QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sanyal, S. Articles by Krishnan, K. S. Search for Related Content PubMed PubMed Citation Articles by Sanyal, S. Articles by Krishnan, K. S.

 Previous Article  |  Next Article 

The Journal of Neuroscience, 1999, 19:RC47:1-5

RAPID COMMUNICATION
Phenotypic Interaction between Temperature-Sensitive Paralytic Mutants comatose and paralytic Suggests a Role for N-Ethylmaleimide-Sensitive Fusion Factor in Synaptic Vesicle Cycling in Drosophila

Subhabrata Sanyal, Amit Basole, and K. S. Krishnan

Department of Biological Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai 400 005, India

The temperature-induced paralysis of comatose (comt) mutants of Drosophila is suggestive of a function for N-ethylmaleimide-sensitive fusion factor (NSF) in the CNS. Mutations in the para gene encoding the  subunit of the voltage-gated sodium channel also result in a similar phenotype. We show that paralysis in comt flies is activity-dependent, and in the doubly mutant comt para flies comt-like paralysis does not set in until the effects of para are reversed by shifting to permissive temperatures. During recording from the thoracic flight muscles, we observed that comt flies showed a burst of spontaneous activity at restrictive temperature. This has been reported earlier as a unique characteristic of comt paralysis. The comt para double mutant showed this burst of activity not at restrictive but only on shifting back to permissive temperature. The unusual behavior and electrophysiology of the doubly mutant flies reported here indicates a role for NSF in synaptic vesicle cycling.

Key words: synaptic vesicle cycling; NSF; paralytic mutants; comatose; para; flight muscle recording; Drosophila

---

Copyright © 0000 Society for Neuroscience  0270-6474/0/$05.00/0

This article has been cited by other articles:

				E. Grote, C. M. Carr, and P. J. Novick Ordering the Final Events in Yeast Exocytosis J. Cell Biol., October 16, 2000; 151(2): 439 - 452. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help