 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, October 1, 2000, 20(19):7131-7142
Active Hair Bundle Motion Linked to Fast Transducer Adaptation in Auditory Hair Cells
A. J. Ricci1, 2, A. C. Crawford3, and R. Fettiplace1 1 Department of Physiology, University of Wisconsin Medical School, Madison, Wisconsin 53706, 2 Neuroscience Center, Louisiana State University Medical Center, New Orleans, Louisiana 70112, and 3 Department of Physiology, University of Cambridge, Cambridge CB2 3EG, United Kingdom
During transduction in auditory hair cells, hair bundle deflection opens mechanotransducer channels that subsequently reclose or adapt to maintained stimuli, a major component of the adaptation occurring on a submillisecond time scale. Using a photodiode imaging technique, we measured hair bundle motion in voltage-clamped turtle hair cells to search for a mechanical correlate of fast adaptation. Excitatory force steps imposed by a flexible glass fiber attached to the bundle caused an initial movement toward the kinocilium, followed by a fast recoil equivalent to bundle stiffening. The recoil had a time course identical to adaptation of the transducer current, and like adaptation, was most prominent for small stimuli, was slowed by reducing extracellular calcium, and varied with hair cell resonant frequency. In free-standing hair bundles, depolarizations positive to 0 mV evoked an outward current attributable to opening of transducer channels, which was accompanied by a sustained bundle deflection toward the kinocilium. Both processes were sensitive to external calcium concentration and were abolished by blocking the transducer channels with dihydrostreptomycin. The similarity in properties of fast adaptation and the associated bundle motion indicates the operation of a rapid calcium-sensitive force generator linked to the gating of the transducer channels. This force generator may permit stimulus amplification during transduction in auditory hair cells.
Key words: adaptation; hair cell; cochlea; hair bundle motility; mechanoelectrical transduction; photodiode imaging
Copyright © 2000 Society for Neuroscience 0270-6474/00/20197131-12$05.00/0
This article has been cited by other articles:
R. Stepanyan and G. I. Frolenkov
Fast Adaptation and Ca2+ Sensitivity of the Mechanotransducer Require Myosin-XVa in Inner But Not Outer Cochlear Hair Cells
J. Neurosci., April 1, 2009; 29(13): 4023 - 4034.
[Abstract] [Full Text] [PDF]
W. M. Roberts and M. A. Rutherford
Linear and nonlinear processing in hair cells
J. Exp. Biol., June 1, 2008; 211(11): 1775 - 1780.
[Abstract] [Full Text] [PDF]
S. Jia, P. Dallos, and D. Z. Z. He
Mechanoelectric Transduction of Adult Inner Hair Cells
J. Neurosci., January 31, 2007; 27(5): 1006 - 1014.
[Abstract] [Full Text] [PDF]
R. Fettiplace
Active hair bundle movements in auditory hair cells
J. Physiol., October 1, 2006; 576(1): 29 - 36.
[Abstract] [Full Text] [PDF]
H. J. Kennedy, M. G. Evans, A. C. Crawford, and R. Fettiplace
Depolarization of cochlear outer hair cells evokes active hair bundle motion by two mechanisms.
J. Neurosci., March 8, 2006; 26(10): 2757 - 2766.
[Abstract] [Full Text] [PDF]
A. J. Ricci, H. J. Kennedy, A. C. Crawford, and R. Fettiplace
The Transduction Channel Filter in Auditory Hair Cells
J. Neurosci., August 24, 2005; 25(34): 7831 - 7839.
[Abstract] [Full Text] [PDF]
S. M. Highstein, R. D. Rabbitt, G. R. Holstein, and R. D. Boyle
Determinants of Spatial and Temporal Coding by Semicircular Canal Afferents
J Neurophysiol, May 1, 2005; 93(5): 2359 - 2370.
[Abstract] [Full Text] [PDF]
K. Nagata, A. Duggan, G. Kumar, and J. Garcia-Anoveros
Nociceptor and Hair Cell Transducer Properties of TRPA1, a Channel for Pain and Hearing
J. Neurosci., April 20, 2005; 25(16): 4052 - 4061.
[Abstract] [Full Text] [PDF]
R. D. Rabbitt, R. Boyle, G. R. Holstein, and S. M. Highstein
Hair-Cell Versus Afferent Adaptation in the Semicircular Canals
J Neurophysiol, January 1, 2005; 93(1): 424 - 436.
