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The Journal of Neuroscience, April 1, 1999, 19(7):2511-2521

Synaptic Vesicle Dynamics in Rat Fast and Slow Motor Nerve Terminals

Brian Reid¹, Clarke R. Slater², and Guy S. Bewick¹

¹ Department of Biomedical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen AB25 2ZD, United Kingdom, and ² Department of Neurobiology, The Medical School, University of Newcastle, Newcastle upon Tyne NE2 4HH, United Kingdom

We have investigated whether rat motor nerve terminals with different in vivo activity patterns also have different vesicle trafficking characteristics. To do this, we monitored, using combined optical and electrical techniques, the rate of exocytosis (during different frequencies and patterns of activity), the releasable pool size, and the recycle time of synaptic vesicles in terminals on soleus (slow-twitch) and extensor digitorum longus [(EDL); fast-twitch] muscle fibers. EDL terminals had a higher initial quantal content (QC) than soleus, but during tonic or phasic stimulation at 20-80 Hz, EDL QC ran down to a greater extent than soleus QC. By recording loss of fluorescence from exocytosing vesicles labeled with the dye FM1-43, EDL terminals were found to destain faster than those in soleus. Simultaneous intracellular recording of end plate potentials, to count the number of vesicles released, permitted estimation of the total vesicle pool (VP) size and the recycle time by combining the optical and electrophysiological data. Soleus vesicle pool was larger than EDL, but recycle time was not significantly different. These terminals, therefore, are adapted to their in vivo activity patterns by alterations in QC and VP size but not recycle time.

Key words: synaptic vesicles; vesicle recycling; FM1-43; exocytosis; quantal content; neuromuscular junction

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Copyright © 1999 Society for Neuroscience  0270-6474/99/1972511-11$05.00/0

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