WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience Join the Society for Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Waters, J.
Right arrow Articles by Smith, S. J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Waters, J.
Right arrow Articles by Smith, S. J

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 2000, 20(21):7863-7870

Phorbol Esters Potentiate Evoked and Spontaneous Release by Different Presynaptic Mechanisms

Jack Waters and Stephen J Smith

Department of Molecular and Cellular Physiology, Beckman Center, Stanford Medical School, Stanford, California 94305

Phorbol esters enhance release from a variety of cell types. The mechanism by which phorbol esters potentiate presynaptic release from central neurons is unclear, although effects of phorbol esters both on the readily releasable pool of vesicles and on presynaptic calcium channels have been shown. Using confocal microscopy and the fluorescent styryl dye FM 1-43, we have examined the effects of phorbol-12,13-dibutyrate (PDBu) on presynaptic vesicle turnover at individually identified synapses in dissociated cultures obtained from neonatal rat hippocampus. Using different dye staining and destaining protocols we were able to resolve two effects of PDBu. Potentiation of evoked release by PDBu was insensitive to calcium channel antagonists, suggesting that this effect results from an increased number of vesicles in the readily releasable pool. Since we observed no effect of PDBu on the size of the total recycling vesicle pool, we conclude that phorbol esters alter the equilibrium between reserve and readily releasable pools. An additional effect of PDBu on spontaneous release was observed. This effect was antagonized by nifedipine but not omega -conotoxin GVIA or omega -agatoxin IVA. We conclude that PDBu influences spontaneous and evoked release by two different mechanisms: through L-type calcium channels and through an increase in the proportion of recycling vesicles in the readily releasable pool. In addition to further clarifying the mechanism of action of phorbol esters, these results suggest that phorbol esters may be a useful tool with which to probe the function of the readily releasable pool of presynaptic vesicles at CNS synapses.

Key words: hippocampus; neuron; L-type calcium channel; readily releasable pool; vesicle; FM 1-43; protein kinase; release probability

Copyright © 2000 Society for Neuroscience  0270-6474/00/20217863-08$05.00/0


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
X. Lou, N. Korogod, N. Brose, and R. Schneggenburger
Phorbol Esters Modulate Spontaneous and Ca2+-Evoked Transmitter Release via Acting on Both Munc13 and Protein Kinase C
J. Neurosci., August 13, 2008; 28(33): 8257 - 8267.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
I. Gekel and E. Neher
Application of an Epac Activator Enhances Neurotransmitter Release at Excitatory Central Synapses
J. Neurosci., August 6, 2008; 28(32): 7991 - 8002.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. Kron, M. Morschel, J. Reuter, W. Zhang, and M. Dutschmann
Developmental changes in brain-derived neurotrophic factor-mediated modulations of synaptic activities in the pontine Kolliker Fuse nucleus of the rat
J. Physiol., August 15, 2007; 583(1): 315 - 327.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
G. Houeland, A. Nakhost, W. S. Sossin, and V. F. Castellucci
PKC Modulation of Transmitter Release by SNAP-25 at Sensory-to-Motor Synapses in Aplysia
J Neurophysiol, January 1, 2007; 97(1): 134 - 143.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. D. Tompkins and R. L. Parsons
Exocytotic release of ATP and activation of P2X receptors in dissociated guinea pig stellate neurons
Am J Physiol Cell Physiol, November 1, 2006; 291(5): C1062 - C1071.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
W. J. Tyler, X.-l. Zhang, K. Hartman, J. Winterer, W. Muller, P. K. Stanton, and L. Pozzo-Miller
BDNF increases release probability and the size of a rapidly recycling vesicle pool within rat hippocampal excitatory synapses
J. Physiol., August 1, 2006; 574(3): 787 - 803.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H.-F. Guo and Y. Zhong
Requirement of Akt to Mediate Long-Term Synaptic Depression in Drosophila
J. Neurosci., April 12, 2006; 26(15): 4004 - 4014.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
N. K. Charlie, M. A. Schade, A. M. Thomure, and K. G. Miller
Presynaptic UNC-31 (CAPS) Is Required to Activate the G{alpha}s Pathway of the Caenorhabditis elegans Synaptic Signaling Network
Genetics, February 1, 2006; 172(2): 943 - 961.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
T. Virmani, M. Ertunc, Y. Sara, M. Mozhayeva, and E. T. Kavalali
Phorbol Esters Target the Activity-Dependent Recycling Pool and Spare Spontaneous Vesicle Recycling
J. Neurosci., November 23, 2005; 25(47): 10922 - 10929.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G.-r. Zhang, X. Wang, L. Kong, X.-g. Lu, B. Lee, M. Liu, M. Sun, C. Franklin, R. G. Cook, and A. I. Geller
Genetic Enhancement of Visual Learning by Activation of Protein Kinase C Pathways in Small Groups of Rat Cortical Neurons
J. Neurosci., September 14, 2005; 25(37): 8468 - 8481.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. A. Schade, N. K. Reynolds, C. M. Dollins, and K. G. Miller
Mutations That Rescue the Paralysis of Caenorhabditis elegans ric-8 (Synembryn) Mutants Activate the G{alpha}s Pathway and Define a Third Major Branch of the Synaptic Signaling Network
Genetics, February 1, 2005; 169(2): 631 - 649.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
N. K. Reynolds, M. A. Schade, and K. G. Miller
Convergent, RIC-8-Dependent G{alpha} Signaling Pathways in the Caenorhabditis elegans Synaptic Signaling Network
Genetics, February 1, 2005; 169(2): 651 - 670.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Y. Zhao and M. Klein
Changes in the Readily Releasable Pool of Transmitter and in Efficacy of Release Induced by High-Frequency Firing at Aplysia Sensorimotor Synapses in Culture
J Neurophysiol, April 1, 2004; 91(4): 1500 - 1509.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Coco, F. Calegari, E. Pravettoni, D. Pozzi, E. Taverna, P. Rosa, M. Matteoli, and C. Verderio
Storage and Release of ATP from Astrocytes in Culture
J. Biol. Chem., January 3, 2003; 278(2): 1354 - 1362.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Brose and E. Neher
Specificity emerges in the dissection of diacylglycerol- and protein kinase C-mediated signalling pathways
PNAS, December 24, 2002; 99(26): 16522 - 16523.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Yang, S. Udayasankar, J. Dunning, P. Chen, and K. D. Gillis
A highly Ca2+-sensitive pool of vesicles is regulated by protein kinase C in adrenal chromaffin cells
PNAS, December 24, 2002; 99(26): 17060 - 17065.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Zhao and M. Klein
Modulation of the Readily Releasable Pool of Transmitter and of Excitation-Secretion Coupling by Activity and by Serotonin at Aplysia Sensorimotor Synapses in Culture
J. Neurosci., December 15, 2002; 22(24): 10671 - 10679.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Brose and C. Rosenmund
Move over protein kinase C, you've got company: alternative cellular effectors of diacylglycerol and phorbol esters
J. Cell Sci., January 12, 2002; 115(23): 4399 - 4411.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
W. J. Tyler and L. D. Pozzo-Miller
BDNF Enhances Quantal Neurotransmitter Release and Increases the Number of Docked Vesicles at the Active Zones of Hippocampal Excitatory Synapses
J. Neurosci., June 15, 2001; 21(12): 4249 - 4258.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-