QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (30) Citing Articles via Google Scholar Google Scholar Articles by Wetzel, R. K. Articles by Sweadner, K. J. Search for Related Content PubMed PubMed Citation Articles by Wetzel, R. K. Articles by Sweadner, K. J.

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 15, 1999, 19(22):9878-9889

Cellular and Subcellular Specification of Na,K-ATPase  and  Isoforms in the Postnatal Development of Mouse Retina

Randall K. Wetzel, Elena Arystarkhova, and Kathleen J. Sweadner

Laboratory of Membrane Biology, Neuroscience Center, Massachusetts General Hospital, Charlestown, Massachusetts 02129

The Na,K-ATPase is a dominant factor in retinal energy metabolism, and unique combinations of isoforms of its  and  subunits are expressed in different cell types and determine its functional properties. We used isoform-specific antibodies and fluorescence confocal microscopy to determine the expression of Na,K-ATPase  and  subunits in the mouse and rat retina. In the adult retina, 1 was found in Müller and horizontal cells, 2 in some Müller glia, and 3 in photoreceptors and all retinal neurons. 1 was largely restricted to horizontal, amacrine, and ganglion cells; 2 was largely restricted to photoreceptors, bipolar cells, and Müller glia; and 3 was largely restricted to photoreceptors. Photoreceptor inner segments have the highest concentration of Na,K-ATPase in adult retinas. Isoform distribution exhibited marked changes during postnatal development. 3 and 2 were in undifferentiated photoreceptor somas at birth but only later were targeted to inner segments and synaptic terminals. 3, in contrast, was expressed late in photoreceptor differentiation and was immediately targeted to inner segments. A high level of 1 expression in horizontal cells preceded migration, whereas increases in 2 expression in bipolar cells occurred very late, coinciding with synaptogenesis in the inner plexiform layer. Most of the spatial specification of Na,K-ATPase isoform expression was completed before eye opening and the onset of electroretinographic responses on postnatal day 13 (P13), but quantitative increase continued until P22 in parallel with synaptogenesis.

Key words: Na,K-ATPase; retina; photoreceptor; development; isoforms; immunofluorescence

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/19229878-12$05.00/0

This article has been cited by other articles:

				A. Rajala, M. Tanito, Y. Z. Le, C. R. Kahn, and R. V. S. Rajala Loss of Neuroprotective Survival Signal in Mice Lacking Insulin Receptor Gene in Rod Photoreceptor Cells J. Biol. Chem., July 11, 2008; 283(28): 19781 - 19792. [Abstract] [Full Text] [PDF]

				M. C. M. Kwok, J. M. Holopainen, L. L. Molday, L. J. Foster, and R. S. Molday Proteomics of Photoreceptor Outer Segments Identifies a Subset of SNARE and Rab Proteins Implicated in Membrane Vesicle Trafficking and Fusion Mol. Cell. Proteomics, June 1, 2008; 7(6): 1053 - 1066. [Abstract] [Full Text] [PDF]

				L. L. Molday, W. W. H. Wu, and R. S. Molday Retinoschisin (RS1), the Protein Encoded by the X-linked Retinoschisis Gene, Is Anchored to the Surface of Retinal Photoreceptor and Bipolar Cells through Its Interactions with a Na/K ATPase-SARM1 Complex J. Biol. Chem., November 9, 2007; 282(45): 32792 - 32801. [Abstract] [Full Text] [PDF]

				B. A. Berkowitz, R. Roberts, H. Luan, D. Bissig, B. V. Bui, M. Gradianu, D. J. Calkins, and A. J. Vingrys Manganese-Enhanced MRI Studies of Alterations of Intraretinal Ion Demand in Models of Ocular Injury Invest. Ophthalmol. Vis. Sci., August 1, 2007; 48(8): 3796 - 3804. [Abstract] [Full Text] [PDF]

				J. A. Phipps, P. Yee, E. L. Fletcher, and A. J. Vingrys Rod photoreceptor dysfunction in diabetes: activation, deactivation, and dark adaptation. Invest. Ophthalmol. Vis. Sci., July 1, 2006; 47(7): 3187 - 3194. [Abstract] [Full Text] [PDF]

				O. Strauss The Retinal Pigment Epithelium in Visual Function Physiol Rev, July 1, 2005; 85(3): 845 - 881. [Abstract] [Full Text] [PDF]

				A. E. Moseley, S. P. Lieske, R. K. Wetzel, P. F. James, S. He, D. A. Shelly, R. J. Paul, G. P. Boivin, D. P. Witte, J. M. Ramirez, et al. The Na,K-ATPase alpha 2 Isoform Is Expressed in Neurons, and Its Absence Disrupts Neuronal Activity in Newborn Mice J. Biol. Chem., February 7, 2003; 278(7): 5317 - 5324. [Abstract] [Full Text] [PDF]

				D. Z. Ellis, J. Rabe, and K. J. Sweadner Global Loss of Na,K-ATPase and Its Nitric Oxide-Mediated Regulation in a Transgenic Mouse Model of Amyotrophic Lateral Sclerosis J. Neurosci., January 1, 2003; 23(1): 43 - 51. [Abstract] [Full Text] [PDF]

				R. V. S. Rajala, M. E. McClellan, J. D. Ash, and R. E. Anderson In Vivo Regulation of Phosphoinositide 3-Kinase in Retina through Light-induced Tyrosine Phosphorylation of the Insulin Receptor beta -Subunit J. Biol. Chem., November 1, 2002; 277(45): 43319 - 43326. [Abstract] [Full Text] [PDF]

				S. Pressmar, M. Ader, G. Richard, M. Schachner, and U. Bartsch The Fate of Heterotopically Grafted Neural Precursor Cells in the Normal and Dystrophic Adult Mouse Retina Invest. Ophthalmol. Vis. Sci., December 1, 2001; 42(13): 3311 - 3319. [Abstract] [Full Text] [PDF]

				R. K. Wetzel and K. J. Sweadner Immunocytochemical Localization of NaK-ATPase Isoforms in the Rat and Mouse Ocular Ciliary Epithelium Invest. Ophthalmol. Vis. Sci., March 1, 2001; 42(3): 763 - 769. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help