[Abstract] [Full Text] [PDF]
L. Catacuzzeno, B. Fioretti, P. Perin, and F. Franciolini
Spontaneous low-frequency voltage oscillations in frog saccular hair cells
J. Physiol., December 15, 2004; 561(3): 685 - 701.
[Abstract] [Full Text] [PDF]
B. Nadrowski, P. Martin, and F. Julicher
Active hair-bundle motility harnesses noise to operate near an optimum of mechanosensitivity
PNAS, August 17, 2004; 101(33): 12195 - 12200.
[Abstract] [Full Text] [PDF]
H. E. Farris, C. L. LeBlanc, J. Goswami, and A. J. Ricci
Probing the pore of the auditory hair cell mechanotransducer channel in turtle
J. Physiol., August 1, 2004; 558(3): 769 - 792.
[Abstract] [Full Text] [PDF]
M. A. Vollrath and R. A. Eatock
Time Course and Extent of Mechanotransducer Adaptation in Mouse Utricular Hair Cells: Comparison With Frog Saccular Hair Cells
J Neurophysiol, October 1, 2003; 90(4): 2676 - 2689.
[Abstract] [Full Text] [PDF]
P. Martin, D. Bozovic, Y. Choe, and A. J. Hudspeth
Spontaneous Oscillation by Hair Bundles of the Bullfrog's Sacculus
J. Neurosci., June 1, 2003; 23(11): 4533 - 4548.
[Abstract] [Full Text] [PDF]
V. Rybalchenko and J. Santos-Sacchi
Cl- flux through a non-selective, stretch-sensitive conductance influences the outer hair cell motor of the guinea-pig
J. Physiol., March 15, 2003; 547(3): 873 - 891.
[Abstract] [Full Text] [PDF]
D. Bozovic and A. J. Hudspeth
Hair-bundle movements elicited by transepithelial electrical stimulation of hair cells in the sacculus of the bullfrog
PNAS, February 4, 2003; 100(3): 958 - 963.
[Abstract] [Full Text] [PDF]
D. Z Z He, K. W Beisel, L. Chen, D.-L. Ding, S. Jia, B. Fritzsch, and R. Salvi
Chick hair cells do not exhibit voltage-dependent somatic motility
J. Physiol., January 15, 2003; 546(2): 511 - 520.
[Abstract] [Full Text] [PDF]
J. Ashmore
Biophysics of the cochlea - biomechanics and ion channelopathies
Br. Med. Bull., October 1, 2002; 63(1): 59 - 72.
[Abstract] [Full Text] [PDF]
A. Ricci
Differences in Mechano-Transducer Channel Kinetics Underlie Tonotopic Distribution of Fast Adaptation in Auditory Hair Cells
J Neurophysiol, April 1, 2002; 87(4): 1738 - 1748.
[Abstract] [Full Text] [PDF]
A. J. Ricci, A. C. Crawford, and R. Fettiplace
Mechanisms of Active Hair Bundle Motion in Auditory Hair Cells
J. Neurosci., January 1, 2002; 22(1): 44 - 52.
[Abstract] [Full Text] [PDF]
P. Martin, A. J. Hudspeth, and F. Julicher
Comparison of a hair bundle's spontaneous oscillations with its response to mechanical stimulation reveals the underlying active process
PNAS, November 20, 2001; (2001) 251530598.
[Abstract] [Full Text] [PDF]
G. A. Manley
Evidence for an Active Process and a Cochlear Amplifier in Nonmammals
J Neurophysiol, August 1, 2001; 86(2): 541 - 549.
[Abstract] [Full Text] [PDF]
R. A. Dumont, U. Lins, A. G. Filoteo, J. T. Penniston, B. Kachar, and P. G. Gillespie
Plasma Membrane Ca2+-ATPase Isoform 2a Is the PMCA of Hair Bundles
J. Neurosci., July 15, 2001; 21(14): 5066 - 5078.
[Abstract] [Full Text] [PDF]
G. A. Manley, D. L. Kirk, C. Köppl, and G. K. Yates
In vivo evidence for a cochlear amplifier in the hair-cell bundle of lizards
PNAS, February 8, 2001; (2001) 41604998.
[Abstract] [Full Text]
G. A. Manley, D. L. Kirk, C. Koppl, and G. K. Yates
In vivo evidence for a cochlear amplifier in the hair-cell bundle of lizards
PNAS, February 27, 2001; 98(5): 2826 - 2831.
[Abstract] [Full Text] [PDF]
P. Martin, A. J. Hudspeth, and F. Julicher
Comparison of a hair bundle's spontaneous oscillations with its response to mechanical stimulation reveals the underlying active process
PNAS, December 4, 2001; 98(25): 14380 - 14385.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|