 |
 Previous Article | Next Article 
The Journal of Neuroscience, February 1, 1999, 19(3):1049-1061
Purification and Characterization of Astrocyte Precursor Cells in
the Developing Rat Optic Nerve
Huaiyu
Mi and
Ben A.
Barres
Stanford University School of Medicine, Department of Neurobiology,
Fairchild Science Building, Stanford, California 94305-5125
The signaling interactions that control oligodendrocyte generation
from their precursor cells have been studied intensively. Much less is
known about how astrocyte generation is normally controlled. Here we
report the purification and characterization of astrocyte precursor
cells (APCs) from the developing rat optic nerve. APCs are
antigenically distinct from astrocytes. Both cell types are
Pax2+ and vimentin+, whereas
astrocytes are GFAP+ and S100 +, and
the precursor cells are A2B5+. In contrast to
purified astrocytes, purified APCs rapidly die in serum-free culture
but can be saved by basic fibroblast growth factor (bFGF) and glial
growth factor 2 (GGF2). Unlike oligodendrocyte precursor cells,
APCs do not differentiate by default; their differentiation into
GFAP+ cells is induced by ciliary neurotrophic
factor (CNTF) or by leukemia inhibitory factor (LIF). Finally,
the survival, proliferation, and differentiation of APCs were promoted
by coculture with other embryonic optic nerve cell types but not with
purified embryonic retinal ganglion cells, indicating that interactions
with non-neuronal cells are likely to play an important role in
controlling astrocyte generation in the developing optic nerve.
Key words:
glial development; survival; proliferation; differentiation; glial growth factor (GGF); basic fibroblast growth
factor (bFGF); ciliary neurotrophic factor (CNTF); leukemia inhibitory
factor (LIF)
Copyright © 1999 Society for Neuroscience 0270-6474/99/1931049-13$05.00/0
This article has been cited by other articles:
|
|
|
|
 
A. J. Barker, S. M. Koch, J. Reed, B. A. Barres, and E. M. Ullian
Developmental Control of Synaptic Receptivity
J. Neurosci.,
August 13, 2008;
28(33):
8150 - 8160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Chatzopoulou, A. Miguez, M. Savvaki, G. Levasseur, A. Muzerelle, M.-P. Muriel, O. Goureau, K. Watanabe, L. Goutebroze, P. Gaspar, et al.
Structural Requirement of TAG-1 for Retinal Ganglion Cell Axons and Myelin in the Mouse Optic Nerve
J. Neurosci.,
July 23, 2008;
28(30):
7624 - 7636.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Herrmann, T. Imura, B. Song, J. Qi, Y. Ao, T. K. Nguyen, R. A. Korsak, K. Takeda, S. Akira, and M. V. Sofroniew
STAT3 is a Critical Regulator of Astrogliosis and Scar Formation after Spinal Cord Injury
J. Neurosci.,
July 9, 2008;
28(28):
7231 - 7243.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Mo, A. R. Moore, R. Filipovic, Y. Ogawa, I. Kazuhiro, S. D. Antic, and N. Zecevic
Human Cortical Neurons Originate from Radial Glia and Neuron-Restricted Progenitors
J. Neurosci.,
April 11, 2007;
27(15):
4132 - 4145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Tezel, X. Yang, C. Luo, Y. Peng, S. L. Sun, and D. Sun
Mechanisms of Immune System Activation in Glaucoma: Oxidative Stress-Stimulated Antigen Presentation by the Retina and Optic Nerve Head Glia
Invest. Ophthalmol. Vis. Sci.,
February 1, 2007;
48(2):
705 - 714.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhu, T. Harada, L. Liu, M. E. Lush, F. Guignard, C. Harada, D. K. Burns, M. L. Bajenaru, D. H. Gutmann, and L. F. Parada
Inactivation of NF1 in CNS causes increased glial progenitor proliferation and optic glioma formation
Development,
December 15, 2005;
132(24):
5577 - 5588.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. L. Evans and P. J. Gage
Expression of the homeobox gene Pitx2 in neural crest is required for optic stalk and ocular anterior segment development
Hum. Mol. Genet.,
November 15, 2005;
14(22):
3347 - 3359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Scheffler, N. M. Walton, D. D. Lin, A. K. Goetz, G. Enikolopov, S. N. Roper, and D. A. Steindler
Phenotypic and functional characterization of adult brain neuropoiesis
PNAS,
June 28, 2005;
102(26):
9353 - 9358.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. H. Mui, J. W. Kim, G. Lemke, and S. Bertuzzi
