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The Journal of Neuroscience, March 1, 1999, 19(5):1663-1674
Ionic Currents Underlying Spontaneous Action Potentials in Isolated Cerebellar Purkinje Neurons
Indira M. Raman and Bruce P. Bean Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115
Acutely dissociated cell bodies of mouse Purkinje neurons spontaneously fired action potentials at ~50 Hz (25°C). To directly measure the ionic currents underlying spontaneous activity, we voltage-clamped the cells using prerecorded spontaneous action potentials (spike trains) as voltage commands and used ionic substitution and selective blockers to isolate individual currents. The largest current flowing during the interspike interval was tetrodotoxin-sensitive sodium current (approximately
50 pA between
Key words: cerebellum; sodium current; calcium current; potassium current; Ih; spike; pacemaking; pacemaker
Copyright © 1999 Society for Neuroscience 0270-6474/99/1951663-12$05.00/0
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