 |
 Previous Article | Next Article 
The Journal of Neuroscience, January 1, 2000, 20(1):251-258
AMPA-Kainate Subtypes of Glutamate Receptor in Rat Cerebral
Microglia
Mami
Noda1,
Hiroshi
Nakanishi2,
Junichi
Nabekura1, and
Norio
Akaike1
1 Laboratory of Cellular and System Physiology,
Graduate School of Medical Science, and 2 Department of
Pharmacology, Faculty of Dentistry, Kyushu University, Fukuoka
812-8582, Japan
Microglial cells were isolated from rat cerebral cortex, and
kainate (KA)-induced inward current was measured at a holding potential
of  40 or 60 mV. 6-Cyano-7-nitroquinoxaline-2,
3-dione-sensitive KA-induced currents increased with increasing
KA concentration. The half-activation concentration and Hill
coefficient were 3.3 × 10 4 M and
1.4, respectively. Although glutamate (Glu) and AMPA-induced currents were much smaller than that induced by KA, all KA-, Glu-, and
AMPA-induced currents were greatly and consistently enhanced in the
presence of cyclothiazide (CTZ). On the other hand, KA-induced currents
were much less sensitive to potentiation by concanavain A, suggesting
that the KA-induced response in rat microglia is predominantly mediated
by AMPA-preferring receptors (subunits GluR1-GluR4). The
current-voltage relationships of KA- and AMPA-CTZ-induced currents
were almost linear or slightly outward rectifying. The reversal
potential of KA-induced current shifted to negative potentials (from +4
to 40 mV) on switching from high Na+ to high
Ca2+ external solution, indicating the low
Ca2+ permeability through the AMPA-KA receptor
channel complexes. AMPA-KA receptor expression was studied with
immunohistochemistry and reverse transcription-PCR, from which GluR2,
GluR3, GluR4, and GluR5 were identified. Lower levels of mRNAs for
GluR7 and KA-1-KA-2 were also indicated. Finally, activation of
these receptors with KA or Glu significantly enhanced the production of
tumor necrosis factor- . These results suggest that primary cultured rat microglia possesses functional Glu receptor, which may mediate neuron to microglia communication in the physiological and pathological states.
Key words:
microglia; whole-cell patch clamp; kainate; glutamate; AMPA; cyclothiazide; reverse transcription-PCR; tumor necrosis
factor-
Copyright © 2000 Society for Neuroscience 0270-6474/0/201251-08$05.00/0
This article has been cited by other articles:
|
|
|
|
 
H. Wake, A. J. Moorhouse, S. Jinno, S. Kohsaka, and J. Nabekura
Resting Microglia Directly Monitor the Functional State of Synapses In Vivo and Determine the Fate of Ischemic Terminals
J. Neurosci.,
April 1, 2009;
29(13):
3974 - 3980.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I.-H. Cho, J. Hong, E. C. Suh, J. H. Kim, H. Lee, J. E. Lee, S. Lee, C.-H. Kim, D. W. Kim, E.-K. Jo, et al.
Role of microglial IKK{beta} in kainic acid-induced hippocampal neuronal cell death
Brain,
November 1, 2008;
131(11):
3019 - 3033.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-J. Wu and M. Zhuo
Resting Microglial Motility Is Independent of Synaptic Plasticity in Mammalian Brain
J Neurophysiol,
April 1, 2008;
99(4):
2026 - 2032.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Beck, R. Penner, and A. Fleig
Lipopolysaccharide-induced down-regulation of Ca2+ release-activated Ca2+ currents (ICRAC) but not Ca2+-activated TRPM4-like currents (ICAN) in cultured mouse microglial cells
J. Physiol.,
January 15, 2008;
586(2):
427 - 439.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Tropel, N. Platet, J.-C. Platel, D. Noel, M. Albrieux, A.-L. Benabid, and F. Berger
Functional Neuronal Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells
Stem Cells,
December 1, 2006;
24(12):
2868 - 2876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Porcheray, C. Leone, B. Samah, A.-C. Rimaniol, N. Dereuddre-Bosquet, and G. Gras
Glutamate metabolism in HIV-infected macrophages: implications for the CNS
Am J Physiol Cell Physiol,
October 1, 2006;
291(4):
C618 - C626.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J. Liu, A. Kalous, E. L. Werry, and M. R. Bennett
Purine Release from Spinal Cord Microglia after Elevation of Calcium by Glutamate
Mol. Pharmacol.,
September 1, 2006;
70(3):
851 - 859.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Dallas, D. S. Miller, and R. Bendayan
Multidrug resistance-associated proteins: expression and function in the central nervous system.
