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The Journal of Neuroscience, 0000, 20:RC94:1-5

RAPID COMMUNICATION
Activation of Presynaptic and Postsynaptic Ryanodine-Sensitive Calcium Stores Is Required for the Induction of Long-Term Depression at GABAergic Synapses in the Neonatal Rat Hippocampus

Olivier Caillard, Yehezkel Ben-Ari, and Jean-Luc Gaïarsa

Institut de Neurobiologie de la Méditerranée (INMED), Institut National de la Santé et de la Recherche Médicale U29, B.P. 13, 13273 Marseille Cedex 09, France

The role of internal calcium stores in the induction of long-term depression at GABAergic synapses was investigated in the neonatal rat hippocampus. Whole-cell recordings of CA3 pyramidal neurons were performed on hippocampal slices from neonatal (2-4 d old) rats. In control conditions, tetanic stimulation (TS) evoked an NMDA-dependent long-term depression of GABA_A receptor-mediated postsynaptic responses (LTD_GABA-A). LTD_GABA-A was prevented when the cells were loaded with ruthenium red, a blocker of Ca²⁺-induced Ca²⁺ release (CICR) stores, whereas loading the cells with heparin, a blocker of IP₃-induced Ca²⁺ release stores, had no effect. The effects of ryanodine, another compound that interferes with CICR stores, were also investigated. Intracellular injection of ryanodine prevented the induction of LTD_GABA-A only when the TS was preceded by depolarizing pulses that increase intracellular Ca²⁺ concentration. When applied in the bath, ryanodine prevented the induction of LTD_GABA-A. Altogether, these results suggest that ryanodine acts as a Ca²⁺-dependent blocker of CICR stores and that the induction of LTD_GABA-A required the activation of both presynaptic and postsynaptic CICR stores.

Key words: synaptic plasticity; development; GABA; glutamate; calcium stores; hippocampus

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Copyright © 0000 Society for Neuroscience  0270-6474/00/$05.00/0

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