 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, August 15, 2000, 20(16):5899-5905
Developmental Profile of the Changing Properties of NMDA Receptors at Cerebellar Mossy Fiber-Granule Cell Synapses
Laurence Cathala, Charu Misra, and Stuart Cull-Candy Department of Pharmacology, University College London, London WC1E 6BT, United Kingdom
During cerebellar development, granule cells display well characterized changes in the expression of NMDA receptor (NMDAR) NR2 subunits, switching from NR2B to NR2A and NR2C in mature cells. Although various studies, including experiments on mutant mice with one or more NR2 subunit types deleted, suggest that NR2A, NR2B, and NR2C subunits contribute to synaptic NMDARs, changes in the properties of the mossy fiber EPSC during development have not been fully evaluated. In particular, information on NMDAR EPSCs in mature animals is lacking. We have examined pharmacological and kinetic properties of NMDARs at mossy fiber-granule cell synapses from their formation to maturity [postnatal day 7 (P7)-P40 rats]. Significant changes were seen in the relative amplitudes of the non-NMDAR- and NMDAR-mediated components of the evoked EPSC and in the decay kinetics of the latter. The NMDA/non-NMDA ratio was similar at P7, P21, and P40, but showed a clear peak at P12. This change coincided with a speeding of the NMDAR EPSC decay, accompanied by a decrease in sensitivity to ifenprodil (selective NR2B-antagonist). By P21, sensitivity of the NMDAR EPSC to Mg2+ was approximately threefold less than that at P12 (IC50, 76 vs 28 µM), suggesting incorporation of the NR2C subunit. However, the predicted slowing of decay kinetics to a value more characteristic of NR2C deactivation, was not seen until P40. Our data are consistent with the known switch from NR2B to NR2A subunits during the first two postnatal weeks, but suggest a gradual incorporation of the NR2C subunit that modifies Mg2+ sensitivity and only later influences EPSC kinetics.
Key words: cerebellum; granule cells; NMDA receptors; synaptic transmission; patch-clamp; NMDA subunit expression; NR2C subunit
Copyright © 2000 Society for Neuroscience 0270-6474/00/20165899-07$05.00/0
This article has been cited by other articles:
F. Prestori, P. Rossi, B. Bearzatto, J. Laine, D. Necchi, S. Diwakar, S. N. Schiffmann, H. Axelrad, and E. D'Angelo
Altered Neuron Excitability and Synaptic Plasticity in the Cerebellar Granular Layer of Juvenile Prion Protein Knock-Out Mice with Impaired Motor Control
J. Neurosci., July 9, 2008; 28(28): 7091 - 7103.
[Abstract] [Full Text] [PDF]
X. Liu and C. Chen
Different Roles for AMPA and NMDA Receptors in Transmission at the Immature Retinogeniculate Synapse
J Neurophysiol, February 1, 2008; 99(2): 629 - 643.
[Abstract] [Full Text] [PDF]
C. Villmann and C.-M. Becker
On the Hypes and Falls in Neuroprotection: Targeting the NMDA Receptor
Neuroscientist, December 1, 2007; 13(6): 594 - 615.
[Abstract] [PDF]
C. Piochon, T. Irinopoulou, D. Brusciano, Y. Bailly, J. Mariani, and C. Levenes
NMDA Receptor Contribution to the Climbing Fiber Response in the Adult Mouse Purkinje Cell
J. Neurosci., October 3, 2007; 27(40): 10797 - 10809.
[Abstract] [Full Text] [PDF]
F. Bengtsson and H. Jorntell
Ketamine and Xylazine Depress Sensory-Evoked Parallel Fiber and Climbing Fiber Responses
J Neurophysiol, September 1, 2007; 98(3): 1697 - 1705.
[Abstract] [Full Text] [PDF]
T. Lu and L. O. Trussell
Development and Elimination of Endbulb Synapses in the Chick Cochlear Nucleus
J. Neurosci., January 24, 2007; 27(4): 808 - 817.
[Abstract] [Full Text] [PDF]
C. Lu, Z. Fu, I. Karavanov, R. P. Yasuda, B. B. Wolfe, A. Buonanno, and S. Vicini
NMDA Receptor Subtypes at Autaptic Synapses of Cerebellar Granule Neurons
J Neurophysiol, November 1, 2006; 96(5): 2282 - 2294.
[Abstract] [Full Text] [PDF]
R. J. Clarke and J. W. Johnson
NMDA Receptor NR2 Subunit Dependence of the Slow Component of Magnesium Unblock
J. Neurosci., May 24, 2006; 26(21): 5825 - 5834.
[Abstract] [Full Text] [PDF]
P. B. Sargent, C. Saviane, T. A. Nielsen, D. A. DiGregorio, and R. A. Silver
Rapid Vesicular Release, Quantal Variability, and Spillover Contribute to the Precision and Reliability of Transmission at a Glomerular Synapse
J. Neurosci., September 7, 2005; 25(36): 8173 - 8187.
[Abstract] [Full Text] [PDF]
H.-H. Chen, C.-T. Wei, Y.-R. Lin, T.-H. Chien, and M.-H. Chan
Neonatal Toluene Exposure Alters Agonist and Antagonist Sensitivity and NR2B Subunit Expression of NMDA Receptors in Cultured Cerebellar Granule Neurons
Toxicol. Sci., May 1, 2005; 85(1): 666 - 674.
