QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (127) Citing Articles via Google Scholar Google Scholar Articles by Kofuji, P. Articles by Newman, E. A. Search for Related Content PubMed PubMed Citation Articles by Kofuji, P. Articles by Newman, E. A.

 Previous Article  |  Next Article 

The Journal of Neuroscience, August 1, 2000, 20(15):5733-5740

Genetic Inactivation of an Inwardly Rectifying Potassium Channel (Kir4.1 Subunit) in Mice: Phenotypic Impact in Retina

Paulo Kofuji¹, Paul Ceelen¹, Kathleen R. Zahs², Leslie W. Surbeck¹, Henry A. Lester³, and Eric A. Newman¹

Departments of ¹ Neuroscience and ² Physiology, University of Minnesota, Minneapolis, Minnesota 55455, and ³ Division of Biology, California Institute of Technology, Pasadena, California 91125

The inwardly rectifying potassium channel Kir4.1 has been suggested to underlie the principal K⁺ conductance of mammalian Müller cells and to participate in the generation of field potentials and regulation of extracellular K⁺ in the retina. To further assess the role of Kir4.1 in the retina, we generated a mouse line with targeted disruption of the Kir4.1 gene (Kir4.1 /). Müller cells from Kir4.1 / mice were not labeled with an anti-Kir4.1 antibody, although they appeared morphologically normal when stained with an anti-glutamine synthetase antibody. In contrast, in Müller cells from wild-type littermate (Kir4.1 +/+) mice, Kir4.1 was present and localized to the proximal endfeet and perivascular processes. In situ whole-cell patch-clamp recordings showed a 10-fold increase in the input resistance and a large depolarization of Kir4.1 / Müller cells compared with Kir4.1 +/+ cells. The slow PIII response of the light-evoked electroretinogram (ERG), which is generated by K⁺ fluxes through Müller cells, was totally absent in retinas from Kir4.1 / mice. The b-wave of the ERG, in contrast, was spared in the null mice. Overall, these results indicate that Kir4.1 is the principal K⁺ channel subunit expressed in mouse Müller glial cells. The highly regulated localization and the functional properties of Kir4.1 in Müller cells suggest the involvement of this channel in the regulation of extracellular K⁺ in the mouse retina.

Key words: Müller cell; inwardly rectifying potassium channel; Kir4.1; retina; null mouse; glia; electroretinogram; slow PIII response; b-wave; astrocyte

---

Copyright © 2000 Society for Neuroscience  0270-6474/00/20155733-08$05.00/0

This article has been cited by other articles:

				I. Iandiev, A. Wurm, M. Hollborn, P. Wiedemann, C. Grimm, C. E. Reme, A. Reichenbach, T. Pannicke, and A. Bringmann Muller Cell Response to Blue Light Injury of the Rat Retina Invest. Ophthalmol. Vis. Sci., August 1, 2008; 49(8): 3559 - 3567. [Abstract] [Full Text] [PDF]

				M. T. Pardue, A. E. Faulkner, A. Fernandes, H. Yin, F. Schaeffel, R. W. Williams, N. Pozdeyev, and P. M. Iuvone High Susceptibility to Experimental Myopia in a Mouse Model with a Retinal ON Pathway Defect Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 706 - 712. [Abstract] [Full Text] [PDF]

				S. Machida, D. Raz-Prag, R. N. Fariss, P. A. Sieving, and R. A. Bush Photopic ERG Negative Response from Amacrine Cell Signaling in RCS Rat Retinal Degeneration Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 442 - 452. [Abstract] [Full Text] [PDF]

				R. G. Gregg, M. Kamermans, J. Klooster, P. D. Lukasiewicz, N. S. Peachey, K. A. Vessey, and M. A. McCall Nyctalopin Expression in Retinal Bipolar Cells Restores Visual Function in a Mouse Model of Complete X-Linked Congenital Stationary Night Blindness J Neurophysiol, November 1, 2007; 98(5): 3023 - 3033. [Abstract] [Full Text] [PDF]

				B. Djukic, K. B. Casper, B. D. Philpot, L.-S. Chin, and K. D. McCarthy Conditional Knock-Out of Kir4.1 Leads to Glial Membrane Depolarization, Inhibition of Potassium and Glutamate Uptake, and Enhanced Short-Term Synaptic Potentiation J. Neurosci., October 17, 2007; 27(42): 11354 - 11365. [Abstract] [Full Text] [PDF]

				J. Ruiz-Ederra, H. Zhang, and A. S. Verkman Evidence against Functional Interaction between Aquaporin-4 Water Channels and Kir4.1 Potassium Channels in Retinal Muller Cells J. Biol. Chem., July 27, 2007; 282(30): 21866 - 21872. [Abstract] [Full Text] [PDF]

