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The Journal of Neuroscience, July 1, 2000, 20(13):4962-4974
Slit2 Is a Repellent for Retinal Ganglion Cell Axons
Simone P. Niclou, Li Jia, and Jonathan A. Raper Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104
We set out to isolate inhibitory guidance cues that affect retinal ganglion cell (RGC) axons in vitro and that could potentially be involved in RGC pathfinding decisions. Here we describe the biochemical purification of an RGC growth cone collapsing factor from bovine brain membranes and its identification as Slit2. Recombinant human Slit2 collapses and repels RGC growth cones from all quadrants of the chick retina. In the developing mouse visual system, slit2 is expressed in the eye, in the optic stalk, and in the ventral diencephalon. Slit2 expression is strong in anterior ventral diencephalic structures but is absent from the ventral midline where the optic chiasm forms. The putative receptors for Slits, robo1 and robo2, are expressed in the inner retinal layer in which RGCs are located. A comparison of the expression patterns of Slit2 and retinal axon trajectories suggests that slit2 acts as a short range repellent for retinal ganglion cell axons.
Key words: Slit; Roundabout; growth cone guidance; repellent; growth cone collapse; retina; visual system development
Copyright © 2000 Society for Neuroscience 0270-6474/00/20134962-13$05.00/0
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