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The Small GTP-Binding Protein TC10 Promotes Nerve Elongation in
Neuronal Cells, and Its Expression Is induced during Nerve Regeneration
in Rats
Katsuhisa
Tanabe1, 2, 3, 6,
Taro
Tachibana4,
Toshihide
Yamashita3,
Yong Ho
Che2, 3, 6,
Yoshihiro
Yoneda4,
Takahiro
Ochi5,
Masaya
Tohyama3, 6,
Hideki
Yoshikawa2, and
Hiroshi
Kiyama1, 6
1 Department of Anatomy, Asahikawa Medical College,
Asahikawa, 078-8510 Japan, Departments of 2 Orthopaedic
Surgery, 3 Anatomy and Neuroscience, 4 Anatomy
and Cell Biology, and 5 Applied Medical Engineering,
Graduate School of Medicine, Osaka University, Osaka, 565-0871 Japan, and 6 Core Research for Evolutional Science
and Technology (CREST), Japan Science and Technology (JST), Kawaguchi,
Saitama, 332-0012 Japan
We have made a rat cDNA library using nerve-transected hypoglossal
nuclei. Using this library, we performed expressed-sequence tag
analysis coupled with in situ hybridization to identify
genes whose expression is altered in response to nerve injury. In this gene screening, a member of Rho family GTPases, TC10, which had not yet
been characterized in neuronal cells, was identified. TC10 mRNA
expression was very low in normal motor neurons; however, axotomy
induced its expression dramatically. Other family members such as RhoA,
Rac1, and Cdc42 were moderately expressed in normal motor neurons and
showed slight upregulation after axotomy. The expression level of TC10
mRNA was low in the embryonic brain and gradually increased with
development. However, the expression of TC10 mRNA in the adult brain
was lower and more restricted than that of RhoA, Rac1, and Cdc42.
Cultured dorsal root ganglia exhibited dramatic neurite extension
secondary to adenovirus-mediated expression of TC10. It can be
concluded that although TC10 expression is lower in developing and
mature motor neurons compared with other Rho family members, TC10
expression is induced by nerve injury to play a crucial role in nerve
regeneration, particularly neurite elongation, in cooperation with
other family members.
Key words:
Rho; nerve regeneration; cytoskeleton; axotomy; adenovirus; hypoglossal motor neuron; DRG
Copyright © 2000 Society for Neuroscience 0270-6474/00/20114138-07$05.00/0
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