[Abstract] [Full Text] [PDF]
Q. Liu and M. T. T. Wong-Riley
Postnatal developmental expressions of neurotransmitters and receptors in various brain stem nuclei of rats
J Appl Physiol, April 1, 2005; 98(4): 1442 - 1457.
[Abstract] [Full Text] [PDF]
Z. Fu, S. M Logan, and S. Vicini
Deletion of the NR2A subunit prevents developmental changes of NMDA-mEPSCs in cultured mouse cerebellar granule neurones
J. Physiol., March 15, 2005; 563(3): 867 - 881.
[Abstract] [Full Text] [PDF]
S. G. Cull-Candy and D. N. Leszkiewicz
Role of Distinct NMDA Receptor Subtypes at Central Synapses
Sci. Signal., October 19, 2004; 2004(255): re16 - re16.
[Abstract] [Full Text] [PDF]
K. J. Todd, C. A. B. Slatter, and D. W. Ali
Activation of Ionotropic Glutamate Receptors on Peripheral Axons of Primary Motoneurons Mediates Transmitter Release at the Zebrafish NMJ
J Neurophysiol, February 1, 2004; 91(2): 828 - 840.
[Abstract] [Full Text] [PDF]
L. Cathala, S. Brickley, S. Cull-Candy, and M. Farrant
Maturation of EPSCs and Intrinsic Membrane Properties Enhances Precision at a Cerebellar Synapse
J. Neurosci., July 9, 2003; 23(14): 6074 - 6085.
[Abstract] [Full Text] [PDF]
S. G. Brickley, C. Misra, M. H. S. Mok, M. Mishina, and S. G. Cull-Candy
NR2B and NR2D Subunits Coassemble in Cerebellar Golgi Cells to Form a Distinct NMDA Receptor Subtype Restricted to Extrasynaptic Sites
J. Neurosci., June 15, 2003; 23(12): 4958 - 4966.
[Abstract] [Full Text] [PDF]
C. Szinyei, O. Stork, and H.-C. Pape
Contribution of NR2B Subunits to Synaptic Transmission in Amygdaloid Interneurons
J. Neurosci., April 1, 2003; 23(7): 2549 - 2556.
[Abstract] [Full Text] [PDF]
D. E. Chapman, K. A. Keefe, and K. S. Wilcox
Evidence for Functionally Distinct Synaptic NMDA Receptors in Ventromedial Versus Dorsolateral Striatum
J Neurophysiol, January 1, 2003; 89(1): 69 - 80.
[Abstract] [Full Text] [PDF]
P. Rossi, E. Sola, V. Taglietti, T. Borchardt, F. Steigerwald, J. K. Utvik, O. P. Ottersen, G. Kohr, and E. D'Angelo
NMDA Receptor 2 (NR2) C-Terminal Control of NR Open Probability Regulates Synaptic Transmission and Plasticity at a Cerebellar Synapse
J. Neurosci., November 15, 2002; 22(22): 9687 - 9697.
[Abstract] [Full Text] [PDF]
D. Billups, Y.-B. Liu, S. Birnstiel, and N. T. Slater
NMDA Receptor-Mediated Currents in Rat Cerebellar Granule and Unipolar Brush Cells
J Neurophysiol, April 1, 2002; 87(4): 1948 - 1959.
[Abstract] [Full Text] [PDF]
G. Losi, K. Prybylowski, Z. Fu, J. H. Luo, and S. Vicini
Silent Synapses in Developing Cerebellar Granule Neurons
J Neurophysiol, March 1, 2002; 87(3): 1263 - 1270.
[Abstract] [Full Text] [PDF]
K. Futai, M. Okada, K. Matsuyama, and T. Takahashi
High-Fidelity Transmission Acquired via a Developmental Decrease in NMDA Receptor Expression at an Auditory Synapse
J. Neurosci., May 15, 2001; 21(10): 3342 - 3349.
[Abstract] [Full Text] [PDF]
G. Abdrachmanova, J. Teisinger, and L. J. Vyklicky
Axotomy-induced changes in the properties of NMDA receptor channels in rat spinal cord motoneurons
J. Physiol., January 1, 2002; 538(1): 53 - 63.
[Abstract] [Full Text] [PDF]
J. Nabekura, T. Ueno, S. Katsurabayashi, A. Furuta, N. Akaike, and M. Okada
Reduced NR2A expression and prolonged decay of NMDA receptor-mediated synaptic current in rat vagal motoneurons following axotomy
J. Physiol., March 15, 2002; 539(3): 735 - 741.
[Abstract] [Full Text] [PDF]
I. Joshi and L.-Y. Wang
Developmental profiles of glutamate receptors and synaptic transmission at a single synapse in the mouse auditory brainstem
J. Physiol., May 1, 2002; 540(3): 861 - 873.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|