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The Journal of Neuroscience, 0000, 20:RC84:1-5
RAPID COMMUNICATION
Rapsynoid/Partner of Inscuteable Controls Asymmetric Division of
Larval Neuroblasts in Drosophila
Marie-Laure
Parmentier1,
Daniel
Woods2,
Steve
Greig1,
Phu G.
Phan1,
Anna
Radovic2,
Peter
Bryant2, and
Cahir J.
O'Kane1
1 Department of Genetics, University of Cambridge,
Cambridge CB2 3EH, United Kingdom, and 2 Developmental
Biology Center, University of California, Irvine, California 92697
Asymmetric cell division generates daughter cells with different
developmental fates. In Drosophila neuroblasts,
asymmetric divisions are characterized by (1) a difference in size
between the two daughter cells and (2) an asymmetric distribution of
cell fate determinants, including Prospero and Numb, between the two daughter cells. In embryonic neuroblasts, the asymmetric localization of cell fate determinants is under the control of the protein Inscuteable (Insc), which is itself localized asymmetrically as an
apical crescent. Here, we describe a new Drosophila
protein, Rapsynoid (Raps), which interacts in a two-hybrid assay with
the signal transduction protein G i. We show that Raps is localized asymmetrically in dividing larval neuroblasts and colocalizes with
Insc. Moreover, in raps mutants, the asymmetric
divisions of neuroblasts are altered: (1) Insc is no longer
asymmetrically localized in the dividing neuroblast; and (2) the
neuroblast division produces two daughter cells of similar sizes.
However, the morphologically symmetrical divisions of
raps neuroblasts still lead to daughter cells with
different fates, as shown by differences in gene expression. Our data
show that Raps is a novel protein involved in the control of asymmetric
divisions of neuroblasts.
Key words:
neuroblast; asymmetric division; development; G-protein; tetratricopeptide repeat; GoLoco motif
Copyright © 0000 Society for Neuroscience 0270-6474/00/$05.00/0
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