WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (56)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zheng, J. L.
Right arrow Articles by Gao, W.-Q.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zheng, J. L.
Right arrow Articles by Gao, W.-Q.

 Previous Article  |  Next Article 

The Journal of Neuroscience, March 15, 1999, 19(6):2161-2170

Immunocytochemical and Morphological Evidence for Intracellular Self-Repair as an Important Contributor to Mammalian Hair Cell Recovery

J. Lisa Zheng1, Gilbert Keller2, and Wei-Qiang Gao1

Departments of 1 Neuroscience and 2 Pharmacokinetics and Metabolism, Genentech, South San Francisco, California 94080

Although recent studies have provided evidence for hair cell regeneration in mammalian inner ears, the mechanism underlying this regenerative process is still under debate. Here we report immunocytochemical, histological, electron microscopic, and autoradiographic evidence that, in cultured postnatal rat utricles, a substantial number of hair cells can survive gentamicin insult even their stereocilia are lost. These partially damaged hair cells can survive for a prolonged time and regrow the stereocilia. Although the number of stereocilia-bearing hair cells increases over time after gentamicin insult, hair cell and supporting cell numbers remain essentially unchanged. Tritiated thymidine autoradiography and bromodeoxyuridine immunocytochemistry of the cultures demonstrate that cell proliferation in the sensory epithelium is very limited and is far below the number of recovered hair cells. Furthermore, terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling analysis indicates that gentamicin-induced apoptosis in the sensory epithelium occurs mainly during a 2 d treatment period, and additional cell death is minimal 2-11 d after treatment. Considered together, intracellular repair of partially damaged hair cells can be an important contributor to spontaneous hair cell recovery in mammalian inner ears.

Key words: hair cells; supporting cells; regeneration; self-repair; apoptosis; proliferation; vestibular; utricle; inner ear; gentamicin; autoradiography

Copyright © 1999 Society for Neuroscience  0270-6474/99/1962161-10$05.00/0


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Wei, S. Levic, L. Nie, W.-q. Gao, C. Petit, E. G. Jones, and E. N. Yamoah
Cells of adult brain germinal zone have properties akin to hair cells and can be used to replace inner ear sensory cells after damage
PNAS, December 30, 2008; 105(52): 21000 - 21005.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Hu and J. T. Corwin
Inner ear hair cells produced in vitro by a mesenchymal-to-epithelial transition
PNAS, October 16, 2007; 104(42): 16675 - 16680.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
C. Soler-Martin, N. Diez-Padrisa, P. Boadas-Vaello, and J. Llorens
Behavioral Disturbances and Hair Cell Loss in the Inner Ear Following Nitrile Exposure in Mice, Guinea Pigs, and Frogs
Toxicol. Sci., March 1, 2007; 96(1): 123 - 132.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
T. P. Hirvonen, L. B. Minor, T. E. Hullar, and J. P. Carey
Effects of Intratympanic Gentamicin on Vestibular Afferents and Hair Cells in the Chinchilla
J Neurophysiol, February 1, 2005; 93(2): 643 - 655.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. I. Matsui, J. M. Ogilvie, and M. E. Warchol
Inhibition of Caspases Prevents Ototoxic and Ongoing Hair Cell Death
J. Neurosci., February 15, 2002; 22(4): 1218 - 1227.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. D. Kopke, R. L. Jackson, G. Li, M. D. Rasmussen, M. E. Hoffer, D. A. Frenz, M. Costello, P. Schultheiss, and T. R. Van De Water
Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function
PNAS, April 25, 2001; (2001) 101120898.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. S. Stone and E. W Rubel
Cellular studies of auditory hair cell regeneration in birds
PNAS, October 24, 2000; 97(22): 11714 - 11721.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. A. Baird, M. D. Burton, D. S. Fashena, and R. A. Naeger
Hair cell recovery in mitotically blocked cultures of the bullfrog saccule
PNAS, October 24, 2000; 97(22): 11722 - 11729.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Zheng, J Shou, F Guillemot, R Kageyama, and W. Gao
Hes1 is a negative regulator of inner ear hair cell differentiation
Development, January 11, 2000; 127(21): 4551 - 4560.
[Abstract] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. D. Kopke, R. L. Jackson, G. Li, M. D. Rasmussen, M. E. Hoffer, D. A. Frenz, M. Costello, P. Schultheiss, and T. R. Van de Water
Growth factor treatment enhances vestibular hair cell renewal and results in improved vestibular function
PNAS, May 8, 2001; 98(10): 5886 - 5891.
[Abstract] [Full Text] [PDF]


-
-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-