QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (153) Citing Articles via Google Scholar Google Scholar Articles by Niederöst, B. P. Articles by Bandtlow, C. E. Search for Related Content PubMed PubMed Citation Articles by Niederöst, B. P. Articles by Bandtlow, C. E.

 Previous Article  |  Next Article 

The Journal of Neuroscience, October 15, 1999, 19(20):8979-8989

Bovine CNS Myelin Contains Neurite Growth-Inhibitory Activity Associated with Chondroitin Sulfate Proteoglycans

Barbara P. Niederöst¹, Dieter R. Zimmermann², Martin E. Schwab¹, and Christine E. Bandtlow¹

¹ Brain Research Institute, University of Zürich and Swiss Federal Institute of Technology, Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland, and ² Molecular Biology Laboratory, Department of Pathology, University Hospital, Schmelzbergstrasse 12, CH-8091 Zürich, Switzerland

The absence of fiber regrowth in the injured mammalian CNS is influenced by several different factors and mechanisms. Besides the nonconducive properties of the glial scar tissue that forms around the lesion site, individual molecules present in CNS myelin and expressed by oligodendrocytes, such as NI-35/NI-250, bNI-220, and myelin-associated glycoprotein (MAG), have been isolated and shown to inhibit axonal growth. Here, we report an additional neurite growth-inhibitory activity purified from bovine spinal cord myelin that is not related to bNI-220 or MAG. This activity can be ascribed to the presence of two chondroitin sulfate proteoglycans (CSPGs), brevican and the brain-specific versican V2 splice variant. Neurite outgrowth of neonatal cerebellar granule cells and of dorsal root ganglion neurons in vitro was strongly inhibited by this myelin fraction enriched in CSPGs. Immunohistochemical staining revealed that brevican and versican V2 are present on the surfaces of differentiated oligodendrocytes. We provide evidence that treatment of oligodendrocytes with the proteoglycan synthesis inhibitors -xylosides can strongly influence the growth permissiveness of oligodendrocytes. -Xylosides abolished cell surface presentation of brevican and versican V2 and reversed growth cone collapse in encounters with oligodendrocytes as demonstrated by time-lapse video microscopy. Instead, growth cones were able to grow along or even into the processes of oligodendrocytes. Our results strongly suggest that brevican and versican V2 are additional components of CNS myelin that contribute to its nonpermissive substrate properties for axonal growth. Expression of these CSPGs on oligodendrocytes may indicate that they participate in the restriction of structural plasticity and regeneration in the adult CNS.

Key words: neurite growth inhibition; CNS myelin; chondroitin sulfate proteoglycans; oligodendrocyte; regeneration; spinal cord

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19208979-11$05.00/0

This article has been cited by other articles:

				Z. Zhang, X. Xu, Y. Zhang, J. Zhou, Z. Yu, and C. He LINGO-1 Interacts with WNK1 to Regulate Nogo-induced Inhibition of Neurite Extension J. Biol. Chem., June 5, 2009; 284(23): 15717 - 15728. [Abstract] [Full Text] [PDF]

				H.T. Ghashghaei, J. M. Weimer, R. S. Schmid, Y. Yokota, K. D. McCarthy, B. Popko, and E.S. Anton Reinduction of ErbB2 in astrocytes promotes radial glial progenitor identity in adult cerebral cortex Genes & Dev., December 15, 2007; 21(24): 3258 - 3271. [Abstract] [Full Text] [PDF]

				A. Erturk, F. Hellal, J. Enes, and F. Bradke Disorganized Microtubules Underlie the Formation of Retraction Bulbs and the Failure of Axonal Regeneration J. Neurosci., August 22, 2007; 27(34): 9169 - 9180. [Abstract] [Full Text] [PDF]

				C. Schachtrup, P. Lu, L. L. Jones, J. K. Lee, J. Lu, B. D. Sachs, B. Zheng, and K. Akassoglou Fibrinogen inhibits neurite outgrowth via beta3 integrin-mediated phosphorylation of the EGF receptor PNAS, July 10, 2007; 104(28): 11814 - 11819. [Abstract] [Full Text] [PDF]

				B. P Liu, W. B.J Cafferty, S. O Budel, and S. M Strittmatter Extracellular regulators of axonal growth in the adult central nervous system Phil Trans R Soc B, September 29, 2006; 361(1473): 1593 - 1610. [Abstract] [Full Text] [PDF]

				I. C Maier and M. E Schwab Sprouting, regeneration and circuit formation in the injured spinal cord: factors and activity Phil Trans R Soc B, September 29, 2006; 361(1473): 1611 - 1634. [Abstract] [Full Text] [PDF]

