 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, May 15, 2000, 20(10):3830-3842
Effect of Lesions of the Ventrolateral Preoptic Nucleus on NREM and REM Sleep
Jun Lu1, Mary Ann Greco2, Priyattam Shiromani2, and Clifford B. Saper1, 3 1 Department of Neurology and 3 Program in Neuroscience, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, and 2 Department of Psychiatry, Brockton Veterans Administration Hospital, Harvard Medical School, Boston, Massachusetts 02115
Neurons in the ventrolateral preoptic nucleus (VLPO) in rats show c-fos activation after sleep and provide GABAergic innervation of the major monoamine arousal systems, suggesting that they may be a necessary part of the brain circuitry that produces sleep. We examined the effects on sleep behavior in rats of cell-specific damage to the VLPO by microinjection of ibotenic acid. Severe lesions of the central cell cluster of the VLPO (~80-90% cell loss bilaterally) caused a 60-70% decrease in delta power and a 50-60% decrease in nonrapid-eye-movement (NREM) sleep time (p < 0.001). The number of remaining Fos-immunoreactive neurons in the VLPO cell cluster was linearly related to NREM sleep time (r = 0.77; p < 0.001) and total electroencephalogram delta power (r = 0.79; p < 0.001) but not to rapid-eye-movement (REM) sleep (r = 0.35; p > 0.10). Lesions in the region containing scattered VLPO neurons medial or dorsal to the cell cluster caused smaller changes in NREM sleep time (24.5 or 15%, respectively) but were more closely associated with loss of REM sleep (r = 0.74; p < 0.01). The insomnia caused by bilateral VLPO lesions persisted for at least 3 weeks. Lesions of the VLPO caused no change in mean body temperature or its circadian variation; after small lesions of the ventromedial preoptic nucleus, body temperature showed normal circadian variation but a wider temperature range, and sleep behavior was not affected. These experiments delineate distinct preoptic sites with primary effects on the regulation of NREM sleep, REM sleep, and body temperature.
Key words: preoptic area; hypothalamus; REM sleep; NREM sleep; thermoregulation; circadian rhythms
Copyright © 2000 Society for Neuroscience 0270-6474/00/20103830-13$05.00/0
This article has been cited by other articles:
C. G. Diniz Behn, N. Kopell, E. N. Brown, T. Mochizuki, and T. E. Scammell
Delayed Orexin Signaling Consolidates Wakefulness and Sleep: Physiology and Modeling
J Neurophysiol, June 1, 2008; 99(6): 3090 - 3103.
[Abstract] [Full Text] [PDF]
S. Kaur, A. Junek, M. A. Black, and K. Semba
Effects of Ibotenate and 192IgG-Saporin Lesions of the Nucleus Basalis Magnocellularis/Substantia Innominata on Spontaneous Sleep and Wake States and on Recovery Sleep after Sleep Deprivation in Rats
J. Neurosci., January 9, 2008; 28(2): 491 - 504.
[Abstract] [Full Text] [PDF]
J. Best, C. Diniz Behn, G. R. Poe, and V. Booth
Neuronal Models for Sleep-Wake Regulation and Synaptic Reorganization in the Sleeping Hippocampus
J Biol Rhythms, June 1, 2007; 22(3): 220 - 232.
[Abstract] [PDF]
C. G. D. Behn, E. N. Brown, T. E. Scammell, and N. J. Kopell
Mathematical Model of Network Dynamics Governing Mouse Sleep-Wake Behavior
J Neurophysiol, June 1, 2007; 97(6): 3828 - 3840.
[Abstract] [Full Text] [PDF]
D. A. Prober, J. Rihel, A. A. Onah, R.-J. Sung, and A. F. Schier
Hypocretin/Orexin Overexpression Induces An Insomnia-Like Phenotype in Zebrafish
J. Neurosci., December 20, 2006; 26(51): 13400 - 13410.
[Abstract] [Full Text] [PDF]
P. M. Fuller, J. J. Gooley, and C. B. Saper
Neurobiology of the Sleep-Wake Cycle: Sleep Architecture, Circadian Regulation, and Regulatory Feedback.
J Biol Rhythms, December 1, 2006; 21(6): 482 - 493.
[Abstract] [PDF]
M. Ruger, M. C. M. Gordijn, D. G. M. Beersma, B. de Vries, and S. Daan
Time-of-day-dependent effects of bright light exposure on human psychophysiology: comparison of daytime and nighttime exposure
Am J Physiol Regulatory Integrative Comp Physiol, May 1, 2006; 290(5): R1413 - R1420.
[Abstract] [Full Text] [PDF]
I. Gvilia, A. Turner, D. McGinty, and R. Szymusiak
Preoptic area neurons and the homeostatic regulation of rapid eye movement sleep.
J. Neurosci., March 15, 2006; 26(11): 3037 - 3044.
[Abstract] [Full Text] [PDF]
M. D. Schwartz and L. Smale
Individual Differences in Rhythms of Behavioral Sleep and Its Neural Substrates in Nile Grass Rats
J Biol Rhythms, December 1, 2005; 20(6): 526 - 537.
