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The Journal of Neuroscience, April 15, 1999, 19(8):3023-3032
Glutamate Receptor Expression Regulates Quantal Size and Quantal Content at the Drosophila Neuromuscular Junction
Aaron DiAntonio1, Sophie A. Petersen1, Manfred Heckmann2, and Corey S. Goodman1 1 Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, and 2 Physiologisches Institut der Technischen Universität München, 80802 Munich, Germany
At the Drosophila glutamatergic neuromuscular junction, the postsynaptic cell can regulate synaptic strength by both changing its sensitivity to neurotransmitter and generating a retrograde signal that regulates presynaptic transmitter release. To investigate the molecular mechanisms underlying these forms of plasticity, we have undertaken a genetic analysis of two postsynaptic glutamate receptors that are expressed at this synapse. Deletion of both genes results in embryonic lethality that can be rescued by transgenic expression of either receptor. Although these receptors are redundant for viability, they have important differences. By transgenically rescuing the double mutant, we have investigated the relationship of receptor gene dosage and composition to synaptic function. We find that the receptor subunit composition regulates quantal size, Argiotoxin sensitivity, and receptor desensitization kinetics. Finally, we show that the activity of the receptor can regulate the retrograde signal functioning at this synapse. Thus, the diversity of receptors expressed at this synapse provides the cell with mechanisms for generating synaptic plasticity.
Key words: glutamate receptor; Drosophila; neuromuscular junction; retrograde signal; genetics; synaptic plasticity; quantal size; quantal content
Copyright © 1999 Society for Neuroscience 0270-6474/99/1983023-10$05.00/0
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