				K. Nakanishi, S. Aono, K. Hirano, Y. Kuroda, M. Ida, Y. Tokita, F. Matsui, and A. Oohira Identification of Neurite Outgrowth-promoting Domains of Neuroglycan C, a Brain-specific Chondroitin Sulfate Proteoglycan, and Involvement of Phosphatidylinositol 3-Kinase and Protein Kinase C Signaling Pathways in Neuritogenesis J. Biol. Chem., August 25, 2006; 281(34): 24970 - 24978. [Abstract] [Full Text] [PDF]

				Y.-Y. Xiang, H. Dong, Y. Wan, J. Li, A. Yee, B. B. Yang, and W.-Y. Lu Versican G3 Domain Regulates Neurite Growth and Synaptic Transmission of Hippocampal Neurons by Activation of Epidermal Growth Factor Receptor J. Biol. Chem., July 14, 2006; 281(28): 19358 - 19368. [Abstract] [Full Text] [PDF]

				A. M. Tan, M. Colletti, A. T. Rorai, J. H. P. Skene, and J. M. Levine Antibodies against the NG2 proteoglycan promote the regeneration of sensory axons within the dorsal columns of the spinal cord. J. Neurosci., May 3, 2006; 26(18): 4729 - 4739. [Abstract] [Full Text] [PDF]

				A. Logan, Z. Ahmed, A. Baird, A. M. Gonzalez, and M. Berry Neurotrophic factor synergy is required for neuronal survival and disinhibited axon regeneration after CNS injury Brain, February 1, 2006; 129(2): 490 - 502. [Abstract] [Full Text] [PDF]

				J. K. Huang, G. R. Phillips, A. D. Roth, L. Pedraza, W. Shan, W. Belkaid, S. Mi, A. Fex-Svenningsen, L. Florens, J. R. Yates III, et al. Glial Membranes at the Node of Ranvier Prevent Neurite Outgrowth Science, December 16, 2005; 310(5755): 1813 - 1817. [Abstract] [Full Text] [PDF]

				M. Yamagata and J. R. Sanes Versican in the Developing Brain: Lamina-Specific Expression in Interneuronal Subsets and Role in Presynaptic Maturation J. Neurosci., September 14, 2005; 25(37): 8457 - 8467. [Abstract] [Full Text] [PDF]

				M. D. Benson, M. I. Romero, M. E. Lush, Q. R. Lu, M. Henkemeyer, and L. F. Parada Ephrin-B3 is a myelin-based inhibitor of neurite outgrowth PNAS, July 26, 2005; 102(30): 10694 - 10699. [Abstract] [Full Text] [PDF]

				K. J. M. Marler, R. Kozma, S. Ahmed, J.-M. Dong, C. Hall, and L. Lim Outgrowth of Neurites from NIE-115 Neuroblastoma Cells Is Prevented on Repulsive Substrates through the Action of PAK Mol. Cell. Biol., June 15, 2005; 25(12): 5226 - 5241. [Abstract] [Full Text] [PDF]

				E.-E. Govek, S. E. Newey, and L. Van Aelst The role of the Rho GTPases in neuronal development Genes & Dev., January 1, 2005; 19(1): 1 - 49. [Abstract] [Full Text] [PDF]

				A. R. Walmsley, G. McCombie, U. Neumann, D. Marcellin, R. Hillenbrand, A. K. Mir, and S. Frentzel Zinc metalloproteinase-mediated cleavage of the human Nogo-66 receptor J. Cell Sci., September 1, 2004; 117(19): 4591 - 4602. [Abstract] [Full Text] [PDF]

				M. Hikino, T. Mikami, A. Faissner, A.-C. E. S. Vilela-Silva, M. S. G. Pavao, and K. Sugahara Oversulfated Dermatan Sulfate Exhibits Neurite Outgrowth-promoting Activity toward Embryonic Mouse Hippocampal Neurons: IMPLICATIONS OF DERMATAN SULFATE IN NEURITOGENESIS IN THE BRAIN J. Biol. Chem., October 31, 2003; 278(44): 43744 - 43754. [Abstract] [Full Text] [PDF]

				K. Tanabe, I. Bonilla, J. A. Winkles, and S. M. Strittmatter Fibroblast Growth Factor-Inducible-14 Is Induced in Axotomized Neurons and Promotes Neurite Outgrowth J. Neurosci., October 22, 2003; 23(29): 9675 - 9686. [Abstract] [Full Text] [PDF]

				T. Oertle, M. E. van der Haar, C. E. Bandtlow, A. Robeva, P. Burfeind, A. Buss, A. B. Huber, M. Simonen, L. Schnell, C. Brosamle, et al. Nogo-A Inhibits Neurite Outgrowth and Cell Spreading with Three Discrete Regions J. Neurosci., July 2, 2003; 23(13): 5393 - 5406. [Abstract] [Full Text] [PDF]

