QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (25) Citing Articles via Google Scholar Google Scholar Articles by Liu, Q. Articles by Gardner, P. D. Search for Related Content PubMed PubMed Citation Articles by Liu, Q. Articles by Gardner, P. D.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 15, 1999, 19(22):9747-9755

Cell Type-Specific Activation of Neuronal Nicotinic Acetylcholine Receptor Subunit Genes by Sox10

Qun Liu, Irena N. Melnikova, Minjie Hu, and Paul D. Gardner

Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, Texas 78245-3207

The regulatory factor Sox10 is expressed in neural crest derivatives during development as well as in the adult CNS and peripheral nervous system. Mutations of the human Sox10 gene have been identified in patients with Waardenburg-Hirschsprung syndrome that is characterized by defects in neural crest development. Previous studies suggested that Sox10 might function as an important transcriptional regulator of neural crest development. No natural target genes of Sox10 have yet been identified. Although human Sox10 activates a synthetic promoter consisting of a TATA box and multiple Sox consensus sequences, no transcriptional activity of the rat Sox10 homolog has been detected. Here we report that the neuronal nicotinic acetylcholine receptor 4 and 3 subunit gene promoters are transactivated by rat Sox10 in a cell type-specific manner. The 3 and 4 subunits, in combination with the 5 subunit, make up the predominant nicotinic receptor subtype expressed in the peripheral nervous system. Transfections using Sox10 mutants indicate that the C-terminal region is dispensable for its ability to activate the 4 and 3 promoters. Rat Sox10 was originally identified as an accessory protein of the POU domain protein Tst-1/Oct6/SCIP in glial cells. Tst-1/Oct6/SCIP was shown previously to activate the 3 promoter. We now demonstrate that it can transactivate the 4 promoter as well. However, we were unable to detect any synergistic effects of Sox10 and Tst-1/Oct6/SCIP on 4 or 3 promoter activity. Finally, we present data suggesting that recombinant Sox10 protein can directly interact with a previously characterized regulatory region of the 4 gene.

Key words: Sox10; gene expression; nACh receptor; ligand-gated ion channel; transcriptional regulation; POU

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19229747-09$05.00/0

This article has been cited by other articles:

				Z. Mou, A. R. Tapper, and P. D. Gardner The Armadillo Repeat-containing Protein, ARMCX3, Physically and Functionally Interacts with the Developmental Regulatory Factor Sox10 J. Biol. Chem., May 15, 2009; 284(20): 13629 - 13640. [Abstract] [Full Text] [PDF]

				Y. F. F. Medel and P. D. Gardner Transcriptional Repression by a Conserved Intronic Sequence in the Nicotinic Receptor {alpha}3 Subunit Gene J. Biol. Chem., June 29, 2007; 282(26): 19062 - 19070. [Abstract] [Full Text] [PDF]

				R. Benfante, A. Flora, S. Di Lascio, F. Cargnin, R. Longhi, S. Colombo, F. Clementi, and D. Fornasari Transcription Factor PHOX2A Regulates the Human {alpha}3 Nicotinic Receptor Subunit Gene Promoter J. Biol. Chem., May 4, 2007; 282(18): 13290 - 13302. [Abstract] [Full Text] [PDF]

				X. Xu, M. M. Scott, and E. S. Deneris Shared Long-Range Regulatory Elements Coordinate Expression of a Gene Cluster Encoding Nicotinic Receptor Heteromeric Subtypes Mol. Cell. Biol., August 1, 2006; 26(15): 5636 - 5649. [Abstract] [Full Text] [PDF]

				L. M. Valor, M. Castillo, J. A. Ortiz, and M. Criado Transcriptional Regulation by Activation and Repression Elements Located at the 5'-Noncoding Region of the Human {alpha}9 Nicotinic Receptor Subunit Gene J. Biol. Chem., September 26, 2003; 278(39): 37249 - 37255. [Abstract] [Full Text] [PDF]

				M. Ilia, C. Beasley, D. Meijer, R. Kerwin, D. Cotter, I. Everall, and J. Price Expression of Oct-6, a POU III Domain Transcription Factor, in Schizophrenia Am J Psychiatry, July 1, 2002; 159(7): 1174 - 1182. [Abstract] [Full Text] [PDF]

				L. M. Valor, A. Campos-Caro, C. Carrasco-Serrano, J. A. Ortiz, J. J. Ballesta, and M. Criado Transcription Factors NF-Y and Sp1 Are Important Determinants of the Promoter Activity of the Bovine and Human Neuronal Nicotinic Receptor beta 4 Subunit Genes J. Biol. Chem., March 8, 2002; 277(11): 8866 - 8876. [Abstract] [Full Text] [PDF]

				K. A. Dutton, A. Pauliny, S. S. Lopes, S. Elworthy, T. J. Carney, J. Rauch, R. Geisler, P. Haffter, and R. N. Kelsh Zebrafish colourless encodes sox10 and specifies non-ectomesenchymal neural crest fates Development, November 1, 2001; 128(21): 4113 - 4125. [Abstract] [Full Text] [PDF]

				M H Sham, V C H Lui, M Fu, B Chen, and P K H Tam SOX10 is abnormally expressed in aganglionic bowel of Hirschsprung's disease infants Gut, August 1, 2001; 49(2): 220 - 226. [Abstract] [Full Text] [PDF]

				M. Lee, J. Goodall, C. Verastegui, R. Ballotti, and C. R. Goding Direct Regulation of the Microphthalmia Promoter by Sox10 Links Waardenburg-Shah Syndrome (WS4)-associated Hypopigmentation and Deafness to WS2 J. Biol. Chem., November 22, 2000; 275(48): 37978 - 37983. [Abstract] [Full Text] [PDF]

				I. N. Melnikova and P. D. Gardner The Signal Transduction Pathway Underlying Ion Channel Gene Regulation by Sp1-c-Jun Interactions J. Biol. Chem., May 25, 2001; 276(22): 19040 - 19045. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help