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Journal of Neuroscience, Vol 2, 889-894, Copyright © 1982 by Society for Neuroscience
Single channel currents activated by gamma-aminobutyric acid, muscimol, and (-)-pentobarbital in cultured mouse spinal neurons
MB Jackson, H Lecar, DA Mathers and JL Barker
The patch electrode technique was used to record single channel current
pulses in tissue-cultured mouse spinal cord neurons. In agreement with
earlier noise studies, channels activated by gamma-aminobutyric acid
(GABA), muscimol, and (-)-pentobarbital were found to have equal unit
conductances. The kinetics of channel closing were studied by analyzing the
distributions of open state lifetimes. Channels activated by (-)-
pentobarbital and muscimol had longer mean open times than channels
activated by GABA. As a result, the kinetics of (-)-pentobarbital- and
muscimol-activated channels could be studied in greater detail. Most
observed open state lifetime distributions were not exponential but
contained an excess of short duration events. A sum of two exponential
functions gave a much better fit than a single exponential function to most
observed open state lifetime distributions. A critical comparison of noise
analysis with single channel recording shows that the fast process
responsible for the rapid closures would be very difficult to detect in a
noise experiment. The channel noise is dominated by the slower process, and
as a result, the relaxation time of the slower kinetic component derived
from single channel studies is close to the mean open state lifetime
derived from noise measurements. The observation of a faster process points
toward either an additional population of channels or a scheme for the
channel closing transition which is not a simple first order process.
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