172. [Abstract] [Full Text]

				S. Hugel and R. Schlichter Presynaptic P2X Receptors Facilitate Inhibitory GABAergic Transmission between Cultured Rat Spinal Cord Dorsal Horn Neurons J. Neurosci., March 15, 2000; 20(6): 2121 - 2130. [Abstract] [Full Text] [PDF]

				E. S. Vizi Role of High-Affinity Receptors and Membrane Transporters in Nonsynaptic Communication and Drug Action in the Central Nervous System Pharmacol. Rev., March 1, 2000; 52(1): 63 - 90. [Abstract] [Full Text] [PDF]

				A. D. Powell, A. G. Teschemacher, and E. P. Seward P2Y Purinoceptors Inhibit Exocytosis in Adrenal Chromaffin Cells via Modulation of Voltage-Operated Calcium Channels J. Neurosci., January 15, 2000; 20(2): 606 - 616. [Abstract] [Full Text] [PDF]

				G.-H. Li, E. M. Lee, D. Blair, C. Holding, P. Poronnik, D. I. Cook, J. A. Barden, and M. R. Bennett The Distribution of P2X Receptor Clusters on Individual Neurons in Sympathetic Ganglia and Their Redistribution on Agonist Activation J. Biol. Chem., September 8, 2000; 275(37): 29107 - 29112. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help