QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (43) Citing Articles via Google Scholar Google Scholar Articles by DeZazzo, J. Articles by Tully, T. Search for Related Content PubMed PubMed Citation Articles by DeZazzo, J. Articles by Tully, T.

 Previous Article  |  Next Article 

The Journal of Neuroscience, October 15, 1999, 19(20):8740-8746

Developmental Expression of an amn⁺ Transgene Rescues the Mutant Memory Defect of amnesiac Adults

James DeZazzo, Shouzhen Xia, Jeff Christensen, Klara Velinzon, and Tim Tully

Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724

The Drosophila memory gene amnesiac (amn) has been proposed to encode a neuropeptide protein, which includes regions homologous to vertebrate pituitary adenylyl cyclase-activating peptide (PACAP; Feany and Quinn, 1995). Definitive experiments to link this gene to memory formation, however, have not yet been accomplished (Kandel and Abel, 1995). The experiments described here demonstrate that the putative amn transcript is involved in adult memory formation. With the use of a UAS-amn⁺ transgene, we show complete rescue of memory defects in amn^28A, a mutant allele caused by the insertion of a GAL4 enhancer trap transposon (Moore et al., 1998). Study of the amn^28A reporter reveals widespread expression in the adult brain but also enriched expression in the embryonic and larval nervous systems. To begin addressing the temporal requirement of amn in memory, we asked whether the memory defects could be rescued by restricting transgenic expression to the adult stage. A heat-shock regimen shown previously to rescue fully the amn ethanol sensitivity defect (Moore et al., 1998) failed to rescue the memory defect. These results, coupled with previous genetic and anatomical studies, suggest that adult memory formation and ethanol sensitivity have different temporal and spatial requirements for amn.

Key words: Drosophila; neuropeptide; Pavlovian learning; neurogenetics; mutants; behavioral rescue; olfactory memory; associative learning; neurodevelopment; cAMP signaling; ethanol sensitivity

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19208740-07$05.00/0

This article has been cited by other articles:

				R. Menzel and G. Manz Neural plasticity of mushroom body-extrinsic neurons in the honeybee brain J. Exp. Biol., November 15, 2005; 208(22): 4317 - 4332. [Abstract] [Full Text] [PDF]

				K. Honjo and K. Furukubo-Tokunaga Induction of cAMP Response Element-Binding Protein-Dependent Medium-Term Memory by Appetitive Gustatory Reinforcement in Drosophila Larvae J. Neurosci., August 31, 2005; 25(35): 7905 - 7913. [Abstract] [Full Text] [PDF]

				Y.-q. Wang, Y.-p. Qian, S. Yang, H. Shi, C.-h. Liao, H.-K. Zheng, J. Wang, A. A. Lin, L. L. Cavalli-Sforza, P. A. Underhill, et al. Accelerated Evolution of the Pituitary Adenylate Cyclase-Activating Polypeptide Precursor Gene During Human Origin Genetics, June 1, 2005; 170(2): 801 - 806. [Abstract] [Full Text] [PDF]

				A. Bhattacharya, S. S. Lakhman, and S. Singh Modulation of L-type Calcium Channels in Drosophila via a Pituitary Adenylyl Cyclase-activating Polypeptide (PACAP)-mediated Pathway J. Biol. Chem., September 3, 2004; 279(36): 37291 - 37297. [Abstract] [Full Text] [PDF]

				Y. Wang and Y. Zhong TARGETing "When" and "Where" Sci. Signal., February 17, 2004; 2004(220): pe5 - pe5. [Abstract] [Full Text] [PDF]

				A. R. Rodan, J. A. Kiger Jr, and U. Heberlein Functional Dissection of Neuroanatomical Loci Regulating Ethanol Sensitivity in Drosophila J. Neurosci., November 1, 2002; 22(21): 9490 - 9501. [Abstract] [Full Text] [PDF]

				N. Philip, S. F. Acevedo, and E. M. C. Skoulakis Conditional Rescue of Olfactory Learning and Memory Defects in Mutants of the 14-3-3zeta Gene leonardo J. Neurosci., November 1, 2001; 21(21): 8417 - 8425. [Abstract] [Full Text] [PDF]

				D. Vaudry, B. J. Gonzalez, M. Basille, L. Yon, A. Fournier, and H. Vaudry Pituitary Adenylate Cyclase-Activating Polypeptide and Its Receptors: From Structure to Functions Pharmacol. Rev., June 1, 2000; 52(2): 269 - 324. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help