 |
Previous Article | Next Article 
The Journal of Neuroscience, April 15, 1999, 19(8):2938-2944
The Metabotropic Receptor mGluR6 May Signal Through
Go, But Not Phosphodiesterase, in Retinal Bipolar Cells
Scott
Nawy
Departments of Ophthalmology and Visual Science, and Neuroscience,
Albert Einstein College of Medicine, Bronx, New York 10461
Bipolar cells are retinal interneurons that receive synaptic input
from photoreceptors. Glutamate, the photoreceptor transmitter, hyperpolarizes On bipolar cells by closing nonselective cation channels, an effect mediated by the metabotropic receptor mGluR6. Previous studies of mGluR6 transduction have suggested that the receptor couples to a phosphodiesterase (PDE) that preferentially hydrolyzes cGMP, and that cGMP directly gates the nonselective cation
channel. This hypothesis was tested by dialyzing On bipolar cells with
nonhydrolyzable analogs of cGMP. Whole-cell recordings were obtained
from On bipolar cells in slices of larval tiger salamander retina.
Surprisingly, On bipolar cells dialyzed with 8-(4-chlorophenylthio)-cyclic GMP (8-pCPT-cGMP), or 8-bromo-cyclic GMP
(8-Br-cGMP) responded normally to glutamate or
L-2-amino-4-phosphonobutyrate (L-APB). Response
amplitudes and kinetics were not significantly altered compared
with cells dialyzed with cGMP alone. Comparable results were obtained
with the PDE inhibitor 3-isobutyl-1-methyl-xanthine (IBMX) or with
8-pCPT-cGMP and IBMX together, indicating that PDE is not required for
mGluR6 signal transduction. Addition of the G-protein subunit
Go to the pipette solution suppressed the cation current
and occluded the glutamate response, whereas dialysis with
Gi or with transducin G had no significant effect
on either the cation current or the response. Dialysis of an antibody
directed against Go also reduced the glutamate response,
indicating a functional role for endogenous Go . These
results indicate that mGluR6 may signal through Go,
rather than a transducin-like G-protein.
Key words:
cGMP; 8-pCPT-cGMP; mGluR6; phosphodiesterase; retinal
bipolar cell; Go; phosphodiesterase
Copyright © 1999 Society for Neuroscience 0270-6474/99/1982938-07$05.00/0
This article has been cited by other articles:

|
 |

|
 |
 
Y. Ping, H. Huang, X.-J. Zhang, and X.-L. Yang
Melatonin potentiates rod signals to ON type bipolar cells in fish retina
J. Physiol.,
June 1, 2008;
586(11):
2683 - 2694.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Rao, R. Dallman, S. Henderson, and C.-K. Chen
G 5 Is Required for Normal Light Responses and Morphology of Retinal ON-Bipolar Cells
J. Neurosci.,
December 19, 2007;
27(51):
14199 - 14204.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Schein and K. M. Ahmad
Efficiency of Synaptic Transmission of Single-Photon Events from Rod Photoreceptor to Rod Bipolar Dendrite
Biophys. J.,
November 1, 2006;
91(9):
3257 - 3267.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Xia, R. C. Carroll, and S. Nawy
State-dependent AMPA receptor trafficking in the Mammalian retina.
J. Neurosci.,
May 10, 2006;
26(19):
5028 - 5036.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y.-C. Yu, L.-H. Cao, and X.-L. Yang
Modulation by Brain Natriuretic Peptide of GABA Receptors on Rat Retinal ON-Type Bipolar Cells
J. Neurosci.,
January 11, 2006;
26(2):
696 - 707.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
N. Hosoi, I. Arai, and M. Tachibana
Group III Metabotropic Glutamate Receptors and Exocytosed Protons Inhibit L-Type Calcium Currents in Cones But Not in Rods
J. Neurosci.,
April 20, 2005;
25(16):
4062 - 4072.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Snellman and S. Nawy
cGMP-Dependent Kinase Regulates Response Sensitivity of the Mouse On Bipolar Cell
J. Neurosci.,
July 21, 2004;
24(29):
6621 - 6628.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Nawy
Desensitization of the mGluR6 transduction current in tiger salamander On bipolar cells
J. Physiol.,
July 1, 2004;
558(1):
137 - 146.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Dhingra, E. Faurobert, N. Dascal, P. Sterling, and N. Vardi
A Retinal-Specific Regulator of G-Protein Signaling Interacts with G{alpha}o and Accelerates an Expressed Metabotropic Glutamate Receptor 6 Cascade
J. Neurosci.,
June 23, 2004;
24(25):
5684 - 5693.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
D. Henry, S. Burke, E. Shishido, and G. Matthews
Retinal Bipolar Neurons Express the Cyclic Nucleotide-Gated Channel of Cone Photoreceptors
J Neurophysiol,
February 1, 2003;
89(2):
754 - 761.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. G. Gregg, S. Mukhopadhyay, S. I. Candille, S. L. Ball, M. T. Pardue, M. A. McCall, and N. S. Peachey
Identification of the Gene and the Mutation Responsible for the Mouse nob Phenotype
Invest. Ophthalmol. Vis. Sci.,
January 1, 2003;
44(1):
378 - 384.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
J. Snellman and S. Nawy
Regulation of the Retinal Bipolar Cell mGluR6 Pathway by Calcineurin
J Neurophysiol,
September 1, 2002;
88(3):
1088 - 1096.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
U. B. Kaupp and R. Seifert
Cyclic Nucleotide-Gated Ion Channels
Physiol Rev,
July 1, 2002;
82(3):
769 - 824.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Dhingra, M. Jiang, T.-L. Wang, A. Lyubarsky, A. Savchenko, T. Bar-Yehuda, P. Sterling, L. Birnbaumer, and N. Vardi
Light Response of Retinal ON Bipolar Cells Requires a Specific Splice Variant of Galpha o
J. Neurosci.,
June 15, 2002;
22(12):
4878 - 4884.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
F. Rieke
Temporal Contrast Adaptation in Salamander Bipolar Cells
J. Neurosci.,
December 1, 2001;
21(23):
9445 - 9454.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
H. Yamada and K. Nakatani
Odorant-induced Hyperpolarization and Suppression of cAMP-activated Current in Newt Olfactory Receptor Neurons
Chem Senses,
January 1, 2001;
26(1):
25 - 34.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
A. Dhingra, A. Lyubarsky, M. Jiang, E. N. Pugh Jr, L. Birnbaumer, P. Sterling, and N. Vardi
The Light Response of ON Bipolar Neurons Requires G{alpha}o
J. Neurosci.,
December 15, 2000;
20(24):
9053 - 9058.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
M. d. P. Gomez and E. Nasi
Light Transduction in Invertebrate Hyperpolarizing Photoreceptors: Possible Involvement of a Go-Regulated Guanylate Cyclase
J. Neurosci.,
July 15, 2000;
20(14):
5254 - 5263.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Nawy
Regulation of the On Bipolar Cell mGluR6 Pathway by Ca2+
J. Neurosci.,
June 15, 2000;
20(12):
4471 - 4479.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|