 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
The Journal of Neuroscience, January 15, 2000, 20(2):649-659
Novel Mechanism of Massive Photoreceptor Degeneration Caused by Mutations in the trp Gene of Drosophila
Jaeseung Yoon2, Hagit Cohen Ben-Ami3, Young Seok Hong1, Soyeon Park2, Lydia L. R. Strong1, John Bowman1, Chaoxian Geng1, Kwanghee Baek2, Baruch Minke3, and William L. Pak1 1 Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, 2 Institute of Genetic Engineering and Natural Sciences, Department of Genetic Engineering, KyungHee University, Yongin City, Kyungki-Do, 449-701 Korea, and 3 Department of Physiology and the Kuhne Minerva Center for Studies of Visual Transduction, The Hebrew University, Hadassah Medical School, Jerusalem, Israel
The Drosophila trp gene encodes a light-activated Ca2+ channel subunit, which is a prototypical member of a novel class of channel proteins. Previously identified trp mutants are all recessive, loss-of-function mutants characterized by a transient receptor potential and the total or near-total loss of functional TRP protein. Although retinal degeneration does occur in these mutants, it is relatively mild and slow in onset. We report herein a new mutant, TrpP365, that does not display the transient receptor potential phenotype and is characterized by a substantial level of the TRP protein and rapid, semi-dominant degeneration of photoreceptors. We show that, in spite of its unusual phenotypes, TrpP365 is a trp allele because a TrpP365 transgene induces the mutant phenotype in a wild-type background, and a wild-type trp transgene in a TrpP365 background suppresses the mutant phenotype. Moreover, amino acid alterations that could cause the TrpP365 phenotype are found in the transmembrane segment region of the mutant channel protein. Whole-cell recordings clarified the mechanism underlying the retinal degeneration by showing that the TRP channels of TrpP365 are constitutively active. Although several genes, when mutated, have been shown to cause retinal degeneration in Drosophila, the underlying mechanism has not been identified for any of them. The present studies provide evidence for a specific mechanism for massive degeneration of photoreceptors in Drosophila. Insofar as some human homologs of TRP are highly expressed in the brain, a similar mechanism could be a major contributor to degenerative disorders of the brain.
Key words: TRP Ca2+ channel; photoreceptor degeneration; novel mechanism of neuronal cell death; semi-dominant trp mutant; constitutive channel activity; Drosophila
Copyright © 2000 Society for Neuroscience 0270-6474/00/202649-11$05.00/0
This article has been cited by other articles:
R. M. Sappington and D. J. Calkins
Contribution of TRPV1 to Microglia-Derived IL-6 and NF{kappa}B Translocation with Elevated Hydrostatic Pressure
Invest. Ophthalmol. Vis. Sci., July 1, 2008; 49(7): 3004 - 3017.
[Abstract] [Full Text] [PDF]
Z. Su, X. Zhou, W. J. Haynes, S. H. Loukin, A. Anishkin, Y. Saimi, and C. Kung
Yeast gain-of-function mutations reveal structure function relationships conserved among different subfamilies of transient receptor potential channels
PNAS, December 4, 2007; 104(49): 19607 - 19612.
[Abstract] [Full Text] [PDF]
D. Kalra, R. Elsaesser, Y. Gu, and K. Venkatachalam
Transducin in Rod Photoreceptors: Translocated When Not Terminated
J. Neurosci., June 13, 2007; 27(24): 6349 - 6351.
[Full Text] [PDF]
A. M. Jose, I. A. Bany, D. L. Chase, and M. R. Koelle
A Specific Subset of Transient Receptor Potential Vanilloid-Type Channel Subunits in Caenorhabditis elegans Endocrine Cells Function as Mixed Heteromers to Promote Neurotransmitter Release
Genetics, January 1, 2007; 175(1): 93 - 105.
[Abstract] [Full Text] [PDF]
M. Richard, R. Roepman, W. M. Aartsen, A. G.S.H. van Rossum, A. I. den Hollander, E. Knust, J. Wijnholds, and F. P.M. Cremers
Towards understanding CRUMBS function in retinal dystrophies
Hum. Mol. Genet., October 15, 2006; 15(suppl_2): R235 - R243.
