 |
 Previous Article | Next Article 
The Journal of Neuroscience, January 15, 1999, 19(2):859-867
Peripheral Inflammation Facilitates A Fiber-Mediated Synaptic
Input to the Substantia Gelatinosa of the Adult Rat Spinal Cord
Hiroshi
Baba,
Timothy P.
Doubell, and
Clifford J.
Woolf
Neural Plasticity Research Group, Department of Anesthesia and
Critical Care, Massachusetts General Hospital and Harvard Medical
School, Boston, Massachusetts 02129
Whole-cell patch-clamp recordings were made from substantia
gelatinosa (SG) neurons in thick adult rat transverse spinal cord slices with attached dorsal roots to study changes in fast synaptic transmission induced by peripheral inflammation. In slices from naive
rats, primary afferent stimulation at A fiber intensity elicited
polysynaptic EPSCs in only 14 of 57 (25%) SG neurons. In
contrast, A fiber stimulation evoked polysynaptic EPSCs in 39 of 62 (63%) SG neurons recorded in slices from rats inflamed by an
intraplantar injection of complete Freund's adjuvant (CFA) 48 hr
earlier (p < 0.001). Although the
peripheral inflammation had no significant effect on the threshold and
conduction velocities of A, A , and C fibers recorded in dorsal
roots, the mean threshold intensity for eliciting EPSCs was
significantly lower in cells recorded from rats with inflammation
(naive: 33.2 ± 15.1 µA, n = 57; inflamed:
22.8 ± 11.3 µA, n = 62, p < 0.001), and the mean latency of EPSCs elicited
by A fiber stimulation in CFA-treated rats was significantly shorter
than that recorded from naive rats (3.3 ± 1.8 msec,
n = 36 vs 6.0 ± 3.5 msec,
n = 12; p = 0.010). A fiber
stimulation evoked polysynaptic IPSCs in 4 of 25 (16%) cells
recorded from naive rat preparations and 14 of 26 (54%) SG neurons
from CFA-treated rats (p < 0.001). The mean
threshold intensity for IPSCs was also significantly lower in
CFA-treated rats (naive: 32.5 ± 15.7 µA, n = 25; inflamed: 21.9 ± 9.9 µA, n = 26, p = 0.013). The facilitation of A fiber-mediated
input into the substantia gelatinosa after peripheral inflammation may contribute to altered sensory processing.
Key words:
inflammation; pain; dorsal horn; synaptic transmission; neural plasticity; substantia gelatinosa
Copyright © 1999 Society for Neuroscience 0270-6474/99/192859-09$05.00/0
This article has been cited by other articles:
|
|
|
|
 
G. Kato, Y. Kawasaki, K. Koga, D. Uta, M. Kosugi, T. Yasaka, M. Yoshimura, R.-R. Ji, and A. M. Strassman
Organization of Intralaminar and Translaminar Neuronal Connectivity in the Superficial Spinal Dorsal Horn
J. Neurosci.,
April 22, 2009;
29(16):
5088 - 5099.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Sandkuhler
Models and Mechanisms of Hyperalgesia and Allodynia
Physiol Rev,
April 1, 2009;
89(2):
707 - 758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. P. Cheppudira, B. M. Girard, S. E. Malley, K. C. Schutz, V. May, and M. A. Vizzard
Upregulation of vascular endothelial growth factor isoform VEGF-164 and receptors (VEGFR-2, Npn-1, and Npn-2) in rats with cyclophosphamide-induced cystitis
Am J Physiol Renal Physiol,
September 1, 2008;
295(3):
F826 - F836.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. B. Lu, K. Ballanyi, W. F. Colmers, and P. A. Smith
Neuron type-specific effects of brain-derived neurotrophic factor in rat superficial dorsal horn and their relevance to 'central sensitization'
J. Physiol.,
October 15, 2007;
584(2):
543 - 563.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Corrow and M. A. Vizzard
Phosphorylation of extracellular signal-regulated kinases in urinary bladder in rats with cyclophosphamide-induced cystitis
Am J Physiol Regulatory Integrative Comp Physiol,
July 1, 2007;
293(1):
R125 - R134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yasaka, G. Kato, H. Furue, M. H. Rashid, M. Sonohata, A. Tamae, Y. Murata, S. Masuko, and M. Yoshimura
Cell-type-specific excitatory and inhibitory circuits involving primary afferents in the substantia gelatinosa of the rat spinal dorsal horn in vitro
J. Physiol.,
June 1, 2007;
581(2):
603 - 618.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Kato, Y. Kawasaki, R.-R. Ji, and A. M. Strassman
Differential wiring of local excitatory and inhibitory synaptic inputs to islet cells in rat spinal lamina II demonstrated by laser scanning photostimulation
J. Physiol.,
May 1, 2007;
580(3):
815 - 833.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Matayoshi, N. Jiang, T. Katafuchi, K. Koga, H. Furue, T. Yasaka, T. Nakatsuka, X.-F. Zhou, Y. Kawasaki, N. Tanaka, et al.