Pharmacol. Rev.,
June 1, 2006;
58(2):
140 - 161.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kajitani, H. Yamaguchi, Y. Dan, M. Furuichi, D. Kang, and Y. Nakabeppu
MTH1, an Oxidized Purine Nucleoside Triphosphatase, Suppresses the Accumulation of Oxidative Damage of Nucleic Acids in the Hippocampal Microglia during Kainate-Induced Excitotoxicity
J. Neurosci.,
February 8, 2006;
26(6):
1688 - 1698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. E. Jensen
Role of Glutamate Receptors in Periventricular Leukomalacia
J Child Neurol,
December 1, 2005;
20(12):
950 - 959.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y. S. Shibakawa, Y. Sasaki, Y. Goshima, N. Echigo, Y. Kamiya, K. Kurahashi, Y. Yamada, and T. Andoh
Effects of ketamine and propofol on inflammatory responses of primary glial cell cultures stimulated with lipopolysaccharide
Br. J. Anaesth.,
December 1, 2005;
95(6):
803 - 810.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Stuckey, D. C. Anthony, J. P. Lowe, J. Miller, W. M. Palm, P. Styles, V. H. Perry, A. M. Blamire, and N. R. Sibson
Detection of the inhibitory neurotransmitter GABA in macrophages by magnetic resonance spectroscopy
J. Leukoc. Biol.,
August 1, 2005;
78(2):
393 - 400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Suo, M. Wu, B. A. Citron, G. T. Wong, and B. W. Festoff
Abnormality of G-Protein-Coupled Receptor Kinases at Prodromal and Early Stages of Alzheimer's Disease: An Association with Early {beta}-Amyloid Accumulation
J. Neurosci.,
March 31, 2004;
24(13):
3444 - 3452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Glezer, H. Zekki, C. Scavone, and S. Rivest
Modulation of the Innate Immune Response by NMDA Receptors Has Neuropathological Consequences
J. Neurosci.,
December 3, 2003;
23(35):
11094 - 11103.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Ayoub and A. K. Salm
Increased Morphological Diversity of Microglia in the Activated Hypothalamic Supraoptic Nucleus
J. Neurosci.,
August 27, 2003;
23(21):
7759 - 7766.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Furuta, M. Noda, S. O. Suzuki, Y. Goto, Y. Kanahori, J. D. Rothstein, and T. Iwaki
Translocation of Glutamate Transporter Subtype Excitatory Amino Acid Carrier 1 Protein in Kainic Acid-Induced Rat Epilepsy
Am. J. Pathol.,
August 1, 2003;
163(2):
779 - 787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hoffmann, O. Kann, C. Ohlemeyer, U.-K. Hanisch, and H. Kettenmann
Elevation of Basal Intracellular Calcium as a Central Element in the Activation of Brain Macrophages (Microglia): Suppression of Receptor-Evoked Calcium Signaling and Control of Release Function
J. Neurosci.,
June 1, 2003;
23(11):
4410 - 4419.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Lee, S. Dallas, M. Hong, and R. Bendayan
Drug Transporters in the Central Nervous System: Brain Barriers and Brain Parenchyma Considerations
Pharmacol. Rev.,
December 1, 2001;
53(4):
569 - 596.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Tikka and J. E. Koistinaho
Minocycline Provides Neuroprotection Against N-Methyl-D-aspartate Neurotoxicity by Inhibiting Microglia
J. Immunol.,
June 15, 2001;
166(12):
7527 - 7533.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Tikka, B. L. Fiebich, G. Goldsteins, R. Keinanen, and J. Koistinaho
Minocycline, a Tetracycline Derivative, Is Neuroprotective against Excitotoxicity by Inhibiting Activation and Proliferation of Microglia
J. Neurosci.,
April 15, 2001;
21(8):
2580 - 2588.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
