QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (29) Citing Articles via Google Scholar Google Scholar Articles by Giovannucci, D. R. Articles by Stuenkel, E. L. Search for Related Content PubMed PubMed Citation Articles by Giovannucci, D. R. Articles by Stuenkel, E. L. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CADMIUM COMPOUNDS CADMIUM, ELEMENTAL CALCIUM COMPOUNDS CALCIUM, ELEMENTAL

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 1999, 19(21):9261-9270

Mitochondria Regulate the Ca²⁺-Exocytosis Relationship of Bovine Adrenal Chromaffin Cells

David R. Giovannucci¹, Michael D. Hlubek², and Edward L. Stuenkel¹

Departments of ¹ Physiology and ² Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109-0622

The present study expands the contemporary view of mitochondria as important participants in cellular Ca²⁺ dynamics and provides evidence that mitochondria regulate the supply of release-competent secretory granules. Using pharmacological probes to inhibit mitochondrial Ca²⁺ import, the ability of mitochondria to modulate secretory activity in single, patch-clamped bovine chromaffin cells was examined by simultaneously monitoring rapid changes in membrane surface area (C_m) and cytosolic Ca²⁺ levels ([Ca²⁺]_c). Repetitive step depolarizations or action potential waveforms were found to raise the [Ca²⁺]_c of chromaffin cells into the 1 µM to tens of micromolar range. Inhibiting mitochondria by treatment with carbonyl cyanide p-(trifuoro-methoxy)phenylhydrazone, antimycin-oligomycin, or ruthenium red revealed that mitochondria are a prominent component for the clearance of Ca²⁺ that entered via voltage-activated Ca²⁺ channels. Disruption of cellular Ca²⁺ homeostasis by poisoning mitochondria enhanced the secretory responsiveness of chromaffin cells by increasing the amplitude of the transient rise and the time course of recovery to baseline of the evoked [Ca²⁺]_c. The enhancement of the secretory response was represented by significant deviation of the Ca²⁺-exocytosis relationship from a standard relationship that equates Ca²⁺ influx and C_m. Thus, mitochondria would play a critical role in the control of secretory activity in chromaffin cells that undergo tonic or repetitive depolarizing activity, likely by limiting the Ca²⁺-dependent activation of specific proteins that recruit or prime secretory granules for exocytosis.

Key words: membrane capacitance; exocytosis; FCCP; fura-2; furaptra; readily releasable pool

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19219261-10$05.00/0

This article has been cited by other articles:

				J. E. Geiger and N. S. Magoski Ca2+-Induced Ca2+ Release in Aplysia Bag Cell Neurons Requires Interaction Between Mitochondrial and Endoplasmic Reticulum Stores J Neurophysiol, July 1, 2008; 100(1): 24 - 37. [Abstract] [Full Text] [PDF]

				L. Vay, E. Hernandez-SanMiguel, J. Santo-Domingo, C. D. Lobaton, A. Moreno, M. Montero, and J. Alvarez Modulation of Ca2+ release and Ca2+ oscillations in HeLa cells and fibroblasts by mitochondrial Ca2+ uniporter stimulation J. Physiol., April 1, 2007; 580(1): 39 - 49. [Abstract] [Full Text] [PDF]

				A. G. Garcia, A. M. Garcia-De-Diego, L. Gandia, R. Borges, and J. Garcia-Sancho Calcium signaling and exocytosis in adrenal chromaffin cells. Physiol Rev, October 1, 2006; 86(4): 1093 - 1131. [Abstract] [Full Text] [PDF]

				I Cuchillo-Ibanez, T Lejen, A Albillos, S. D Rose, R Olivares, M Villarroya, A. G Garcia, and J.-M Trifaro Mitochondrial calcium sequestration and protein kinase C cooperate in the regulation of cortical F-actin disassembly and secretion in bovine chromaffin cells J. Physiol., October 1, 2004; 560(1): 63 - 76. [Abstract] [Full Text] [PDF]

				E. A. Jonas, D. Hoit, J. A. Hickman, T. A. Brandt, B. M. Polster, Y. Fannjiang, E. McCarthy, M. K. Montanez, J. M. Hardwick, and L. K. Kaczmarek Modulation of Synaptic Transmission by the BCL-2 Family Protein BCL-xL J. Neurosci., September 10, 2003; 23(23): 8423 - 8431. [Abstract] [Full Text] [PDF]

				R. D. Burgoyne and A. Morgan Secretory Granule Exocytosis Physiol Rev, April 1, 2003; 83(2): 581 - 632. [Abstract] [Full Text] [PDF]

				B. Billups and I. D. Forsythe Presynaptic Mitochondrial Calcium Sequestration Influences Transmission at Mammalian Central Synapses J. Neurosci., July 15, 2002; 22(14): 5840 - 5847. [Abstract] [Full Text] [PDF]

				B. T. Chen, M. V. Avshalumov, and M. E. Rice H2O2 Is a Novel, Endogenous Modulator of Synaptic Dopamine Release J Neurophysiol, June 1, 2001; 85(6): 2468 - 2476. [Abstract] [Full Text] [PDF]

				M. Montero, M. T. Alonso, A. Albillos, J. García-Sancho, and J. Alvarez Mitochondrial Ca2+-induced Ca2+ Release Mediated by the Ca2+ Uniporter Mol. Biol. Cell, January 1, 2001; 12(1): 63 - 71. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help