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The Journal of Neuroscience, October 1, 2000, 20(19):7193-7198

Coordinated Gating of TRP-Dependent Channels in Rhabdomeral Membranes from Drosophila Retinas

Joan E. Haab¹, Cecilia Vergara², Juan Bacigalupo², and Peter M. O'Day¹

¹ Institute of Neuroscience and Department of Biology, University of Oregon, Eugene, Oregon 97403, and ² Department of Biology, Facultad de Ciencias, University of Chile, Santiago, Chile

Using a newly developed dissociation procedure, we isolated the specialized rhabdomeral membranes from Drosophila retinal photoreceptors. From these membranes, we have recorded spontaneous active currents in excised patch, voltage-clamp recordings. We observed rapid opening events that closely resembled those ascribed to one class of light-activated channels, TRP. All activity exhibited Ba²⁺ permeability, little voltage dependence, and sensitivity to La³⁺ block. Mutational analysis indicated that the spontaneous activity present in these membranes was TRP-dependent. Excised patches from wild-type rhabdomeral membranes exhibited a wide range of conductance amplitudes. In addition, large conductance events exhibited many conductance levels in the open state. Block of activity by La³⁺ both developed and recovered in a stepwise manner. Our results indicate that TRP-dependent channels have a small unitary conductance and that many channels can be gated coordinately.

Key words: Drosophila; TRP; phototransduction; channel; barium; rhabdomere

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Copyright © 2000 Society for Neuroscience  0270-6474/00/20197193-06$05.00/0

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