WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Stereo Investigator
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Freschi, J. E.
Right arrow Articles by Shain, W. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Freschi, J. E.
Right arrow Articles by Shain, W. G.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 2, 106-112, Copyright © 1982 by Society for Neuroscience

ARTICLE

Electrophysiological and pharmacological characteristics of the serotonin response on a vertebrate neuronal somatic cell hybrid

JE Freschi and WG Shain

We studied the effects of 5-hydroxytryptamine (5-HT, serotonin) on the vertebrate neuronal somatic cell hybrid TCX11. We compared electrophysiological and pharmacological properties of the 5-HT response to those of the dopamine (DA) response. The cells developed delayed rectification and the ability to generate action potentials. Both 5-HT and DA caused graded depolarizations associated with increases in membrane conductance. The cells, however, were 10 to 100 times more sensitive to 5-HT than to DA. Responses to the amines desensitized during sequential or continuous application, and each neurotransmitter could desensitize the cell to a subsequent dose of the other neurotransmitter (cross-desensitization). Reversal potentials for both amines ranged from 0 to 15 mV from cell to cell, but on any given cell, the reversal potentials for 5-HT and DA were equal. We applied a variety of drugs, including classical DA and 5-HT antagonists, and found no drug that blocked the response to one amine without a similar inhibition of the response to the other amine. We conclude that, on the hybrid TCX11, there is a receptor that mediates a depolarizing response with a conductance increase after interaction with 5-HT and DA. Our data suggest that the receptor is best classified as a serotonin receptor.




-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-