				M. R. Metea and E. A. Newman Signalling within the neurovascular unit in the mammalian retina Exp Physiol, July 1, 2007; 92(4): 635 - 640. [Abstract] [Full Text] [PDF]

				M. R. Metea, P. Kofuji, and E. A. Newman Neurovascular Coupling Is Not Mediated by Potassium Siphoning from Glial Cells J. Neurosci., March 7, 2007; 27(10): 2468 - 2471. [Abstract] [Full Text] [PDF]

				A. Wurm, T. Pannicke, I. Iandiev, E. Buhner, U.-C. Pietsch, A. Reichenbach, P. Wiedemann, S. Uhlmann, and A. Bringmann Changes in Membrane Conductance Play a Pathogenic Role in Osmotic Glial Cell Swelling in Detached Retinas Am. J. Pathol., December 1, 2006; 169(6): 1990 - 1998. [Abstract] [Full Text] [PDF]

				I. Iandiev, O. Uckermann, T. Pannicke, A. Wurm, S. Tenckhoff, U.-C. Pietsch, A. Reichenbach, P. Wiedemann, A. Bringmann, and S. Uhlmann Glial Cell Reactivity in a Porcine Model of Retinal Detachment Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 2161 - 2171. [Abstract] [Full Text] [PDF]

				L. Y. Marmorstein, J. Wu, P. McLaughlin, J. Yocom, M. O. Karl, R. Neussert, S. Wimmers, J. B. Stanton, R. G. Gregg, O. Strauss, et al. The Light Peak of the Electroretinogram Is Dependent on Voltage-gated Calcium Channels and Antagonized by Bestrophin (Best-1) J. Gen. Physiol., April 24, 2006; 127(5): 577 - 589. [Abstract] [Full Text] [PDF]

				C. Neusch, N. Papadopoulos, M. Muller, I. Maletzki, S. M. Winter, J. Hirrlinger, M. Handschuh, M. Bahr, D. W. Richter, F. Kirchhoff, et al. Lack of the Kir4.1 Channel Subunit Abolishes K+ Buffering Properties of Astrocytes in the Ventral Respiratory Group: Impact on Extracellular K+ Regulation J Neurophysiol, March 1, 2006; 95(3): 1843 - 1852. [Abstract] [Full Text] [PDF]

				T. Pannicke, I. Iandiev, A. Wurm, O. Uckermann, F. vom Hagen, A. Reichenbach, P. Wiedemann, H.-P. Hammes, and A. Bringmann Diabetes Alters Osmotic Swelling Characteristics and Membrane Conductance of Glial Cells in Rat Retina Diabetes, March 1, 2006; 55(3): 633 - 639. [Abstract] [Full Text] [PDF]

				J. A. Brzezinski IV, N. L. Brown, A. Tanikawa, R. A. Bush, P. A. Sieving, M. H. Vitaterna, J. S. Takahashi, and T. Glaser Loss of Circadian Photoentrainment and Abnormal Retinal Electrophysiology in Math5 Mutant Mice Invest. Ophthalmol. Vis. Sci., July 1, 2005; 46(7): 2540 - 2551. [Abstract] [Full Text] [PDF]

				D. De Saint Jan and G. L. Westbrook Detecting Activity in Olfactory Bulb Glomeruli with Astrocyte Recording J. Neurosci., March 16, 2005; 25(11): 2917 - 2924. [Abstract] [Full Text] [PDF]

				S. C. Hebert, G. Desir, G. Giebisch, and W. Wang Molecular Diversity and Regulation of Renal Potassium Channels Physiol Rev, January 1, 2005; 85(1): 319 - 371. [Abstract] [Full Text] [PDF]

				D. K. Binder, M. C. Papadopoulos, P. M. Haggie, and A. S. Verkman In Vivo Measurement of Brain Extracellular Space Diffusion by Cortical Surface Photobleaching J. Neurosci., September 15, 2004; 24(37): 8049 - 8056. [Abstract] [Full Text] [PDF]

				N. C. Connors, M. E. Adams, S. C. Froehner, and P. Kofuji The Potassium Channel Kir4.1 Associates with the Dystrophin-Glycoprotein Complex via {alpha}-Syntrophin in Glia J. Biol. Chem., July 2, 2004; 279(27): 28387 - 28392. [Abstract] [Full Text] [PDF]