Vax genes ventralize the embryonic eye
Genes & Dev.,
May 15, 2005;
19(10):
1249 - 1259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. De Seranno, C. Estrella, A. Loyens, A. Cornea, S. R. Ojeda, J.-C. Beauvillain, and V. Prevot
Vascular Endothelial Cells Promote Acute Plasticity in Ependymoglial Cells of the Neuroendocrine Brain
J. Neurosci.,
November 17, 2004;
24(46):
10353 - 10363.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Miyawaki, A. Uemura, M. Dezawa, R. T. Yu, C. Ide, S. Nishikawa, Y. Honda, Y. Tanabe, and T. Tanabe
Tlx, an Orphan Nuclear Receptor, Regulates Cell Numbers and Astrocyte Development in the Developing Retina
J. Neurosci.,
September 15, 2004;
24(37):
8124 - 8134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Chan-Ling, D. S. McLeod, S. Hughes, L. Baxter, Y. Chu, T. Hasegawa, and G. A. Lutty
Astrocyte-Endothelial Cell Relationships during Human Retinal Vascular Development
Invest. Ophthalmol. Vis. Sci.,
June 1, 2004;
45(6):
2020 - 2032.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Yang, O. Agapova, A. Parker, W. Shannon, P. Pecen, J. Duncan, M. Salvador-Silva, and M. R. Hernandez
DNA microarray analysis of gene expression in human optic nerve head astrocytes in response to hydrostatic pressure
Physiol Genomics,
April 13, 2004;
17(2):
157 - 169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Okano-Uchida, T. Himi, Y. Komiya, and Y. Ishizaki
Cerebellar granule cell precursors can differentiate into astroglial cells
PNAS,
February 3, 2004;
101(5):
1211 - 1216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. R. Lo, L. L. Rowlette, M. Caballero, P. Yang, M. R. Hernandez, and T. Borras
Tissue Differential Microarray Analysis of Dexamethasone Induction Reveals Potential Mechanisms of Steroid Glaucoma
Invest. Ophthalmol. Vis. Sci.,
February 1, 2003;
44(2):
473 - 485.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Spassky, F. de Castro, B. Le Bras, K. Heydon, F. Queraud-LeSaux, E. Bloch-Gallego, A. Chedotal, B. Zalc, and J.-L. Thomas
Directional Guidance of Oligodendroglial Migration by Class 3 Semaphorins and Netrin-1
J. Neurosci.,
July 15, 2002;
22(14):
5992 - 6004.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Moon, J.-Y. Yoo, V. Matarazzo, Y. K. Sung, E. J. Kim, and G. V. Ronnett
Leukemia inhibitory factor inhibits neuronal terminal differentiation through STAT3 activation
PNAS,
June 25, 2002;
99(13):
9015 - 9020.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Goldberg, M. P. Klassen, Y. Hua, and B. A. Barres
Amacrine-Signaled Loss of Intrinsic Axon Growth Ability by Retinal Ganglion Cells
Science,
June 7, 2002;
296(5574):
1860 - 1864.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Gregori, C. Proschel, M. Noble, and M. Mayer-Proschel
The Tripotential Glial-Restricted Precursor (GRP) Cell and Glial Development in the Spinal Cord: Generation of Bipotential Oligodendrocyte-Type-2 Astrocyte Progenitor Cells and Dorsal-Ventral Differences in GRP Cell Function
J. Neurosci.,
January 1, 2002;
22(1):
248 - 256.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Morrow, M.-R. Song, and A. Ghosh
Sequential specification of neurons and glia by developmentally regulated extracellular factors
Development,
September 15, 2001;
128(18):
3585 - 3594.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Mi, H. Haeberle, and B. A. Barres
Induction of Astrocyte Differentiation by Endothelial Cells
J. Neurosci.,
March 1, 2001;
21(5):
1538 - 1547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Bertuzzi, R. Hindges, S. H. Mui, D. D.M. O'Leary, and G. Lemke
The homeodomain protein Vax1 is required for axon guidance and major tract formation in the developing forebrain
Genes & Dev.,
December 1, 1999;
13(23):
3092 - 3105.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. F. McManus, L.-C. Chen, I. Vallejo, and M. Vallejo
Astroglial Differentiation of Cortical Precursor Cells Triggered by Activation of the cAMP-Dependent Signaling Pathway
J. Neurosci.,
October 15, 1999;
19(20):
9004 - 9015.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Wallace and M. Raff
A role for Sonic hedgehog in axon-to-astrocyte signalling in the rodent optic nerve
Development,
January 7, 1999;
126(13):
2901 - 2909.
[Abstract]
[PDF]
|
|
|
|
|
