 |
 Previous Article | Next Article 
The Journal of Neuroscience, August 15, 2000, 20(16):5915-5923
Membrane and Synaptic Actions of Halothane on Rat Hippocampal
Pyramidal Neurons and Inhibitory Interneurons
Koh-ichi
Nishikawa and
M. Bruce
MacIver
Neuropharmacology Laboratory, Department of Anesthesia, Stanford
University School of Medicine, Stanford, California 94305-5117
A relatively small number of inhibitory interneurons can control
the excitability and synchronization of large numbers of pyramidal
neurons in hippocampus and other cortical regions. Thus, anesthetic
modulation of interneurons could play an important role during
anesthesia. The aim of this study was to investigate effects of a
general anesthetic, halothane, on membrane and synaptic properties of
rat hippocampal interneurons. GABA receptor-mediated IPSCs were recorded with whole-cell patch-clamp techniques in visually identified CA1 pyramidal cells and interneurons located at the
border of stratum lacunosum-moleculare and stratum radiatum. Halothane
(0.35 mM  1.2 vol%) depressed evoked IPSC amplitudes recorded from both pyramidal cells and inhibitory interneurons. Also,
halothane considerably prolonged the decay time constant of evoked
IPSCs in pyramidal cells and interneurons. The frequencies of miniature
IPSCs were increased by halothane (two- to threefold) in both types of
neuron. On the other hand, halothane effects on resting membrane
potentials were variable but minimal in both types of neurons. In
current-clamp recordings, halothane depressed EPSP amplitudes
and increased IPSP amplitudes recorded from both types of
neurons. In addition, halothane increased the failure rate of
synaptically evoked action potentials. Taken together, these data
provide evidence that halothane increases GABAA
receptor-mediated synaptic inhibition between synaptically connected
interneurons and depresses excitatory transmission, similar to effects
observed in pyramidal neurons.
Key words:
GABA; miniature synaptic current; IPSC; EPSP; action
potential; Schaffer-collateral; synaptic inhibition; glutamate; CA1; anesthesia
Copyright © 2000 Society for Neuroscience 0270-6474/00/20165915-09$05.00/0
This article has been cited by other articles:
|
|
|
|
 
K. Tachibana, K. Takita, T. Hashimoto, M. Matsumoto, M. Yoshioka, and Y. Morimoto
Isoflurane Bidirectionally Modulates the Paired-Pulse Responses in the Rat Hippocampal CA1 Field In Vivo
Anesth. Analg.,
October 1, 2007;
105(4):
1006 - 1011.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Asahi, K. Hirota, R. Sasaki, Y. Mitsuaki, and S. H. Roth
Intravenous Anesthetics Are More Effective than Volatile Anesthetics on Inhibitory Pathways in Rat Hippocampal CA1.
Anesth. Analg.,
March 1, 2006;
102(3):
772 - 778.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. I. Westphalen and H. C. Hemmings Jr.
Volatile Anesthetic Effects on Glutamate versus GABA Release from Isolated Rat Cortical Nerve Terminals: Basal Release
J. Pharmacol. Exp. Ther.,
January 1, 2006;
316(1):
208 - 215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. K. Georgiev, A. Wakai, T. Kohno, T. Yamakura, and H. Baba
Actions of Norepinephrine and Isoflurane on Inhibitory Synaptic Transmission in Adult Rat Spinal Cord Substantia Gelatinosa Neurons
Anesth. Analg.,
January 1, 2006;
102(1):
124 - 128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-W. Ying and P. A. Goldstein
Propofol-Block of SK Channels in Reticular Thalamic Neurons Enhances GABAergic Inhibition in Relay Neurons
J Neurophysiol,
April 1, 2005;
93(4):
1935 - 1948.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Bieda and M. B. MacIver
Major Role For Tonic GABAA Conductances in Anesthetic Suppression of Intrinsic Neuronal Excitability
J Neurophysiol,
September 1, 2004;
92(3):
1658 - 1667.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. P. O'Boyle, V. Do, B. E. Derrick, and B. J. Claiborne
In Vivo Recordings of Long-Term Potentiation and Long-Term Depression in the Dentate Gyrus of the Neonatal Rat
J Neurophysiol,
February 1, 2004;
91(2):
613 - 622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Cheng and J. J. Kendig
Enflurane Decreases Glutamate Neurotransmission to Spinal Cord Motor Neurons by Both Pre- and Postsynaptic Actions
Anesth. Analg.,
May 1, 2003;
96(5):
1354 - 1359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. I. Westphalen and H. C. Hemmings Jr.
Selective Depression by General Anesthetics of Glutamate Versus GABA Release from Isolated Cortical Nerve Terminals
J. Pharmacol. Exp. Ther.,
March 1, 2003;
304(3):
1188 - 1196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kitamura, W. Marszalec, J. Z. Yeh, and T. Narahashi
Effects of Halothane and Propofol on Excitatory and Inhibitory Synaptic Transmission in Rat Cortical Neurons
J. Pharmacol. Exp. Ther.,
January 1, 2003;
304(1):
162 - 171.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Detsch, E. Kochs, M. Siemers, B. Bromm, and C. Vahle-Hinz
Differential effects of isoflurane on excitatory and inhibitory synaptic inputs to thalamic neurones in vivo
Br. J. Anaesth.,
August 1, 2002;
89(2):
294 - 300.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Vahle-Hinz, O. Detsch, M. Siemers, E. Kochs, and B. Bromm
Local GABAA Receptor Blockade Reverses Isoflurane's Suppressive Effects on Thalamic Neurons In Vivo
Anesth. Analg.,
June 1, 2001;
92(6):
1578 - 1584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Peinado
Immature Neocortical Neurons Exist as Extensive Syncitial Networks Linked by Dendrodendritic Electrical Connections
J Neurophysiol,
February 1, 2001;
85(2):
620 - 629.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Jenkins, E. P. Greenblatt, H. J. Faulkner, E. Bertaccini, A. Light, A. Lin, A. Andreasen, A. Viner, J. R. Trudell, and N. L. Harrison
Evidence for a Common Binding Cavity for Three General Anesthetics within the GABAA Receptor
J. Neurosci.,
March 15, 2001;
21(6):
RC136 - RC136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
