QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (28) Citing Articles via Google Scholar Google Scholar Articles by Cragg, S. J. Articles by Greenfield, S. A. Search for Related Content PubMed PubMed Citation Articles by Cragg, S. J. Articles by Greenfield, S. A. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL DOPAMINE MAGNESIUM COMPOUNDS MAGNESIUM, ELEMENTAL

 Previous Article  |  Next Article 

The Journal of Neuroscience, November 1, 2000, 20(21):8209-8217

Dopamine Release and Uptake Dynamics within Nonhuman Primate Striatum In Vitro

Stephanie J. Cragg, Christopher J. Hille, and Susan A. Greenfield

University Department of Pharmacology, Oxford, OX1 3QT, United Kingdom

The putamen of the human striatum is a heterogeneous nucleus that contains the primary site of loss of dopamine (DA) in Parkinson's disease (PD). Furthermore, different functional domains of the putamen are heterogeneously susceptible to DA loss, and yet the dynamic regulation of extracellular DA concentration ([DA]_o) and comparison between domains has not been explored in the primate brain. In these studies, DA was measured in real time using fast-scan cyclic voltammetry at a carbon-fiber microelectrode in vitro in striatal sections from the common marmoset (Callithrix jacchus). [DA]_o released by a single stimulus pulse varied threefold along a ventromedial-dorsolateral axis. DA uptake was via the DA transporter (GBR12909 sensitive, desipramine insensitive). On the basis of data modeling with simulations of Michaelis-Menten kinetics, rate maximum, V_max, varied with region: both [DA]_o and V_max were greatest in regions most vulnerable in PD. These differences were reflected in part by regional variation in DA content. [DA]_o, V_max, and regional variation were two- to threefold greater than in rodent caudatoputamen.

In addition, steady-state [DA]_o at physiological firing rates in primate striatum was controlled by depolarization frequency, uptake, and presynaptic autoreceptors. Furthermore, regulation of [DA]_o by these mechanisms differed significantly between limbic- and motor-associated domains.

These data indicate interspecies heterogeneity in striatal DA dynamics that must be considered when extrapolating behavioral and drug responses from rodent to the primate brain. Moreover, the heterogeneity demonstrated within the primate putamen in the availability and dynamic regulation of DA may be central to understanding DA function in health, cocaine abuse, and disease.

Key words: Parkinson's disease; basal ganglia; DA transporter; DA uptake; autoreceptor; cocaine

---

Copyright © 2000 Society for Neuroscience  0270-6474/00/20218209-09$05.00/0

This article has been cited by other articles:

				S. N. Blythe, J. F. Atherton, and M. D. Bevan Synaptic Activation of Dendritic AMPA and NMDA Receptors Generates Transient High-Frequency Firing in Substantia Nigra Dopamine Neurons In Vitro J Neurophysiol, April 1, 2007; 97(4): 2837 - 2850. [Abstract] [Full Text] [PDF]

				S. E. McCallum, N. Parameswaran, T. Bordia, J. M. McIntosh, S. R. Grady, and M. Quik Decrease in {alpha}3*/{alpha}6* Nicotinic Receptors but Not Nicotine-Evoked Dopamine Release in Monkey Brain after Nigrostriatal Damage Mol. Pharmacol., September 1, 2005; 68(3): 737 - 746. [Abstract] [Full Text] [PDF]

				M. A. Nader and P. W. Czoty PET Imaging of Dopamine D2 Receptors in Monkey Models of Cocaine Abuse: Genetic Predisposition Versus Environmental Modulation Am J Psychiatry, August 1, 2005; 162(8): 1473 - 1482. [Abstract] [Full Text] [PDF]

				S. D. Gale and D. J. Perkel Properties of Dopamine Release and Uptake in the Songbird Basal Ganglia J Neurophysiol, April 1, 2005; 93(4): 1871 - 1879. [Abstract] [Full Text] [PDF]

				L. J. Porrino, D. Lyons, H. R. Smith, J. B. Daunais, and M. A. Nader Cocaine Self-Administration Produces a Progressive Involvement of Limbic, Association, and Sensorimotor Striatal Domains J. Neurosci., April 7, 2004; 24(14): 3554 - 3562. [Abstract] [Full Text] [PDF]

				J. Hahn, T. E. Tse, and E. S. Levitan Long-Term K+ Channel-Mediated Dampening of Dopamine Neuron Excitability by the Antipsychotic Drug Haloperidol J. Neurosci., November 26, 2003; 23(34): 10859 - 10866. [Abstract] [Full Text] [PDF]

				S. J. Cragg Variable Dopamine Release Probability and Short-Term Plasticity between Functional Domains of the Primate Striatum J. Neurosci., May 15, 2003; 23(10): 4378 - 4385. [Abstract] [Full Text] [PDF]

				S. J. Cragg, C. J. Hille, and S. A. Greenfield Functional Domains in Dorsal Striatum of the Nonhuman Primate Are Defined by the Dynamic Behavior of Dopamine J. Neurosci., July 1, 2002; 22(13): 5705 - 5712. [Abstract] [Full Text] [PDF]

				Q. Wu, M. E. A. Reith, M. J. Kuhar, F. I. Carroll, and P. A. Garris Preferential Increases in Nucleus Accumbens Dopamine after Systemic Cocaine Administration Are Caused by Unique Characteristics of Dopamine Neurotransmission J. Neurosci., August 15, 2001; 21(16): 6338 - 6347. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help