				A. E. Fournier, B. T. Takizawa, and S. M. Strittmatter Rho Kinase Inhibition Enhances Axonal Regeneration in the Injured CNS J. Neurosci., February 15, 2003; 23(4): 1416 - 1423. [Abstract] [Full Text] [PDF]

				B. Niederost, T. Oertle, J. Fritsche, R. A. McKinney, and C. E. Bandtlow Nogo-A and Myelin-Associated Glycoprotein Mediate Neurite Growth Inhibition by Antagonistic Regulation of RhoA and Rac1 J. Neurosci., December 1, 2002; 22(23): 10368 - 10376. [Abstract] [Full Text] [PDF]

				C. Brakebusch, C. I. Seidenbecher, F. Asztely, U. Rauch, H. Matthies, H. Meyer, M. Krug, T. M. Bockers, X. Zhou, M. R. Kreutz, et al. Brevican-Deficient Mice Display Impaired Hippocampal CA1 Long-Term Potentiation but Show No Obvious Deficits in Learning and Memory Mol. Cell. Biol., November 1, 2002; 22(21): 7417 - 7427. [Abstract] [Full Text] [PDF]

				A. E. Fournier, G. C. Gould, B. P. Liu, and S. M. Strittmatter Truncated Soluble Nogo Receptor Binds Nogo-66 and Blocks Inhibition of Axon Growth by Myelin J. Neurosci., October 15, 2002; 22(20): 8876 - 8883. [Abstract] [Full Text] [PDF]

				B. K. Kwon, J. F. Borisoff, and W. Tetzlaff Molecular Targets for Therapeutic Intervention after Spinal Cord Injury Mol. Interv., July 1, 2002; 2(4): 244 - 258. [Abstract] [Full Text] [PDF]

				L. L. Jones and M. H. Tuszynski Spinal Cord Injury Elicits Expression of Keratan Sulfate Proteoglycans by Macrophages, Reactive Microglia, and Oligodendrocyte Progenitors J. Neurosci., June 1, 2002; 22(11): 4611 - 4624. [Abstract] [Full Text] [PDF]

				B. Grimpe, S. Dong, C. Doller, K. Temple, A. T. Malouf, and J. Silver The Critical Role of Basement Membrane-Independent Laminin gamma 1 Chain during Axon Regeneration in the CNS J. Neurosci., April 15, 2002; 22(8): 3144 - 3160. [Abstract] [Full Text] [PDF]

				R. A. Asher, D. A. Morgenstern, M. C. Shearer, K. H. Adcock, P. Pesheva, and J. W. Fawcett Versican Is Upregulated in CNS Injury and Is a Product of Oligodendrocyte Lineage Cells J. Neurosci., March 15, 2002; 22(6): 2225 - 2236. [Abstract] [Full Text] [PDF]

				S. David and S. S. Ousman Book Review: Recruiting the Immune Response to Promote Axon Regeneration in the Injured Spinal Cord Neuroscientist, February 1, 2002; 8(1): 33 - 41. [Abstract] [PDF]

				C. G. Becker and T. Becker Repellent Guidance of Regenerating Optic Axons by Chondroitin Sulfate Glycosaminoglycans in Zebrafish J. Neurosci., February 1, 2002; 22(3): 842 - 853. [Abstract] [Full Text] [PDF]

				J. Corcoran, P.-L. So, R. D. Barber, K. J. Vincent, N. D. Mazarakis, K. A. Mitrophanous, S. M. Kingsman, and M. Maden Retinoic acid receptor {beta}2 and neurite outgrowth in the adult mouse spinal cord in vitro J. Cell Sci., January 10, 2002; 115(19): 3779 - 3786. [Abstract] [Full Text] [PDF]

				H. Ichijo and I. Kawabata Roles of the Telencephalic Cells and their Chondroitin Sulfate Proteoglycans in Delimiting an Anterior Border of the Retinal Pathway J. Neurosci., December 1, 2001; 21(23): 9304 - 9314. [Abstract] [Full Text] [PDF]

				T. Grandpre and S. M. Strittmatter Nogo: A Molecular Determinant of Axonal Growth and Regeneration Neuroscientist, October 1, 2001; 7(5): 377 - 386. [Abstract] [PDF]

				C. E. Bandtlow and D. R. Zimmermann Proteoglycans in the Developing Brain: New Conceptual Insights for Old Proteins Physiol Rev, October 1, 2000; 80(4): 1267 - 1290. [Abstract] [Full Text] [PDF]

				M Schmalfeldt, C. Bandtlow, M. Dours-Zimmermann, K. Winterhalter, and D. Zimmermann Brain derived versican V2 is a potent inhibitor of axonal growth J. Cell Sci., January 3, 2000; 113(5): 807 - 816. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help