Actions of brain-derived neurotrophic factor on spinal nociceptive transmission during inflammation in the rat
J. Physiol.,
December 1, 2005;
569(2):
685 - 695.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hong and J. W. Wiley
Early Painful Diabetic Neuropathy Is Associated with Differential Changes in the Expression and Function of Vanilloid Receptor 1
J. Biol. Chem.,
January 7, 2005;
280(1):
618 - 627.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Millis
Getting the Dog Moving After Surgery
J. Am. Anim. Hosp. Assoc.,
November 1, 2004;
40(6):
429 - 436.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kawasaki, T. Kohno, Z.-Y. Zhuang, G. J. Brenner, H. Wang, C. Van Der Meer, K. Befort, C. J. Woolf, and R.-R. Ji
Ionotropic and Metabotropic Receptors, Protein Kinase A, Protein Kinase C, and Src Contribute to C-Fiber-Induced ERK Activation and cAMP Response Element-Binding Protein Phosphorylation in Dorsal Horn Neurons, Leading to Central Sensitization
J. Neurosci.,
September 22, 2004;
24(38):
8310 - 8321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kohno, K. A Moore, H. Baba, and C. J Woolf
Peripheral nerve injury alters excitatory synaptic transmission in lamina II of the rat dorsal horn
J. Physiol.,
April 1, 2003;
548(1):
131 - 138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. G. Khasabov, S. D. Rogers, J. R. Ghilardi, C. M. Peters, P. W. Mantyh, and D. A. Simone
Spinal Neurons that Possess the Substance P Receptor Are Required for the Development of Central Sensitization
J. Neurosci.,
October 15, 2002;
22(20):
9086 - 9098.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Moore, T. Kohno, L. A. Karchewski, J. Scholz, H. Baba, and C. J. Woolf
Partial Peripheral Nerve Injury Promotes a Selective Loss of GABAergic Inhibition in the Superficial Dorsal Horn of the Spinal Cord
J. Neurosci.,
August 1, 2002;
22(15):
6724 - 6731.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Malley and M. A. Vizzard
Changes in urinary bladder cytokine mRNA and protein after cyclophosphamide-induced cystitis
Physiol Genomics,
April 10, 2002;
9(1):
5 - 13.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Magerl, P. N. Fuchs, R. A. Meyer, and R.-D. Treede
Roles of capsaicin-insensitive nociceptors in cutaneous pain and secondary hyperalgesia
Brain,
September 1, 2001;
124(9):
1754 - 1764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fitzgerald and S. Beggs
Book Review: The Neurobiology of Pain: Developmental Aspects
Neuroscientist,
June 1, 2001;
7(3):
246 - 257.
[Abstract]
[PDF]
|
|
|
|
|
|
|
H. Baba, T. Kohno, K. A. Moore, and C. J. Woolf
Direct Activation of Rat Spinal Dorsal Horn Neurons by Prostaglandin E2
J. Neurosci.,
March 1, 2001;
21(5):
1750 - 1756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G.-Y. Xu, L.-Y. M. Huang, and Z.-Q. Zhao
Activation of silent mechanoreceptive cat C and A{delta} sensory neurons and their substance P expression following peripheral inflammation
J. Physiol.,
October 15, 2000;
528(2):
339 - 348.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ito, E. Kumamoto, M. Takeda, M. Takeda, K. Shibata, H. Sagai, and M. Yoshimura
Mechanisms for Ovariectomy-Induced Hyperalgesia and Its Relief by Calcitonin: Participation of 5-HT1A-Like Receptor on C-Afferent Terminals in Substantia Gelatinosa of the Rat Spinal Cord
J. Neurosci.,
August 15, 2000;
20(16):
6302 - 6308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Kohama, K. Ishikawa, and J. D. Kocsis
Synaptic Reorganization in the Substantia Gelatinosa After Peripheral Nerve Neuroma Formation: Aberrant Innervation of Lamina II Neurons by Abeta Afferents
J. Neurosci.,
February 15, 2000;
20(4):
1538 - 1549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Baba, T. P. Doubell, K. A. Moore, and C. J. Woolf
Silent NMDA Receptor-Mediated Synapses Are Developmentally Regulated in the Dorsal Horn of the Rat Spinal Cord
J Neurophysiol,
February 1, 2000;
83(2):
955 - 962.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. E. Malley and M. A. Vizzard
Changes in urinary bladder cytokine mRNA and protein after cyclophosphamide-induced cystitis
Physiol Genomics,
April 10, 2002;
9(1):
5 - 13.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
