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The Journal of Neuroscience, 0000, 20:RC76:1-6
RAPID COMMUNICATION
Chemical Stimulation of Synaptosomes Modulates
 -Ca2+/Calmodulin-Dependent Protein Kinase II mRNA
Association to Polysomes
Claudia
Bagni1, 2,
Liliana
Mannucci1,
Carlos
G.
Dotti2, and
Francesco
Amaldi1
1 Dipartimento di Biologia, Università di Roma
"Tor Vergata," 00133 Rome, Italy, and 2 European
Molecular Biology Laboratory, Cell Biology and Biophysics Program,
69012 Heidelberg, Germany
The presence of specific mRNAs in dendrites and at synapses is well
established, but a direct and reliable demonstration that they are
associated with polysomes is still missing. To address this point we
analyzed the polysomal association of the mRNAs for the  -subunit of
Ca2+/calmodulin-dependent protein kinase II
(-CaMKII), for type 1 inositol 1,4,5-trisphosphate receptor
(InsP3R1) and for the activity-regulated cytoskeleton-associated
protein (Arc) in a synaptosomal preparation devoid of contaminating
material from neuronal and glial perikarya. We show that a fraction of
-CaMKII, InsP3R1, and Arc mRNAs present in synaptosomes is indeed
associated with polysomes. Moreover, we show that polysomal association
of -CaMKII mRNA, but not InsP3R1 and Arc mRNAs, increases with
depolarization of the synaptosomal membrane. Finally, we show that the
synthesis of -CaMKII protein increases with stimulation. Dendritic
mRNA recruitment onto polysomes in response to synaptic stimulation
might represent one of the mechanisms underlying the processes of
learning and memory.
Key words:
-CaMKII; synaptosomes; chemical stimulation of
synaptosomes; translation regulation; polysome/mRNA distribution of
mRNA; synaptic plasticity
Copyright © 0000 Society for Neuroscience 0270-6474/00/$05.00/0
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