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The Journal of Neuroscience, 0000, 20:RC74:1-5

RAPID COMMUNICATION
Identification of a 24 kDa Phosphoprotein Associated with an Intermediate Stage of Memory in Hermissenda

Terry Crow and Juan-Juan Xue-Bian

Department of Neurobiology and Anatomy, University of Texas Medical School, Houston, Texas 77030

A requirement for protein synthesis is a critical feature in dissociating different phases of memory. However, in examples of cellular and synaptic plasticity in which an early or intermediate requirement for protein synthesis has been implicated, specific proteins have not been identified. Here we report the identification of a 24 kDa phosphoprotein (CSP24) associated with an intermediate stage of memory, distinct from short-term memory, detected after one-trial conditioning of Hermissenda. CSP24, initially identified from ³²PO₄-labeled proteins resolved by two dimensional (2-D) PAGE, was excised from multiple Coomassie blue-stained 2-D gels and subjected to reverse phase HPLC and automated sequence analysis. The sequenced peptides exhibited a homology to the -thymosin family of actin-binding protein. Anti-CSP24 antibody recognized CSP24 on 1- and 2-D gels by Western blot analysis. Labeled CSP24 immunoprecipitated with anti-CSP24 antibody revealed that significantly more ³²PO₄ was incorporated in preparations that received one-trial conditioning compared with unpaired controls. In contrast, labeled CSP24 immunoprecipitated with anti-CSP24 from conditioned and unpaired control preparations receiving a procedure that only produced short-term enhanced excitability did not exhibit differences in ³²PO₄ incorporation into the immunoprecipitates. These results show that a specific identified phosphoprotein is associated with an intermediate stage of memory for one-trial conditioning in Hermissenda.

Key words: intermediate memory; phosphoprotein; one-trial conditioning; immunoprecipitation; associative learning; Hermissenda

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Copyright © 0000 Society for Neuroscience  0270-6474/00/$05.00/0

This article has been cited by other articles:

				T. Crow and J. J. Xue-Bian One-Trial In Vitro Conditioning Regulates a Cytoskeletal-Related Protein (CSP24) in the Conditioned Stimulus Pathway of Hermissenda J. Neurosci., December 15, 2002; 22(24): 10514 - 10518. [Abstract] [Full Text] [PDF]

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