QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (45) Citing Articles via Google Scholar Google Scholar Articles by Yu, X. H. Articles by De Koninck, Y. Search for Related Content PubMed PubMed Citation Articles by Yu, X. H. Articles by De Koninck, Y. Pubmed/NCBI databases Substance via MeSH

 Previous Article  |  Next Article 

The Journal of Neuroscience, May 1, 1999, 19(9):3545-3555

NK-1 Receptor Immunoreactivity in Distinct Morphological Types of Lamina I Neurons of the Primate Spinal Cord

X. H. Yu¹, E.-T. Zhang³, A. D. Craig³, R. Shigemoto⁴, A. Ribeiro-da-Silva^{1, 2}, and Y. De Koninck¹

¹ Department of Pharmacology and Therapeutics, and ² Anatomy and Cell Biology, McGill University, Montréal, Quebec, H3G 1Y6 Canada, ³ Division of Neurobiology, Barrow Neurological Institute, Phoenix, Arizona 85013, and ⁴ National Institute for Physiological Sciences, Okazaki 444-8585, Japan

In cat and monkey, lamina I cells can be classified into three basic morphological types (fusiform, pyramidal, and multipolar), and recent intracellular labeling evidence in the cat indicates that fusiform and multipolar lamina I cells are two different types of nociceptive cells, whereas pyramidal cells are innocuous thermoreceptive-specific. Because earlier observations indicated that only nociceptive dorsal horn neurons respond to substance P (SP), we examined which morphological types of lamina I neurons express receptors for SP (NK-1r).

We categorized NK-1r-immunoreactive (IR) lamina I neurons in serial horizontal sections from the cervical and lumbar enlargements of four monkeys. Consistent results were obtained by two independent teams of observers. Nearly all NK-1r-IR cells were fusiform (42%) or multipolar (43%), but only 6% were pyramidal (with 9% unclassified). We obtained similar findings in three monkeys in which we used double-labeling immunocytochemistry to identify NK-1r-IR and spinothalamic lamina I neurons retrogradely labeled with cholera toxin subunit b from the thalamus; most NK-1r-IR lamina I spinothalamic neurons were fusiform (48%) or multipolar (33%), and only 10% were pyramidal. In contrast, most (~75%) pyramidal and some (~25%) fusiform and multipolar lamina I spinothalamic neurons did not display NK-1r immunoreactivity.

These data indicate that most fusiform and multipolar lamina I neurons in the monkey can express NK-1r, consistent with the idea that both types are nociceptive, whereas only a small proportion of lamina I pyramidal cells express this receptor, consistent with the previous finding that they are non-nociceptive. However, these findings also indicate that not all nociceptive lamina I neurons express receptors for SP.

Key words: dorsal horn; spinothalamic; nociception; substance P; monkey; functional specialization

---

Copyright © 1999 Society for Neuroscience  0270-6474/99/1993545-11$05.00/0

This article has been cited by other articles:

				B. K. Rycroft, K. S. Vikman, and M. J. Christie Inflammation reduces the contribution of N-type calcium channels to primary afferent synaptic transmission onto NK1 receptor-positive lamina I neurons in the rat dorsal horn J. Physiol., May 1, 2007; 580(3): 883 - 894. [Abstract] [Full Text] [PDF]

				C. Patte-Mensah, C. Kibaly, and A. G. Mensah-Nyagan Substance P inhibits progesterone conversion to neuroactive metabolites in spinal sensory circuit: A potential component of nociception PNAS, June 21, 2005; 102(25): 9044 - 9049. [Abstract] [Full Text] [PDF]

				G. J. McCleane, R. Suzuki, and A. H. Dickenson Does a Single Intravenous Injection of the 5HT3 Receptor Antagonist Ondansetron Have an Analgesic Effect in Neuropathic Pain? A Double-Blinded, Placebo-Controlled Cross-Over Study Anesth. Analg., November 1, 2003; 97(5): 1474 - 1478. [Abstract] [Full Text] [PDF]

				A. J. Todd, Z. Puskar, R. C. Spike, C. Hughes, C. Watt, and L. Forrest Projection Neurons in Lamina I of Rat Spinal Cord with the Neurokinin 1 Receptor Are Selectively Innervated by Substance P-Containing Afferents and Respond to Noxious Stimulation J. Neurosci., May 15, 2002; 22(10): 4103 - 4113. [Abstract] [Full Text] [PDF]

				S. A Prescott and Y. D. Koninck Four cell types with distinctive membrane properties and morphologies in lamina I of the spinal dorsal horn of the adult rat J. Physiol., March 15, 2002; 539(3): 817 - 836. [Abstract] [Full Text] [PDF]

				A. D. Craig, K. Krout, and D. Andrew Quantitative Response Characteristics of Thermoreceptive and Nociceptive Lamina I Spinothalamic Neurons in the Cat J Neurophysiol, September 1, 2001; 86(3): 1459 - 1480. [Abstract] [Full Text] [PDF]

				A. D. Craig and J. O. Dostrovsky Differential Projections of Thermoreceptive and Nociceptive Lamina I Trigeminothalamic and Spinothalamic Neurons in the Cat J Neurophysiol, August 1, 2001; 86(2): 856 - 870. [Abstract] [Full Text] [PDF]

				A. Blomqvist, E.-T. Zhang, and A. D. Craig Cytoarchitectonic and immunohistochemical characterization of a specific pain and temperature relay, the posterior portion of the ventral medial nucleus, in the human thalamus Brain, March 1, 2000; 123(3): 601 - 619. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help