 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, July 1, 2000, 20(13):5144-5152
Activation of Locomotion in Adult Chronic Spinal Rats Is Achieved by Transplantation of Embryonic Raphe Cells Reinnervating a Precise Lumbar Level
Minerva Giménez y Ribotta1, Jeanine Provencher2, Delphine Feraboli-Lohnherr3, Serge Rossignol2, Alain Privat1, and Didier Orsal3 1 Institut National de la Santé et de la Recherche Médicale U336, Ecole Pratique des Hautes Etudes, Université Montpellier II, F-34095 Montpellier, France 2 Centre de Recherches en Sciences Neurologiques, Université de Montréal, Montréal, Quebec, H3C 3J7 Canada and 3 Centre National de la Recherche Scientifique EP 1848, Neurophysique et Physiologie des Systèmes Moteurs, Université Rene Descartes, 75270 Paris, France
Traumatic lesions of the spinal cord yield a loss of supraspinal control of voluntary locomotor activity, although the spinal cord contains the necessary circuitry to generate the basic locomotor pattern. In spinal rats, this network, known as central pattern generator (CPG), was shown to be sensitive to serotonergic pharmacological stimulation. In previous works we have shown that embryonic raphe cells transplanted into the sublesional cord of adult rats can reinnervate specific targets, restore the lesion-induced increase in receptor densities of neurotransmitters, promote hindlimb weight support, and trigger a locomotor activity on a treadmill without any other pharmacological treatment or training.
With the aim of discriminating whether the action of serotonin on CPG is associated to a specific level of the cord, we have transplanted embryonic raphe cells at two different levels of the sublesional cord (T9 and T11) and then performed analysis of the kinematic and EMG activity synchronously recorded during locomotion. Locomotor performances were correlated to the reinnervated level of the cord and compared to that of intact and transected nontransplanted animals. The movements expressed by T11 transplanted animals correspond to a well defined locomotor pattern comparable to that of the intact animals. On the contrary, T9 transplanted animals developed limited and disorganized movements as those of nontransplanted animals. The correlation of the locomotor performances with the level of reinnervation of the spinal cord suggests that serotonergic reinnervation of the L1-L2 level constitutes a key element in the genesis of this locomotor rhythmic activity. This is the first in vivo demonstration that transplanted embryonic raphe cells reinnervating a specific level of the cord activate a locomotor behavior.
Key words: locomotion; spinal rat; transplantation of embryonic neurons; locomotor recovery; kinematic analysis; EMG
Copyright © 2000 Society for Neuroscience 0270-6474/00/20135144-09$05.00/0
This article has been cited by other articles:
S. Giszter, M. R. Davies, A. Ramakrishnan, U. I. Udoekwere, and W. J. Kargo
Trunk Sensorimotor Cortex Is Essential for Autonomous Weight-Supported Locomotion in Adult Rats Spinalized as P1/P2 Neonates
J Neurophysiol, August 1, 2008; 100(2): 839 - 851.
[Abstract] [Full Text] [PDF]
M. D. Kubasak, D. L. Jindrich, H. Zhong, A. Takeoka, K. C. McFarland, C. Munoz-Quiles, R. R. Roy, V. R. Edgerton, A. Ramon-Cueto, and P. E. Phelps
OEG implantation and step training enhance hindlimb-stepping ability in adult spinal transected rats
Brain, January 1, 2008; 131(1): 264 - 276.
[Abstract] [Full Text] [PDF]
F. Papastefanaki, J. Chen, A. A. Lavdas, D. Thomaidou, M. Schachner, and R. Matsas
Grafts of Schwann cells engineered to express PSA-NCAM promote functional recovery after spinal cord injury
Brain, August 1, 2007; 130(8): 2159 - 2174.
[Abstract] [Full Text] [PDF]
J. Chen, J. Wu, I. Apostolova, M. Skup, A. Irintchev, S. Kugler, and M. Schachner
Adeno-associated virus-mediated L1 expression promotes functional recovery after spinal cord injury
Brain, April 1, 2007; 130(4): 954 - 969.
[Abstract] [Full Text] [PDF]
J. M. Wilson, D. A. Dombeck, M. Diaz-Rios, R. M. Harris-Warrick, and R. M. Brownstone
Two-Photon Calcium Imaging of Network Activity in XFP-Expressing Neurons in the Mouse
J Neurophysiol, April 1, 2007; 97(4): 3118 - 3125.
[Abstract] [Full Text] [PDF]
D. Barthelemy, H. Leblond, and S. Rossignol
Characteristics and Mechanisms of Locomotion Induced by Intraspinal Microstimulation and Dorsal Root Stimulation in Spinal Cats
J Neurophysiol, March 1, 2007; 97(3): 1986 - 2000.
[Abstract] [Full Text] [PDF]
J. A. Norton and M. A. Gorassini
Changes in Cortically Related Intermuscular Coherence Accompanying Improvements in Locomotor Skills in Incomplete Spinal Cord Injury
J Neurophysiol, April 1, 2006; 95(4): 2580 - 2589.
[Abstract] [Full Text] [PDF]
J- M Nothias, T. Mitsui, J. S. Shumsky, I. Fischer, M. D. Antonacci, and M. Murray
Combined Effects of Neurotrophin Secreting Transplants, Exercise, and Serotonergic Drug Challenge Improve Function in Spinal Rats
Neurorehabil Neural Repair, December 1, 2005; 19(4): 296 - 312.
[Abstract] [PDF]
S. L. Thomas and M. A. Gorassini
Increases in Corticospinal Tract Function by Treadmill Training After Incomplete Spinal Cord Injury
J Neurophysiol, October 1, 2005; 94(4): 2844 - 2855.