[Abstract] [PDF]
F. C. Baker, C. Angara, R. Szymusiak, and D. McGinty
Persistence of sleep-temperature coupling after suprachiasmatic nuclei lesions in rats
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2005; 289(3): R827 - R838.
[Abstract] [Full Text] [PDF]
F. C. Baker, S. Shah, D. Stewart, C. Angara, H. Gong, R. Szymusiak, M. R. Opp, and D. McGinty
Interleukin 1{beta} enhances non-rapid eye movement sleep and increases c-Fos protein expression in the median preoptic nucleus of the hypothalamus
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2005; 288(4): R998 - R1005.
[Abstract] [Full Text] [PDF]
C.-C. Lo, T. Chou, T. Penzel, T. E. Scammell, R. E. Strecker, H. E. Stanley, and P. Ch. Ivanov
Common scale-invariant patterns of sleep-wake transitions across mammalian species
PNAS, December 14, 2004; 101(50): 17545 - 17548.
[Abstract] [Full Text] [PDF]
M. Ouyang, K. Hellman, T. Abel, and S. A. Thomas
Adrenergic Signaling Plays a Critical Role in the Maintenance of Waking and in the Regulation of REM Sleep
J Neurophysiol, October 1, 2004; 92(4): 2071 - 2082.
[Abstract] [Full Text] [PDF]
D. McGinty, A. Metes, Md. N. Alam, D. Megirian, D. Stewart, and R. Szymusiak
Preoptic hypothalamic warming suppresses laryngeal dilator activity during sleep
Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2004; 286(6): R1129 - R1137.
[Abstract] [Full Text] [PDF]
H. Gong, D. McGinty, R. Guzman-Marin, K.-T. Chew, D. Stewart, and R. Szymusiak
Activation of c-fos in GABAergic neurones in the preoptic area during sleep and in response to sleep deprivation
J. Physiol., May 1, 2004; 556(3): 935 - 946.
[Abstract] [Full Text] [PDF]
B. Saint-Mleux, E. Eggermann, A. Bisetti, L. Bayer, D. Machard, B. E. Jones, M. Muhlethaler, and M. Serafin
Nicotinic Enhancement of the Noradrenergic Inhibition of Sleep-Promoting Neurons in the Ventrolateral Preoptic Area
J. Neurosci., January 7, 2004; 24(1): 63 - 67.
[Abstract] [Full Text] [PDF]
T. C. Chou, T. E. Scammell, J. J. Gooley, S. E. Gaus, C. B. Saper, and J. Lu
Critical Role of Dorsomedial Hypothalamic Nucleus in a Wide Range of Behavioral Circadian Rhythms
J. Neurosci., November 19, 2003; 23(33): 10691 - 10702.
[Abstract] [Full Text] [PDF]
J. J. Gooley, J. Lu, D. Fischer, and C. B. Saper
A Broad Role for Melanopsin in Nonvisual Photoreception
J. Neurosci., August 6, 2003; 23(18): 7093 - 7106.
[Abstract] [Full Text] [PDF]
S.-i. Matsuo, I.-S. Jang, J. Nabekura, and N. Akaike
alpha 2-Adrenoceptor-Mediated Presynaptic Modulation of GABAergic Transmission in Mechanically Dissociated Rat Ventrolateral Preoptic Neurons
J Neurophysiol, March 1, 2003; 89(3): 1640 - 1648.
[Abstract] [Full Text] [PDF]
J. Lu, A. A. Bjorkum, M. Xu, S. E. Gaus, P. J. Shiromani, and C. B. Saper
Selective Activation of the Extended Ventrolateral Preoptic Nucleus during Rapid Eye Movement Sleep
J. Neurosci., June 1, 2002; 22(11): 4568 - 4576.
[Abstract] [Full Text] [PDF]
D.-J. Dijk and S. W. Lockley
Functional Genomics of Sleep and Circadian Rhythm: Invited Review: Integration of human sleep-wake regulation and circadian rhythmicity
J Appl Physiol, February 1, 2002; 92(2): 852 - 862.
[Abstract] [Full Text] [PDF]
T. C. Chou, A. A. Bjorkum, S. E. Gaus, J. Lu, T. E. Scammell, and C. B. Saper
Afferents to the Ventrolateral Preoptic Nucleus
J. Neurosci., February 1, 2002; 22(3): 977 - 990.
[Abstract] [Full Text] [PDF]
J. Lu, Y.-H. Zhang, T. C. Chou, S. E. Gaus, J. K. Elmquist, P. Shiromani, and C. B. Saper
Contrasting Effects of Ibotenate Lesions of the Paraventricular Nucleus and Subparaventricular Zone on Sleep-Wake Cycle and Temperature Regulation
J. Neurosci., July 1, 2001; 21(13): 4864 - 4874.
[Abstract] [Full Text] [PDF]
T. E. Scammell, I. V. Estabrooke, M. T. McCarthy, R. M. Chemelli, M. Yanagisawa, M. S. Miller, and C. B. Saper
Hypothalamic Arousal Regions Are Activated during Modafinil-Induced Wakefulness
J. Neurosci., November 15, 2000; 20(22): 8620 - 8628.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|