QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (40) Citing Articles via Google Scholar Google Scholar Articles by Dunaevsky, A. Articles by Connor, E. A. Search for Related Content PubMed PubMed Citation Articles by Dunaevsky, A. Articles by Connor, E. A. Pubmed/NCBI databases Substance via MeSH

 Previous Article  |  Next Article 

The Journal of Neuroscience, August 15, 2000, 20(16):6007-6012

F-Actin Is Concentrated in Nonrelease Domains at Frog Neuromuscular Junctions

Anna Dunaevsky and Elizabeth A. Connor

Department of Biology, Neuroscience and Behavior Graduate Program, University of Massachusetts, Amherst, Massachusetts 01003

To gain insight into the role of F-actin in the organization of synaptic vesicles at release sites, we examined the synaptic distribution of F-actin by using a unique synaptic preparation of frog target-deprived nerve terminals. In this preparation, imaging of the synaptic site was unobstructed by the muscle fiber cytoskeleton, allowing for the examination of hundreds of synaptic sites in their entirety in whole mounts. At target-deprived synaptic sites F-actin was distributed in a ladder-like pattern and was colocalized with -fodrin. Surprisingly, F-actin stain, which we localized to the nerve terminal itself, did not overlap a synaptic vesicle marker, suggesting that it was concentrated in nonrelease domains of nerve terminals between clusters of synaptic vesicles. These findings suggest that the majority of the presynaptic F-actin is not involved in tethering synaptic vesicles. Instead, the strategic presynaptic positioning of this cytoskeletal meshwork in nonrelease domains of the nerve terminal suggests alternate functions such as restricting synaptic vesicles to release domains, recycling synaptic vesicles, or stabilizing the nerve terminal.

Key words: F-actin; synaptic vesicles; cytoskeleton; presynaptic; -fodrin; terminal Schwann cell

---

Copyright © 2000 Society for Neuroscience  0270-6474/00/20166007-06$05.00/0

This article has been cited by other articles:

				F. Andersson, J. Jakobsson, P. Low, O. Shupliakov, and L. Brodin Perturbation of Syndapin/PACSIN Impairs Synaptic Vesicle Recycling Evoked by Intense Stimulation J. Neurosci., April 9, 2008; 28(15): 3925 - 3933. [Abstract] [Full Text] [PDF]

				H. Tokuoka and Y. Goda Myosin Light Chain Kinase Is Not a Regulator of Synaptic Vesicle Trafficking during Repetitive Exocytosis in Cultured Hippocampal Neurons. J. Neurosci., November 8, 2006; 26(45): 11606 - 11614. [Abstract] [Full Text] [PDF]

				P. Nunes, N. Haines, V. Kuppuswamy, D. J. Fleet, and B. A. Stewart Synaptic Vesicle Mobility and Presynaptic F-Actin Are Disrupted in a N-ethylmaleimide-sensitive Factor Allele of Drosophila Mol. Biol. Cell, November 1, 2006; 17(11): 4709 - 4719. [Abstract] [Full Text] [PDF]

				M. R. Wenk and P. De Camilli Inaugural Article: Protein-lipid interactions and phosphoinositide metabolism in membrane traffic: Insights from vesicle recycling in nerve terminals PNAS, June 1, 2004; 101(22): 8262 - 8269. [Abstract] [Full Text] [PDF]

				D. A. Richards, S. O. Rizzoli, and W. J. Betz Effects of wortmannin and latrunculin A on slow endocytosis at the frog neuromuscular junction J. Physiol., May 15, 2004; 557(1): 77 - 91. [Abstract] [Full Text] [PDF]

				S. O. Rizzoli and W. J. Betz The Structural Organization of the Readily Releasable Pool of Synaptic Vesicles Science, March 26, 2004; 303(5666): 2037 - 2039. [Abstract] [Full Text] [PDF]

				S. L. Sabo, A. F. Ikin, J. D. Buxbaum, and P. Greengard The Amyloid Precursor Protein and Its Regulatory Protein, FE65, in Growth Cones and Synapses In Vitro and In Vivo J. Neurosci., July 2, 2003; 23(13): 5407 - 5415. [Abstract] [Full Text] [PDF]

				J. L. Zamanian and R. B. Kelly Intersectin 1L Guanine Nucleotide Exchange Activity Is Regulated by Adjacent src Homology 3 Domains That Are Also Involved in Endocytosis Mol. Biol. Cell, April 1, 2003; 14(4): 1624 - 1637. [Abstract] [Full Text] [PDF]

				M. Holt, A. Cooke, M. M. Wu, and L. Lagnado Bulk Membrane Retrieval in the Synaptic Terminal of Retinal Bipolar Cells J. Neurosci., February 15, 2003; 23(4): 1329 - 1339. [Abstract] [Full Text] [PDF]

				W. T. Kim, S. Chang, L. Daniell, O. Cremona, G. Di Paolo, and P. De Camilli Delayed reentry of recycling vesicles into the fusion-competent synaptic vesicle pool in synaptojanin 1 knockout mice PNAS, December 24, 2002; 99(26): 17143 - 17148. [Abstract] [Full Text] [PDF]

				O. Shupliakov, O. Bloom, J. S. Gustafsson, O. Kjaerulff, P. Low, N. Tomilin, V. A. Pieribone, P. Greengard, and L. Brodin Impaired recycling of synaptic vesicles after acute perturbation of the presynaptic actin cytoskeleton PNAS, October 29, 2002; 99(22): 14476 - 14481. [Abstract] [Full Text] [PDF]

				E. Lee and P. De Camilli From the Cover: Dynamin at actin tails PNAS, January 8, 2002; 99(1): 161 - 166. [Abstract] [Full Text] [PDF]

				W. Zhang and D. L. Benson Stages of Synapse Development Defined by Dependence on F-Actin J. Neurosci., July 15, 2001; 21(14): 5169 - 5181. [Abstract] [Full Text] [PDF]

				D. E. Featherstone, W. S. Davis, R. R. Dubreuil, and K. Broadie Drosophila {alpha}- and {beta}-Spectrin Mutations Disrupt Presynaptic Neurotransmitter Release J. Neurosci., June 15, 2001; 21(12): 4215 - 4224. [Abstract] [Full Text] [PDF]

				O Cremona and P De Camilli Phosphoinositides in membrane traffic at the synapse J. Cell Sci., January 3, 2001; 114(6): 1041 - 1052. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help