[Abstract] [Full Text] [PDF]
J. Liu and L. M. Jordan
Stimulation of the Parapyramidal Region of the Neonatal Rat Brain Stem Produces Locomotor-Like Activity Involving Spinal 5-HT7 and 5-HT2A Receptors
J Neurophysiol, August 1, 2005; 94(2): 1392 - 1404.
[Abstract] [Full Text] [PDF]
J. M. Wilson, R. Hartley, D. J. Maxwell, A. J. Todd, I. Lieberam, J. A. Kaltschmidt, Y. Yoshida, T. M. Jessell, and R. M. Brownstone
Conditional Rhythmicity of Ventral Spinal Interneurons Defined by Expression of the Hb9 Homeodomain Protein
J. Neurosci., June 15, 2005; 25(24): 5710 - 5719.
[Abstract] [Full Text] [PDF]
C. Langlet, H. Leblond, and S. Rossignol
Mid-Lumbar Segments Are Needed for the Expression of Locomotion in Chronic Spinal Cats
J Neurophysiol, May 1, 2005; 93(5): 2474 - 2488.
[Abstract] [Full Text] [PDF]
K. Fouad, L. Schnell, M. B. Bunge, M. E. Schwab, T. Liebscher, and D. D. Pearse
Combining Schwann Cell Bridges and Olfactory-Ensheathing Glia Grafts with Chondroitinase Promotes Locomotor Recovery after Complete Transection of the Spinal Cord
J. Neurosci., February 2, 2005; 25(5): 1169 - 1178.
[Abstract] [Full Text] [PDF]
M. A. Oatway, Y. Chen, J. C. Bruce, G. A. Dekaban, and L. C. Weaver
Anti-CD11d Integrin Antibody Treatment Restores Normal Serotonergic Projections to the Dorsal, Intermediate, and Ventral Horns of the Injured Spinal Cord
J. Neurosci., January 19, 2005; 25(3): 637 - 647.
[Abstract] [Full Text] [PDF]
L. Campos, Z. Meng, G. Hu, D. T. W. Chiu, R. T. Ambron, and J. H. Martin
Engineering Novel Spinal Circuits to Promote Recovery after Spinal Injury
J. Neurosci., March 3, 2004; 24(9): 2090 - 2101.
[Abstract] [Full Text] [PDF]
H. Leblond, M. L'Esperance, D. Orsal, and S. Rossignol
Treadmill Locomotion in the Intact and Spinal Mouse
J. Neurosci., December 10, 2003; 23(36): 11411 - 11419.
[Abstract] [Full Text] [PDF]
G. Auda-Boucher, T. Rouaud, J. Fontaine-Perus, F. Le Grand, and M.-F. Gardahaut
Developmental Behavior of Embryonic Myogenic Progenitors Transplanted into Adult Muscle as Revealed by Desmin LacZ Recombinant Gene
J. Histochem. Cytochem., October 1, 2003; 51(10): 1255 - 1267.
[Abstract] [Full Text] [PDF]
V. Menet, M. Prieto, A. Privat, and M. G. y Ribotta
Axonal plasticity and functional recovery after spinal cord injury in mice deficient in both glial fibrillary acidic protein and vimentin genes
PNAS, July 22, 2003; 100(15): 8999 - 9004.
[Abstract] [Full Text] [PDF]
I. Strauss and A. Lev-Tov
Neural Pathways Between Sacrocaudal Afferents and Lumbar Pattern Generators in Neonatal Rats
J Neurophysiol, February 1, 2003; 89(2): 773 - 784.
[Abstract] [Full Text] [PDF]
H. Gabbay, I. Delvolve, and A. Lev-Tov
Pattern Generation in Caudal-Lumbar and Sacrococcygeal Segments of the Neonatal Rat Spinal Cord
J Neurophysiol, August 1, 2002; 88(2): 732 - 739.
[Abstract] [Full Text] [PDF]
P. Dergham, B. Ellezam, C. Essagian, H. Avedissian, W. D. Lubell, and L. McKerracher
Rho Signaling Pathway Targeted to Promote Spinal Cord Repair
J. Neurosci., August 1, 2002; 22(15): 6570 - 6577.
[Abstract] [Full Text] [PDF]
T. Takami, M. Oudega, M. L. Bates, P. M. Wood, N. Kleitman, and M. B. Bunge
Schwann Cell But Not Olfactory Ensheathing Glia Transplants Improve Hindlimb Locomotor Performance in the Moderately Contused Adult Rat Thoracic Spinal Cord
J. Neurosci., August 1, 2002; 22(15): 6670 - 6681.
[Abstract] [Full Text] [PDF]
S. Rossignol
Locomotion and its Recovery after Spinal Injury in Animal Models
Neurorehabil Neural Repair, June 1, 2002; 16(2): 201 - 206.
[PDF]
P. Branchereau, J. Chapron, and P. Meyrand
Descending 5-Hydroxytryptamine Raphe Inputs Repress the Expression of Serotonergic Neurons and Slow the Maturation of Inhibitory Systems in Mouse Embryonic Spinal Cord
J. Neurosci., April 1, 2002; 22(7): 2598 - 2606.
[Abstract] [Full Text] [PDF]
J. V. Coumans, T. T.-S. Lin, H. N. Dai, L. MacArthur, M. McAtee, C. Nash, and B. S. Bregman
Axonal Regeneration and Functional Recovery after Complete Spinal Cord Transection in Rats by Delayed Treatment with Transplants and Neurotrophins
J. Neurosci., December 1, 2001; 21(23): 9334 - 9344.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|