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The Journal of Neuroscience, August 15, 2000, 20(16):6030-6038

Expression of GAP-43 and SCG10 mRNAs in Lateral Geniculate Nucleus of Normal and Monocularly Deprived Macaque Monkeys

Noriyuki Higo^{1, 2}, Takao Oishi¹, Akiko Yamashita³, Keiji Matsuda¹, and Motoharu Hayashi⁴

¹ Neuroscience Section, Information Science Division, Electrotechnical Laboratory, Umezono, Tsukuba, Ibaraki 305-8568, Japan, ² Department of Physiology, University of Tsukuba School of Medicine, Tennodai, Tsukuba, Ibaraki 305-8575, Japan, ³ Department of Anatomy, Nihon University School of Medicine, Oyaguchi-Kamimachi, Itabashi, Tokyo 173-8610, Japan, and ⁴ Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Kanrin, Inuyama, Aichi 484-8506, Japan

We performed nonradioactive in situ hybridization histochemistry (ISH) in the lateral geniculate nucleus (LGN) of the macaque monkey to investigate the distribution of mRNA for two growth-associated proteins, GAP-43 and SCG10. GAP-43 and SCG10 mRNAs were coexpressed in most neurons of both magnocellular layers (layers I and II) and parvocellular layers (layers III-VI). Double-labeling using nonradioactive ISH and immunofluorescence revealed that both GAP-43 and SCG10 mRNAs were coexpressed with the -subunit of type II calcium/calmodulin-dependent protein kinase, indicating that both mRNAs are expressed also in koniocellular neurons in the LGN. We also showed that GABA-immunoreactive neurons in the LGN did not contain GAP-43 and SCG10 mRNAs, indicating that neither GAP-43 nor SCG10 mRNAs were expressed in inhibitory interneurons in the LGN. GABA-immunoreactive neurons in the perigeniculate nucleus, however, contained both GAP-43 and SCG10 mRNAs, indicating that both mRNAs were expressed in inhibitory neurons in the perigeniculate nucleus, which project to relay neurons in the LGN. Furthermore, to determine whether the expression of GAP-43 and SCG10 mRNAs is regulated by visual input, we performed nonradioactive ISH in the LGN and the primary visual area of monkeys deprived of monocular visual input by intraocular injections of tetrodotoxin. Both mRNAs were downregulated in the LGN after monocular deprivation for 5 d or longer. From these results, we conclude that both GAP-43 and SCG10 mRNAs are expressed in the excitatory relay neurons of the monkey LGN in an activity-dependent manner.

Key words: GAP-43; SCG10; LGN; macaque monkey; growth-associated protein; koniocellular; GABA; monocular deprivation; in situ hybridization; activity-dependent regulation

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Copyright © 2000 Society for Neuroscience  0270-6474/00/20166030-09$05.00/0

This article has been cited by other articles:

				N. Higo, T. Oishi, A. Yamashita, K. Matsuda, and M. Hayashi Cell Type- and Region-specific Expression of Neurogranin mRNA in the Cerebral Cortex of the Macaque Monkey Cereb Cortex, October 1, 2004; 14(10): 1134 - 1143. [Abstract] [Full Text] [PDF]

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