QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (28) Citing Articles via Google Scholar Google Scholar Articles by Chyb, S. Articles by Hardie, R. C. Search for Related Content PubMed PubMed Citation Articles by Chyb, S. Articles by Hardie, R. C. Pubmed/NCBI databases Gene GEO Profiles HomoloGene UniGene Compound via MeSH Substance via MeSH

 Previous Article  |  Next Article 

The Journal of Neuroscience, October 15, 1999, 19(20):8799-8807

Modulation of the Light Response by cAMP in Drosophila Photoreceptors

Sylwester Chyb^{1, 2}, Wulf Hevers³, Michael Forte⁴, William J. Wolfgang⁴, Zvi Selinger⁵, and Roger C. Hardie^{1, 3}

Departments of ¹ Anatomy, ² Biochemistry, and ³ Zoology, Cambridge University, Cambridge CB2 3DY, United Kingdom, ⁴ Oregon Health Sciences University, Vollum Institute, Portland, Oregon 97201, and ⁵ Department of Biological Chemistry, Institute of Life Sciences, Hebrew University, Jerusalem, 91904, Israel

Phototransduction in Drosophila is mediated by a G-protein-coupled phospholipase C transduction cascade in which each absorbed photon generates a discrete electrical event, the quantum bump. In whole-cell voltage-clamp recordings, cAMP, as well as its nonhydrolyzable and membrane-permeant analogs 8-bromo-cAMP (8-Br-cAMP) and dibutyryl-cAMP, slowed down the macroscopic light response by increasing quantum bump latency, without changes in bump amplitude or duration. In contrast, cGMP or 8-Br-cGMP had no effect on light response amplitude or kinetics. None of the cyclic nucleotides activated any channels in the plasma membrane. The effects of cAMP were mimicked by application of the non-specific phosphodiesterase inhibitor IBMX and the adenylyl cyclase activator forskolin; zaprinast, a specific cGMP-phosphodiesterase inhibitor, was ineffective. Bump latency was also increased by targeted expression of either an activated G_s  subunit, which increased endogenous adenylyl cyclase activity, or an activated catalytic protein kinase A (PKA) subunit. The action of IBMX was blocked by pretreatment with the PKA inhibitor H-89. The effects of cAMP were abolished in mutants of the ninaC gene, suggesting this nonconventional myosin as a possible target for PKA-mediated phosphorylation. Dopamine (10 µM) and octopamine (100 µM) mimicked the effects of cAMP. These results indicate the existence of a G-protein-coupled adenylyl cyclase pathway in Drosophila photoreceptors, which modulates the phospholipase C-based phototransduction cascade.

Key words: phototransduction; neuromodulation; TRP; photoreceptor; dopamine; calcium; cross-talk; adenylyl cyclase; phospholipase C

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19208799-09$05.00/0

This article has been cited by other articles:

				N. Slepko, A. M. Bhattacharyya, G. R. Jackson, J. S. Steffan, J. L. Marsh, L. M. Thompson, and R. Wetzel Normal-repeat-length polyglutamine peptides accelerate aggregation nucleation and cytotoxicity of expanded polyglutamine proteins PNAS, September 26, 2006; 103(39): 14367 - 14372. [Abstract] [Full Text] [PDF]

				T.-K. Sang, C. Li, W. Liu, A. Rodriguez, J. M. Abrams, S. L. Zipursky, and G. R. Jackson Inactivation of Drosophila Apaf-1 related killer suppresses formation of polyglutamine aggregates and blocks polyglutamine pathogenesis Hum. Mol. Genet., February 1, 2005; 14(3): 357 - 372. [Abstract] [Full Text] [PDF]

				K.-i. Kimura, A. Kodama, Y. Hayasaka, and T. Ohta Activation of the cAMP/PKA signaling pathway is required for post-ecdysial cell death in wing epidermal cells of Drosophila melanogaster Development, April 1, 2004; 131(7): 1597 - 1606. [Abstract] [Full Text] [PDF]

				J. K. Inlow and L. L. Restifo Molecular and Comparative Genetics of Mental Retardation Genetics, February 1, 2004; 166(2): 835 - 881. [Abstract] [Full Text] [PDF]

				U. B. Kaupp and R. Seifert Cyclic Nucleotide-Gated Ion Channels Physiol Rev, July 1, 2002; 82(3): 769 - 824. [Abstract] [Full Text] [PDF]

				M. Fuse and J. W. Truman Modulation of ecdysis in the moth Manduca sexta: the roles of the suboesophageal and thoracic ganglia J. Exp. Biol., April 15, 2002; 205(8): 1047 - 1058. [Abstract] [Full Text] [PDF]

				A. Vehovszky and C. J. H. Elliott Activation and Reconfiguration of Fictive Feeding by the OctopamineContaining Modulatory OC Interneurons in the Snail Lymnaea J Neurophysiol, August 1, 2001; 86(2): 792 - 808. [Abstract] [Full Text] [PDF]

				W. J. Wolfgang, A. Hoskote, I. J. H. Roberts, S. Jackson, and M. Forte Genetic Analysis of the Drosophila Gs{{alpha}} Gene Genetics, July 1, 2001; 158(3): 1189 - 1201. [Abstract] [Full Text] [PDF]

				K. Venkatesh, G. Siddhartha, R. Joshi, S. Patel, and G. Hasan Interactions Between the Inositol 1,4,5-Trisphosphate and Cyclic AMP Signaling Pathways Regulate Larval Molting in Drosophila Genetics, May 1, 2001; 158(1): 309 - 318. [Abstract] [Full Text]

				S. K. Park, S. A. Sedore, C. Cronmiller, and J. Hirsh Type II cAMP-dependent Protein Kinase-deficient Drosophila Are Viable but Show Developmental, Circadian, and Drug Response Phenotypes J. Biol. Chem., June 30, 2000; 275(27): 20588 - 20596. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help