 |
 Previous Article | Next Article 
The Journal of Neuroscience, April 15, 1999, 19(8):2954-2959
AMPA Receptor Activates a G-Protein that Suppresses a
cGMP-Gated Current
Fusao
Kawai1, 2 and
Peter
Sterling1
1 Department of Neuroscience, University of
Pennsylvania, Philadelphia, Pennsylvania 19104-6058, and
2 Department of Information Physiology, National Institute
for Physiological Sciences, Okazaki 444, Japan
The AMPA receptor, ubiquitous in brain, is termed "ionotropic"
because it gates an ion channel directly. We found that an AMPA
receptor can also modulate a G-protein to gate an ion channel indirectly. Glutamate applied to a retinal ganglion cell briefly suppresses the inward current through a cGMP-gated channel. AMPA and
kainate also suppress the current, an effect that is blocked both by
their general antagonist CNQX and also by the relatively specific AMPA
receptor antagonist GYKI-52466. Neither NMDA nor agonists of
metabotropic glutamate receptors are effective. The AMPA-induced
suppression of the cGMP-gated current is blocked when the patch pipette
includes GDP- -S, whereas the suppression is irreversible when the
pipette contains GTP- -S. This suggests a G-protein mediator, and,
consistent with this, pertussis toxin blocks the current suppression.
Nitric oxide (NO) donors induce the current suppressed by AMPA, and
phosphodiesterase inhibitors prevent the suppression. Apparently, the
AMPA receptor can exhibit a "metabotropic" activity that
allows it to antagonize excitation evoked by NO.
Key words:
AMPA; glutamate; ionotropic; metabotropic; G-protein; retina; rat
Copyright © 1999 Society for Neuroscience 0270-6474/99/1982954-06$05.00/0
This article has been cited by other articles:
|
|
|
|
 
J. V. Negrete-Diaz, T. S. Sihra, J. M. Delgado-Garcia, and A. Rodriguez-Moreno
Kainate Receptor-Mediated Inhibition of Glutamate Release Involves Protein Kinase A in the Mouse Hippocampus
J Neurophysiol,
October 1, 2006;
96(4):
1829 - 1837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J. Liu, A. Kalous, E. L. Werry, and M. R. Bennett
Purine Release from Spinal Cord Microglia after Elevation of Calcium by Glutamate
Mol. Pharmacol.,
September 1, 2006;
70(3):
851 - 859.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Takago, Y. Nakamura, and T. Takahashi
G protein-dependent presynaptic inhibition mediated by AMPA receptors at the calyx of Held
PNAS,
May 17, 2005;
102(20):
7368 - 7373.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Huettner
Spine-Tingling Excitement from Glutamate Receptors
Sci. Signal.,
November 25, 2003;
2003(210):
pe53 - pe53.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Peineau, B. Potier, F. Petit, P. Dournaud, J. Epelbaum, and R. Gardette
AMPA-sst2 somatostatin receptor interaction in rat hypothalamus requires activation of NMDA and/or metabotropic glutamate receptors and depends on intracellular calcium
J. Physiol.,
January 1, 2003;
546(1):
101 - 117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. B. Kaupp and R. Seifert
Cyclic Nucleotide-Gated Ion Channels
Physiol Rev,
July 1, 2002;
82(3):
769 - 824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L Linn and A. C Gafka
Modulation of a voltage-gated calcium channel linked to activation of glutamate receptors and calcium-induced calcium release in the catfish retina
J. Physiol.,
August 15, 2001;
535(1):
47 - 63.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Lerma, A. V. Paternain, A. Rodriguez-Moreno, and J. C. Lopez-Garcia
Molecular Physiology of Kainate Receptors
Physiol Rev,
July 1, 2001;
81(3):
971 - 998.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Frerking, D. Schmitz, Q. Zhou, J. Johansen, and R. A. Nicoll
Kainate Receptors Depress Excitatory Synaptic Transmission at CA3{right-arrow}CA1 Synapses in the Hippocampus via a Direct Presynaptic Action
J. Neurosci.,
May 1, 2001;
21(9):
2958 - 2966.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. F. M. Prinsen, R. T. Szerencsei, and P. P. M. Schnetkamp
Molecular Cloning and Functional Expression of the Potassium-Dependent Sodium-Calcium Exchanger from Human and Chicken Retinal Cone Photoreceptors
J. Neurosci.,
February 15, 2000;
20(4):
1424 - 1434.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. B. Cook, P. D. Lukasiewicz, and J. S. McReynolds
GABAC Receptors Control Adaptive Changes in a Glycinergic Inhibitory Pathway in Salamander Retina
J. Neurosci.,
January 15, 2000;
20(2):
806 - 812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
