QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (36) Citing Articles via Google Scholar Google Scholar Articles by Brorson, J. R. Articles by Vandenberghe, W. Search for Related Content PubMed PubMed Citation Articles by Brorson, J. R. Articles by Vandenberghe, W.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 1999, 19(21):9149-9159

Ca²⁺ Permeation of AMPA Receptors in Cerebellar Neurons Expressing Glu Receptor 2

James R. Brorson, Zehui Zhang, and Wim Vandenberghe

Department of Neurology and Committees on Neurobiology and Cell Physiology, The University of Chicago, Chicago, Illinois 60637

AMPA receptors in cultured cerebellar neurons were characterized by whole-cell electrophysiological studies and single cell PCR-based quantitation of subunit mRNA expression. Purkinje neurons consistently expressed high levels of Glu receptor 2 (GluR2) mRNA and AMPA receptors with low but nonzero Ca²⁺ permeability. Other cerebellar neurons expressed AMPA receptors with a wide range of Ca²⁺ permeability and of fractional GluR2. These properties correlated on a cell-by-cell basis. Their relationship was well fit by a model that assumed stochastic assembly of subunits and GluR2 dominance in controlling divalent cation permeation, suggesting that AMPA receptor properties in individual neurons may be determined primarily by relative levels of subunit transcription. A fraction of receptors, lacking GluR2, can contribute a highly Ca²⁺-permeable component to AMPA receptor responses, even in cells expressing GluR2.

Key words: excitotoxicity; glutamate; AMPA; transcription; permeability; receptor assembly

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19219149-11$05.00/0

This article has been cited by other articles:

				N. P. Whitney, H. Peng, N. B. Erdmann, C. Tian, D. T. Monaghan, and J. C. Zheng Calcium-permeable AMPA receptors containing Q/R-unedited GluR2 direct human neural progenitor cell differentiation to neurons FASEB J, August 1, 2008; 22(8): 2888 - 2900. [Abstract] [Full Text] [PDF]

				J. Shin, F. Shen, and J. Huguenard PKC and polyamine modulation of GluR2-deficient AMPA receptors in immature neocortical pyramidal neurons of the rat J. Physiol., June 1, 2007; 581(2): 679 - 691. [Abstract] [Full Text] [PDF]

				M. M. Huntsman and J. R. Huguenard Fast IPSCs in rat thalamic reticular nucleus require the GABAA receptor {beta}1 subunit J. Physiol., April 15, 2006; 572(2): 459 - 475. [Abstract] [Full Text] [PDF]

				J. R. Brorson, D. Li, and T. Suzuki Selective Expression of Heteromeric AMPA Receptors Driven by Flip-Flop Differences J. Neurosci., April 7, 2004; 24(14): 3461 - 3470. [Abstract] [Full Text] [PDF]

				Q. Liu and M. T. T. Wong-Riley Postnatal expression of neurotransmitters, receptors, and cytochrome oxidase in the rat pre-Botzinger complex J Appl Physiol, March 1, 2002; 92(3): 923 - 934. [Abstract] [Full Text] [PDF]

				K. Iihara, D. T. Joo, J. Henderson, R. Sattler, F. A. Taverna, S. Lourensen, B. A. Orser, J. C. Roder, and M. Tymianski The Influence of Glutamate Receptor 2 Expression on Excitotoxicity in GluR2 Null Mutant Mice J. Neurosci., April 1, 2001; 21(7): 2224 - 2239. [Abstract] [Full Text] [PDF]

				P. L. Follett, P. A. Rosenberg, J. J. Volpe, and F. E. Jensen NBQX Attenuates Excitotoxic Injury in Developing White Matter J. Neurosci., December 15, 2000; 20(24): 9235 - 9241. [Abstract] [Full Text] [PDF]

				W. Vandenberghe, E. C. Ihle, D. K. Patneau, W. Robberecht, and J. R. Brorson AMPA Receptor Current Density, Not Desensitization, Predicts Selective Motoneuron Vulnerability J. Neurosci., October 1, 2000; 20(19): 7158 - 7166. [Abstract] [Full Text] [PDF]

				W. Vandenberghe, W. Robberecht, and J. R. Brorson AMPA Receptor Calcium Permeability, GluR2 Expression, and Selective Motoneuron Vulnerability J. Neurosci., January 1, 2000; 20(1): 123 - 132. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help