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Journal of Neuroscience, Vol 2, 1242-1251, Copyright © 1982 by Society for Neuroscience

ARTICLE

Neuropeptide proctolin associated with an identified skeletal motoneuron

M O'Shea and CA Bishop

Our objective was to find large and identifiable peptide-containing neurons and their postsynaptic targets. For this, a whole mount immunohistochemical method was used to locate cell bodies of neurons in the cockroach central nervous system immunoreactive to antibodies raised against the pentapeptide proctolin (H-Arg-Tyr-Leu-Pro-Thr-OH). The morphology and projections of the immunoreactive neurons were investigated by combining intracellular dye injection and electrophysiological and immunochemical procedures. The presence of proctolin in specific immunoreactive neurons was check by high pressure liquid chromatography combined with bioassay applied to extracts made from individually identified and isolated cell bodies. Using these approaches, we have identified a large proctolin-containing motoneuron which innervates muscles in a proximal segment of the cockroach leg. This study establishes a well characterized cellular preparation in which the physiological actions and functions of a peptide-containing neuron can be studied in vivo.


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M. Adams and M O'Shea
Peptide cotransmitter at a neuromuscular junction
Science, July 15, 1983; 221(4607): 286 - 289.
[Abstract] [PDF]


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Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
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