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The Journal of Neuroscience, July 15, 2000, 20(14):5312-5320
Properties and Plasticity of Paired-Pulse Depression at a
Central Synapse
Robert F.
Waldeck,
Alberto
Pereda, and
Donald S.
Faber
Department of Neurobiology and Anatomy, MCP-Hahnemann
University of the Health Sciences, Philadelphia, Pennsylvania 19129
Synaptic depression was studied at the axo-axonic connection
between the goldfish Mauthner axon and identified cranial relay interneurons using simultaneous presynaptic and postsynaptic recordings and a paired-pulse stimulus paradigm. We used interstimulus intervals (ISIs) ranging from 10 msec to 1 sec and a cycle time of ~5 sec. Depression ( EPSP/EPSP1) was maximal at the shorter intervals (80%)
and decreased exponentially with a  ~ 400 msec (360 ± 107 msec, mean ± SD). We found the amplitudes of the first and
second EPSP were not correlated, indicating the magnitude of depression does not depend on the amount of transmitter released by the
conditioning stimulus. At short ISIs, the latency of EPSP2 was 23%
longer than that of EPSP1 and recovered to control with ~ 400 msec, whereas rise time and decay time were not altered
significantly. The latency distribution, which is determined by the
timing of the first quantum released each trial, was used to derive
 (t), the rate of evoked exocytosis after an action potential. (t)
was biphasic, and both components were consistently delayed during
depression. Presynaptic manipulations of putative intracellular
regulatory pathways, such as Ca2+ and GTP S
injections, preferentially affected the amplitude of EPSP1 or EPSP2.
These results are not consistent with simple depletion of the available
pool of synaptic vesicles as the major mechanism underlying depression.
They rather suggest that it is attributable to a modification or
refractoriness of the release process and that there may be multiple
pathways subserving evoked exocytosis.
Key words:
paired-pulse depression; depletion; Mauthner cell; exocytosis; synaptic latency; rate of evoked transmitter release; cranial relay interneuron; secretory machinery; metaplasticity
Copyright © 2000 Society for Neuroscience 0270-6474/00/20145312-09$05.00/0
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