QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (29) Citing Articles via Google Scholar Google Scholar Articles by Enz, R. Articles by Cutting, G. R. Search for Related Content PubMed PubMed Citation Articles by Enz, R. Articles by Cutting, G. R.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 15, 1999, 19(22):9841-9847

Expression of the Voltage-Gated Chloride Channel ClC-2 in Rod Bipolar Cells of the Rat Retina

Ralf Enz², Brenda J. Ross¹, and Garry R. Cutting¹

¹ Institute of Genetic Medicine and Department of Pediatrics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, and ² Institut für Biochemie, Friedrich-Alexander-Universität Erlargen-Nürnberg, 91054 Erlangen, Germany

Voltage-gated chloride channels (ClC) are highly conserved during evolution and appear to participate in a variety of physiological functions. Recently, ClC-2 was proposed to play a role in stabilizing the chloride equilibrium potential near or below the resting membrane potential in neurons expressing ligand-gated chloride channels. Because rod bipolar cells in mammalian retina express three forms of inhibitory ligand-gated chloride channels, we decided to study ClC-2 localization and function in the rat retina. RNA encoding ClC-1, -2, -3, -4, and -5 was detected by reverse transcription-PCR in the rat retina. ClC-2-specific antibodies identified protein on cell bodies and in synaptic layers. Double-immunofluorescence staining revealed that intense ClC-2 immunoreactivity colocalized with PKC-stained rod bipolar cells. Patch-clamp experiments performed with individual rod bipolar cells demonstrated the presence of a time-dependent, inwardly rectified current activated at hyperpolarizing membrane potentials. This current demonstrated selectivity for different anions (Cl > I > gluconate), was inhibited by Cd²⁺, and was minimally reduced by 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid. These features are consistent with currents generated by ClC-2 channels. Our data indicate that functional ClC-2 channels are present in retinal rod bipolar cells and support a role for ClC-2 in maintaining Cl homeostasis in neurons with ligand-gated chloride channels.

Key words: voltage-gated chloride channel; ClC; ligand-gated ion channel; GABA receptor; CNS; retina; rod bipolar cell

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19229841-07$05.00/0

This article has been cited by other articles:

				I. F. Abdullaev, A. Rudkouskaya, G. P. Schools, H. K. Kimelberg, and A. A. Mongin Pharmacological comparison of swelling-activated excitatory amino acid release and Cl- currents in cultured rat astrocytes J. Physiol., May 1, 2006; 572(3): 677 - 689. [Abstract] [Full Text] [PDF]

				J. Cuppoletti, D. H. Malinowska, K. P. Tewari, Q.-j. Li, A. M. Sherry, M. L. Patchen, and R. Ueno SPI-0211 activates T84 cell chloride transport and recombinant human ClC-2 chloride currents Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1173 - C1183. [Abstract] [Full Text] [PDF]

				Y.-P. Ma, J. Cui, H.-J. Hu, and Z.-H. Pan Mammalian Retinal Bipolar Cells Express Inwardly Rectifying K+ Currents (IKir) With a Different Distribution Than That of Ih J Neurophysiol, November 1, 2003; 90(5): 3479 - 3489. [Abstract] [Full Text] [PDF]

				A. Gulacsi, C. R. Lee, A. Sik, T. Viitanen, K. Kaila, J. M. Tepper, and T. F. Freund Cell Type-Specific Differences in Chloride-Regulatory Mechanisms and GABAA Receptor-Mediated Inhibition in Rat Substantia Nigra J. Neurosci., September 10, 2003; 23(23): 8237 - 8246. [Abstract] [Full Text] [PDF]

				W. B. Thoreson, E. J. Bryson, and K. Rabl Reciprocal Interactions Between Calcium and Chloride in Rod Photoreceptors J Neurophysiol, September 1, 2003; 90(3): 1747 - 1753. [Abstract] [Full Text] [PDF]

				M. L. Olsen, S. Schade, S. A. Lyons, M. D. Amaral, and H. Sontheimer Expression of Voltage-Gated Chloride Channels in Human Glioma Cells J. Neurosci., July 2, 2003; 23(13): 5572 - 5582. [Abstract] [Full Text] [PDF]

				K. A. Parkerson and H. Sontheimer Contribution of chloride channels to volume regulation of cortical astrocytes Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1460 - C1467. [Abstract] [Full Text] [PDF]

				T. J. Jentsch, V. Stein, F. Weinreich, and A. A. Zdebik Molecular Structure and Physiological Function of Chloride Channels Physiol Rev, April 1, 2002; 82(2): 503 - 568. [Abstract] [Full Text] [PDF]

				K. Strange Of Mice and Worms: Novel Insights Into ClC-2 Anion Channel Physiology Physiology, February 1, 2002; 17(1): 11 - 16. [Abstract] [Full Text] [PDF]

				A. Wenning, C. F. J. Erxleben, and R. L. Calabrese Indirectly Gated Cl--Dependent Cl- Channels Sense Physiological Changes of Extracellular Chloride in the Leech J Neurophysiol, October 1, 2001; 86(4): 1826 - 1838. [Abstract] [Full Text] [PDF]

				N. K. Wills, T. Weng, L. Mo, H. L. Hellmich, A. Yu, T. Wang, S. Buchheit, and B. F. Godley Chloride Channel Expression in Cultured Human Fetal RPE Cells: Response to Oxidative Stress Invest. Ophthalmol. Vis. Sci., December 1, 2000; 41(13): 4247 - 4255. [Abstract] [Full Text]

				E. K. O'Donnell, R. L. Sedlacek, A. K. Singh, and B. D. Schultz Inhibition of enterotoxin-induced porcine colonic secretion by diarylsulfonylureas in vitro Am J Physiol Gastrointest Liver Physiol, November 1, 2000; 279(5): G1104 - G1112. [Abstract] [Full Text] [PDF]

				N. Vardi, L.-L. Zhang, J. A. Payne, and P. Sterling Evidence That Different Cation Chloride Cotransporters in Retinal Neurons Allow Opposite Responses to GABA J. Neurosci., October 15, 2000; 20(20): 7657 - 7663. [Abstract] [Full Text] [PDF]

				D. Duan, L. Ye, F. Britton, B. Horowitz, and J. R. Hume A Novel Anionic Inward Rectifier in Native Cardiac Myocytes Circ. Res., March 3, 2000; 86 (4): e63 - e71. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help