[Abstract] [Full Text] [PDF]
M. A. Cronin, M.-H. Lieu, and S. Tsunoda
Two stages of light-dependent TRPL-channel translocation in Drosophila photoreceptors
J. Cell Sci., July 15, 2006; 119(14): 2935 - 2944.
[Abstract] [Full Text] [PDF]
N. E. Meyer, T. Joel-Almagor, S. Frechter, B. Minke, and A. Huber
Subcellular translocation of the eGFP-tagged TRPL channel in Drosophila photoreceptors requires activation of the phototransduction cascade
J. Cell Sci., June 15, 2006; 119(12): 2592 - 2603.
[Abstract] [Full Text] [PDF]
T. Wang, Y. Jiao, and C. Montell
Dissecting independent channel and scaffolding roles of the Drosophila transient receptor potential channel
J. Cell Biol., November 21, 2005; 171(4): 685 - 694.
[Abstract] [Full Text] [PDF]
A. M. Celotto and M. J. Palladino
Drosophila: A "Model" Model System To Study Neurodegeneration
Mol. Interv., October 1, 2005; 5(5): 292 - 303.
[Abstract] [Full Text] [PDF]
C. Montell
TRP channels in Drosophila photoreceptor cells
J. Physiol., August 15, 2005; 567(1): 45 - 51.
[Abstract] [Full Text] [PDF]
M. M. Aarts and M. Tymianski
TRPM7 and Ischemic CNS Injury
Neuroscientist, April 1, 2005; 11(2): 116 - 123.
[Abstract] [PDF]
P. Georgiev, I. Garcia-Murillas, D. Ulahannan, R. C. Hardie, and P. Raghu
Functional INAD complexes are required to mediate degeneration in photoreceptors of the Drosophila rdgA mutant
J. Cell Sci., April 1, 2005; 118(7): 1373 - 1384.
[Abstract] [Full Text] [PDF]
C. Montell
The TRP Superfamily of Cation Channels
Sci. Signal., February 22, 2005; 2005(272): re3 - re3.
[Abstract] [Full Text] [PDF]
L. Zhang and G. J. Barritt
Evidence that TRPM8 Is an Androgen-Dependent Ca2+ Channel Required for the Survival of Prostate Cancer Cells
Cancer Res., November 15, 2004; 64(22): 8365 - 8373.
[Abstract] [Full Text] [PDF]
R. Iakhine, I. Chorna-Ornan, T. Zars, N. Elia, Y. Cheng, Z. Selinger, B. Minke, and D. R. Hyde
Novel Dominant Rhodopsin Mutation Triggers Two Mechanisms of Retinal Degeneration and Photoreceptor Desensitization
J. Neurosci., March 10, 2004; 24(10): 2516 - 2526.
[Abstract] [Full Text] [PDF]
Y. S. Hong, S. Park, C. Geng, K. Baek, J. D. Bowman, J. Yoon, and W. L. Pak
Single Amino Acid Change in the Fifth Transmembrane Segment of the TRP Ca2+ Channel Causes Massive Degeneration of Photoreceptors
J. Biol. Chem., September 6, 2002; 277(37): 33884 - 33889.
[Abstract] [Full Text] [PDF]
B. Minke and B. Cook
TRP Channel Proteins and Signal Transduction
Physiol Rev, April 1, 2002; 82(2): 429 - 472.
[Abstract] [Full Text] [PDF]
P. Lane, G. Hao, and S. S. Gross
S-Nitrosylation Is Emerging as a Specific and Fundamental Posttranslational Protein Modification: Head-to-Head Comparison with O-Phosphorylation
Sci. Signal., June 12, 2001; 2001(86): re1 - re1.
[Abstract] [Full Text] [PDF]
K. Agam, M. von Campenhausen, S. Levy, H. C. Ben-Ami, B. Cook, K. Kirschfeld, and B. Minke
Metabolic Stress Reversibly Activates the Drosophila Light-Sensitive Channels TRP and TRPL In Vivo
J. Neurosci., August 1, 2000; 20(15): 5748 - 5755.
[Abstract] [Full Text] [PDF]
S.-E. Jordt, M. Tominaga, and D. Julius
Acid potentiation of the capsaicin receptor determined by a key extracellular site
PNAS, June 14, 2000; (2000) 100129497.
[Abstract] [Full Text]
S.-E. Jordt, M. Tominaga, and D. Julius
Acid potentiation of the capsaicin receptor determined by a key extracellular site
PNAS, July 5, 2000; 97(14): 8134 - 8139.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|