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The Journal of Neuroscience, August 15, 2000, 20(16):6249-6255
Transduction Sites of Vagal Mechanoreceptors in the Guinea
Pig Esophagus
Vladimir P.
Zagorodnyuk and
Simon J. H.
Brookes
Department of Human Physiology and Centre for Neuroscience,
Flinders University of South Australia, Adelaide, South Australia
5001
Extrinsic afferent neurons play an essential role in both sensation
and reflex control of visceral organs, but their specialized morphological peripheral endings have never been functionally identified. Extracellular recordings were made from fine nerve trunks
running between the vagus nerve and esophagus of the guinea pig.
Mechanoreceptors, which responded to esophageal distension, fired
spontaneously, had low thresholds to circumferential stretch, and were
slowly adapting. Calibrated von Frey hairs (0.12 mN) were used to probe
the serosal surface at 100-200 sites, which were mapped on a video
image of the live preparation. Each stretch-sensitive unit had one to
three highly localized receptive fields ("hot spots"), which were
marked with Indian ink applied on the tip of the von Frey hair.
Recorded nerve trunks were then filled anterogradely, using biotinamide
in an artificial intracellular solution. Receptive fields were
consistently associated with intraganglionic laminar endings (IGLEs) in
myenteric ganglia, but not with other filled neuronal structures. The
average distance of receptive fields to IGLEs was 73 ± 14 µm
(24 receptive fields, from 12 units; n = 5),
compared to 374 ± 17 µm for 240 randomly generated sites (n = 5; p < 0.001). After
maintained probing on a single receptive field, spontaneous discharge
of units was inhibited, as were responses to distension. During adapted
discharge to maintained distension, interspike intervals were
distributed in a narrow range. This indicates that multiple receptive
fields interact to encode mechanical distortion in a graded manner.
IGLEs are specialized transduction sites of mechanosensitive vagal
afferent neurons in the guinea pig esophagus.
Key words:
vagus nerve; primary afferent; mechanoreceptor; intraganglionic laminar ending; guinea pig; esophagus; enteric nervous
system
Copyright © 2000 Society for Neuroscience 0270-6474/00/20166249-07$05.00/0
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