				S. Uhlmann, A. Bringmann, O. Uckermann, T. Pannicke, M. Weick, E. Ulbricht, I. Goczalik, A. Reichenbach, P. Wiedemann, and M. Francke Early Glial Cell Reactivity in Experimental Retinal Detachment: Effect of Suramin Invest. Ophthalmol. Vis. Sci., September 1, 2003; 44(9): 4114 - 4122. [Abstract] [Full Text] [PDF]

				D. Yang, D. K. MacCallum, S. A. Ernst, and B. A. Hughes Expression of the Inwardly Rectifying K+ Channel Kir2.1 in Native Bovine Corneal Endothelial Cells Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3511 - 3519. [Abstract] [Full Text] [PDF]

				B. Bakall, L. Y. Marmorstein, G. Hoppe, N. S. Peachey, C. Wadelius, and A. D. Marmorstein Expression and Localization of Bestrophin during Normal Mouse Development Invest. Ophthalmol. Vis. Sci., August 1, 2003; 44(8): 3622 - 3628. [Abstract] [Full Text] [PDF]

				D. M. Menichella, D. A. Goodenough, E. Sirkowski, S. S. Scherer, and D. L. Paul Connexins Are Critical for Normal Myelination in the CNS J. Neurosci., July 2, 2003; 23(13): 5963 - 5973. [Abstract] [Full Text] [PDF]

				C. Dalloz, R. Sarig, P. Fort, D. Yaffe, A. Bordais, T. Pannicke, J. Grosche, D. Mornet, A. Reichenbach, J. Sahel, et al. Targeted inactivation of dystrophin gene product Dp71: phenotypic impact in mouse retina Hum. Mol. Genet., July 1, 2003; 12(13): 1543 - 1554. [Abstract] [Full Text] [PDF]

				D. Yang, A. Pan, A. Swaminathan, G. Kumar, and B. A. Hughes Expression and Localization of the Inwardly Rectifying Potassium Channel Kir7.1 in Native Bovine Retinal Pigment Epithelium Invest. Ophthalmol. Vis. Sci., July 1, 2003; 44(7): 3178 - 3185. [Abstract] [Full Text] [PDF]

				R. G. Gregg, S. Mukhopadhyay, S. I. Candille, S. L. Ball, M. T. Pardue, M. A. McCall, and N. S. Peachey Identification of the Gene and the Mutation Responsible for the Mouse nob Phenotype Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 378 - 384. [Abstract] [Full Text] [PDF]

				N. C. Connors and P. Kofuji Dystrophin Dp71 Is Critical for the Clustered Localization of Potassium Channels in Retinal Glial Cells J. Neurosci., June 1, 2002; 22(11): 4321 - 4327. [Abstract] [Full Text] [PDF]

				D. C. Marcus, T. Wu, P. Wangemann, and P. Kofuji KCNJ10 (Kir4.1) potassium channel knockout abolishes endocochlear potential Am J Physiol Cell Physiol, February 1, 2002; 282(2): C403 - C407. [Abstract] [Full Text] [PDF]

				C. B. Ransom, J. T. O'Neal, and H. Sontheimer Volume-Activated Chloride Currents Contribute to the Resting Conductance and Invasive Migration of Human Glioma Cells J. Neurosci., October 1, 2001; 21(19): 7674 - 7683. [Abstract] [Full Text] [PDF]

				C. Neusch, N. Rozengurt, R. E. Jacobs, H. A. Lester, and P. Kofuji Kir4.1 Potassium Channel Subunit Is Crucial for Oligodendrocyte Development and In Vivo Myelination J. Neurosci., August 1, 2001; 21(15): 5429 - 5438. [Abstract] [Full Text] [PDF]

				P. A. Sieving, M. L. Fowler, R. A. Bush, S. Machida, P. D. Calvert, D. G. Green, C. L. Makino, and C. L. McHenry Constitutive "Light" Adaptation in Rods from G90D Rhodopsin: A Mechanism for Human Congenital Nightblindness without Rod Cell Loss J. Neurosci., August 1, 2001; 21(15): 5449 - 5460. [Abstract] [Full Text] [PDF]

				E. Solessio, K. Rapp, I. Perlman, and E. M. Lasater Spermine Mediates Inward Rectification in Potassium Channels of Turtle Retinal Muller Cells J Neurophysiol, April 1, 2001; 85(4): 1357 - 1367. [Abstract] [Full Text] [PDF]

				M. Francke, F. Faude, T. Pannicke, A. Bringmann, P. Eckstein, W. Reichelt, P. Wiedemann, and A. Reichenbach Electrophysiology of Rabbit Muller (Glial) Cells in Experimental Retinal Detachment and PVR Invest. Ophthalmol. Vis. Sci., April 1, 2001; 42(5): 1072 - 1079. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help