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The Journal of Neuroscience, July 1, 2000, 20(13):4904-4911
Immunoisolation of GABA-Specific Synaptic Vesicles Defines a Functionally Distinct Subset of Synaptic Vesicles
Shigeo Takamori, Dietmar Riedel, and Reinhard Jahn Department of Neurobiology, Max-Planck-Institute for Biophysical Chemistry, D-37077 Göttingen, Germany
Synaptic vesicles from mammalian brain are among the best characterized trafficking organelles. However, so far it has not been possible to characterize vesicle subpopulations that are specific for a given neurotransmitter. Taking advantage of the recent molecular characterization of vesicular neurotransmitter transporters, we have used an antibody specific for the vesicular GABA transporter (VGAT) to isolate GABA-specific synaptic vesicles. The isolated vesicles are of exceptional purity as judged by electron microscopy. Immunoblotting revealed that isolated vesicles contain most of the major synaptic vesicle proteins in addition to VGAT and are devoid of vesicular monoamine and acetylcholine transporters. The vesicles are 10-fold enriched in GABA uptake activity when compared with the starting vesicle fraction. Furthermore, glutamate uptake activity and glutamate-induced but not chloride-induced acidification are selectively lost during immunoisolation. We conclude that the population of GABA-containing synaptic vesicles is separable and distinct from vesicle populations transporting other neurotransmitters.
Key words: GABA uptake; glutamate uptake; neurotransmitter phenotype; vesicular neurotransmitter uptake; synaptic vesicle proteins; vesicular GABA transporter
Copyright © 2000 Society for Neuroscience 0270-6474/00/20134904-08$05.00/0
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