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The Journal of Neuroscience, November 15, 2000, 20(22):8269-8278
Identification of the Kainate Receptor Subunits Underlying
Modulation of Excitatory Synaptic Transmission in the CA3 Region of the
Hippocampus
Anis
Contractor1,
Geoffrey T.
Swanson1,
Andreas
Sailer1,
Stephen
O'Gorman2, and
Stephen F.
Heinemann1
1 Molecular Neurobiology Laboratory and
2 Gene Expression Laboratory, The Salk Institute for
Biological Studies, La Jolla, California 92037
To understand the physiological role of kainate receptors and their
participation in seizure induction in animal models of epilepsy, it
will be necessary to develop a comprehensive description of their
action in the CA3 region of the hippocampus. Activation of presynaptic
kainate receptors depresses excitatory synaptic transmission at mossy
fiber and associational-commissural inputs to CA3 pyramidal neurons
(Vignes et al., 1998; Bortolotto et al., 1999; Kamiya and Ozawa, 2000).
In this study, we use gene-targeted mice lacking glutamate receptor 5 (GluR5) or GluR6 kainate receptor subunits to identify the
receptor subunits that comprise the kainate receptors responsible for
presynaptic modulation of CA3 transmission. We found that bath
application of kainate (3 µM) profoundly reduced EPSCs at mossy fiber and collateral synapses in neurons from
wild-type and GluR5 / mice but
had no effect on EPSCs in neurons from
GluR6/ mice. These results
therefore contrast with previous studies that supported a role for
GluR5-containing receptors at mossy fiber and associational-commissural
synapses (Vignes et al., 1998; Bortolotto et al., 1999). Surprisingly,
at perforant path synapses kainate receptor activation enhanced
transmission; this potentiation was abolished in both GluR5 and GluR6
knock-out mice. Kainate receptors thus play multiple and complex roles
to modulate excitatory synaptic transmission in the CA3 region of the hippocampus.
Key words:
presynaptic kainate receptors; CA3 pyramidal neurons; kainate receptor knock-out mice; hippocampus; mossy fiber; excitatory
synaptic transmission
Copyright © 2000 Society for Neuroscience 0270-6474/00/20228269-10$05.00/0
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